Establishment of CRISPR/Cas9 in Alternaria alternata

Fungal Genetics and Biology

Volumn 101, Issue , 2017, Pages 55-60

  Wenderoth, Maximilian ^a   Pinecker, Christoph ^a   Voß, Benjamin ^a   Fischer, Reinhard ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Alternaria; CRISPR; Knock out; Uracil

Indexed keywords

GREEN FLUORESCENT PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR STUA; UNCLASSIFIED DRUG; ALTERNARIOL; FUNGAL PROTEIN; LACTONE; MELANIN; OROTIDINE 5' PHOSPHATE DECARBOXYLASE;

ALTERNARIA ALTERNATA; ARTICLE; ASPERGILLUS FUMIGATUS; ASPERGILLUS NIDULANS; BRM2 GENE; CONTROLLED STUDY; CRISPR CAS SYSTEM; FUNGAL GENE; FUNGAL STRAIN; GENE DELETION; GENE INACTIVATION; GENE MUTATION; INOCULATION; MELANOGENESIS; NEUROSPORA CRASSA; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PKSA GENE; PROTOPLAST; PYR 4 GENE; PYRG GENE; ALTERNARIA; ANTAGONISTS AND INHIBITORS; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; FUNGUS SPORE; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLIC ENGINEERING; METABOLISM; MUTATION; SECONDARY METABOLISM;

ALTERNARIA; CRISPR-CAS SYSTEMS; FUNGAL PROTEINS; LACTONES; MELANINS; METABOLIC ENGINEERING; MUTATION; NEUROSPORA CRASSA; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; SECONDARY METABOLISM; SPORES, FUNGAL;

EID: 85015342138     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2017.03.001     Document Type: Article

References (34)

1. Bräse, S., et al. Chemistry and biology of mycotoxins and related fungal metabolites. Chem. Rev. 109 (2009), 3903–3990.
2. Chalfie, M., et al. Green fluorescent protein as a marker for gene expression. Science 263 (1994), 802–805.
3. Cho, S.W., et al. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat. Biotechnol. 31 (2013), 230–232.
4. Cho, Y., How the necrotrophic fungus Alternaria brassicicola kills plant cells remains an enigma. Eukaryot. Cell 14 (2015), 335–344.
5. Dang, H.X., et al. The Alternaria genomes database: a comprehensive resource for a fungal genus comprised of saprophytes, plant pathogens, and allergenic species. BMC Genomics, 16, 2015, 239.
6. Estiarte, N., et al. LaeA and VeA are involved in growth morphology, asexual development, and mycotoxin production in Alternaria alternata. Int. J. Food Microbiol. 238 (2016), 153–164.
7. 2 transcription factor CmrA controls melanization and spore development. Microbiology 160 (2014), 1845–1854.
8. Fleck, S.C., et al. DNA damage and repair kinetics of the Alternaria mycotoxins alternariol, altertoxin II and stemphyltoxin III in cultured cells. Mutat. Res., Genet. Toxicol. Environ. Mutagen. 798–799 (2016), 27–34.
9. Fuller, K.K., et al. Development of the CRISPR/Cas9 system for targeted gene disruption in Aspergillus fumigatus. Eukaryot. Cell 14 (2015), 1073–1080.
10. Jakociunas, T., et al. CRISPR/Cas9 advances engineering of microbial cell factories. Metab. Eng. 34 (2016), 44–59.
11. Jinek, M., et al. RNA-programmed genome editing in human cells. Elife, 2, 2013, e00471.
12. Kabak, B., et al. Strategies to prevent mycotoxin contamination of food and animal feed: a review. Crit. Rev. Food Sci. Nutr. 46 (2006), 593–619.
13. Kimura, N., Tsuge, T., Gene cluster involved in melanin biosynthesis of the filamentous fungus Alternaria alternata. J. Bacteriol. 175 (1993), 4427–4435.
14. Kitamoto, N., Matsui, J., Kawai, Y., Kato, A., Yoshino, S., Ohmiya, K., Tsukagoshi, N., Utilization of the TEF1-alpha gene (TEF1) promoter for expression of polygalacturonase genes, pgaA and pgaB, in Aspergillus oryzae. Appl. Microbiol. Biotechnol. 50 (1998), 85–92.
15. Krappmann, S., CRISPR-Cas9, the new kid on the block of fungal molecular biology. Med. Mycol. 55 (2017), 16–23.
16. Kuivanen, J., et al. Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9. Microb. Cell Fact., 15, 2016, 210.
17. Lee, H.B., et al. Alternaria in food: ecophysiology, mycotoxin production and toxicology. Mycobiology 43 (2015), 93–106.
18. Liu, Q., et al. Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering. Biotechnol. Biofuels, 10, 2017, 1.
19. Logrieco, A., et al. Alternaria toxins and plant diseases: an overview of origin, occurrence and risks. World Mycotox. J. 2 (2009), 129–140.
20. Misteli, T., Spector, D.L., Applications of the green fluorescent protein in cell biology and biotechnology. Nat. Biotechnol. 15 (1997), 961–964.
21. Moretti, A., et al. Mycotoxins: an underhand food problem. Methods Mol. Biol. 1542 (2017), 3–12.
22. Nielsen, M.L., et al. Genes linked to production of secondary metabolites in Talaromyces atroroseus revealed using CRISPR-Cas9. PLoS ONE, 12, 2017, e0169712.
23. Nodvig, C.S., et al. A CRISPR-Cas9 system for genetic engineering of filamentous fungi. PLoS ONE, 10, 2015, e0133085.
24. Pruß, S., et al. Role of the Alternaria alternata blue-light receptor WC-1 (LreA) in spore formation and secondary metabolism. Appl. Environ. Microbiol. 80 (2014), 2582–2591.
25. Rodriguez-Carrasco, Y., et al. Development and validation of a LC-ESI-MS/MS method for the determination of Alternaria toxins alternariol, alternariol methyl-ether and tentoxin in tomato and Tamato-based products. Toxins (Basel), 8, 2016, E328.
26. Sambrook, J., Russel, D.W., Molecular Cloning: A Laboratory Manual. 1999, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
27. Schuster, M., et al. Genome editing in Ustilago maydis using the CRISPR-Cas system. Fungal Genet. Biol. 89 (2016), 3–9.
28. Shiotani, H., Tsuge, T., Efficient gene targeting in the filamentous fungus Alternaria alternata. Mol. Gen. Genet. 248 (1995), 142–150.
29. Suelmann, R., et al. Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans. Mol. Microbiol. 25 (1997), 757–769.
30. Tsuge, T., et al. Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev. 37 (2013), 44–66.
31. Vejdovszky, K., et al. In vitro combinatory effects of the Alternaria mycotoxins alternariol and altertoxin II and potentially involved miRNAs. Toxicol. Lett. 267 (2016), 45–52.
32. Weber, J., et al. Functional reconstitution of a fungal natural product gene cluster by advanced genome editing. ACS Synth Biol. 6 (2017), 62–68.
33. Yamagishi, D., et al. G protein signaling mediates developmental processes and pathogenesis of Alternaria alternata. Mol. Plant Microbe Interact. 19 (2006), 1280–1288.
34. Zhang, C., et al. Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus. Fungal Genet. Biol. 86 (2016), 47–57.