Physical degradation of proteins in well-defined fluid flows studied within a four-roll apparatus

Biotechnology and Bioengineering

Volumn 108, Issue 12, 2011, Pages 2914-2922

  Simon, S ^a   Krause, H J ^b   Weber, C ^b   Peukert, W ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b ABBOTT GMBH AND CO KG   (Germany)

Author keywords

Denaturation; Elongation; Monitoring of aggregation; Protein adsorption; Protein aggregation; Shear

Indexed keywords

ABSORBANCES; AGGREGATE FORMATION; ALCOHOL DEHYDROGENASE; BIOPHARMACEUTICALS; BOVINE SERUM ALBUMINS; DOWNSTREAM-PROCESSING; ELONGATION RATES; EXPERIMENTAL SETUP; FLOW CONDITION; FLOW CONFIGURATIONS; FLUID STRESS; FORMULATION DEVELOPMENT; HIGH REPRODUCIBILITY; HYDRODYNAMIC STRESS; IN-SITU; LOW STRESS; MODEL PROTEINS; PARTICLE IMAGE VELOCIMETRIES; PROTEIN ADSORPTION; PROTEIN AGGREGATES; PROTEIN AGGREGATION; SCREENING EXPERIMENTS; SHEAR; TURBIDITY MEASUREMENTS;

ADSORPTION; BIODEGRADATION; BODY FLUIDS; COMPUTATIONAL FLUID DYNAMICS; DEGRADATION; ENZYMES; EXPERIMENTS; FLUIDS; PROTEINS; SHEAR FLOW; SHEAR STRAIN; STRAIN RATE; STRESS CONCENTRATION; TURBIDITY; VELOCITY MEASUREMENT;

FLOW OF FLUIDS;

ALCOHOL DEHYDROGENASE; BOVINE SERUM ALBUMIN; LYSOZYME;

ABSORPTION; ADSORPTION; APPARATUS; ARTICLE; BIOTECHNOLOGY; COMPUTATIONAL FLUID DYNAMICS; FLUID FLOW; PARTICLE IMAGE VELOCIMETRY; PARTICLE SIZE; PROTEIN AGGREGATION; PROTEIN DEGRADATION; REPRODUCIBILITY; SHEAR STRESS; TURBIDITY;

BIOTECHNOLOGY; HYDRODYNAMICS; PROTEINS; PROTEOLYSIS; SPECTROPHOTOMETRY;

BOVINAE;

EID: 80054036751     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23257     Document Type: Article

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