Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

PLoS Pathogens

Volumn 13, Issue 2, 2017, Pages

  Burkard, Christine ^a   Lillico, Simon G ^a   Reid, Elizabeth ^b   Jackson, Ben ^b   Mileham, Alan J ^c   Ait Ali, Tahar ^a   Whitelaw, C Bruce A ^a   Archibald, Alan L ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

^c GENUS PLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD14 ANTIGEN; CD16 ANTIGEN; CD163 ANTIGEN; CELL SURFACE MARKER; GENOMIC DNA; GUIDE RNA; HEME OXYGENASE 1; SCAVENGER RECEPTOR; CELL SURFACE RECEPTOR; DIFFERENTIATION ANTIGEN; LEUKOCYTE ANTIGEN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLOOD CELL COUNT; CD163 GENE; CELL DIFFERENTIATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE MICROSCOPY; GENE DELETION; GENE EDITING; GENE EXPRESSION; GENOTYPE; IMMUNOFLUORESCENCE TEST; LUNG ALVEOLUS MACROPHAGE; MACROPHAGE; NONHUMAN; PHENOTYPE; PIG; POLYMERASE CHAIN REACTION; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; VIRUS INFECTIVITY; VIRUS REPLICATION; VIRUS RESISTANCE; VIRUS TRANSCRIPTION; WESTERN BLOTTING; ANIMAL; DEFICIENCY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; GENOME; IMMUNOLOGY; METABOLISM; PROCEDURES; VIROLOGY;

ANIMALS; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BLOTTING, WESTERN; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EDITING; GENOME; GENOTYPE; MACROPHAGES; MICROSCOPY, CONFOCAL; POLYMERASE CHAIN REACTION; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; RECEPTORS, CELL SURFACE; SWINE;

EID: 85014070672     PISSN: 15537366     EISSN: 15537374     Source Type: Journal    
DOI: 10.1371/journal.ppat.1006206     Document Type: Article

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