SeptiFast versus blood culture in clinical routine – A report on 3 years experience

Wiener Klinische Wochenschrift

Volumn 129, Issue 11-12, 2017, Pages 427-434

  Korber, Florian ^a,b   Zeller, Iris ^a   Grünstäudl, Michaela ^a   Willinger, Birgit ^a   Apfalter, Petra ^a,c   Hirschl, Alexander M ^a   Makristathis, Athanasios ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b Praxis Dr med Norbert Ha further   (Germany)

^c ELISABETHINEN HOSPITAL   (Austria)

Author keywords

Blood culture; Diagnostics; Routine use; Sepsis; SeptiFast

Indexed keywords

ANTIBIOTIC AGENT;

ACINETOBACTER BAUMANNII; ARTICLE; ASPERGILLUS FUMIGATUS; BACTEROIDES; BLOOD CULTURE; CANDIDA; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; COMAMONAS TESTOSTERONI; CRITICALLY ILL PATIENT; DIAGNOSTIC TEST ACCURACY STUDY; EMERGENCY CARE; ENDOCARDITIS; ENTEROBACTER; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FEVER; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LISTERIA MONOCYTOGENES; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; OBSERVATIONAL STUDY; PICHIA KUDRIAVZEVII; PNEUMONIA; PREVOTELLA; PROPIONIBACTERIUM ACNES; PROTEUS MIRABILIS; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SEPSIS; SERRATIA MARCESCENS; STAPHYLOCOCCUS AUREUS; STENOTROPHOMONAS MALTOPHILIA; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; ADULT; AGED; BACTEREMIA; BLOOD; COMPARATIVE STUDY; DIAGNOSTIC TEST; FEMALE; LONGITUDINAL STUDY; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; YOUNG ADULT;

ADULT; AGED; BACTEREMIA; BLOOD CULTURE; DIAGNOSTIC TESTS, ROUTINE; FEMALE; HUMANS; LONGITUDINAL STUDIES; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEPSIS; YOUNG ADULT;

EID: 85014019996     PISSN: 00435325     EISSN: 16137671     Source Type: Journal    
DOI: 10.1007/s00508-017-1181-3     Document Type: Article

References (47)

1. Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med. 2003;348(2):138–50.
2. Martin GS, Mannino DM, Eaton S, Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med. 2003;348(16):1546–54.
3. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, et al. 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Intensive Care Med. 2003;29(4):530–8.
4. Vincent JL, Marshall JC, Namendys-Silva SA, Francois B, Martin-Loeches I, Lipman J, et al. Assessment of the worldwide burden of critical illness: the intensive care over nations (ICON) audit. Lancet Respir Med. 2014;2(5):380–6.
5. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med. 2001;29(7):1303–10.
6. Hall KK, Lyman JA. Updated review of blood culture contamination. Clin Microbiol Rev. 2006;19(4):788–802.
7. Mylotte JM, Tayara A. Blood cultures: clinical aspects and controversies. Eur J Clin Microbiol Infect Dis. 2000;19(3):157–63.
8. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis. 2004;4(12):751–60.
9. Washington JA 2nd, Ilstrup DM. Blood cultures: issues and controversies. Rev Infect Dis. 1986;8(5):792–802.
10. Fraser A, Paul M, Almanasreh N, Tacconelli E, Frank U, Cauda R, et al. Benefit of appropriate empirical antibiotic treatment: thirty-day mortality and duration of hospital stay. Am J Med. 2006;119(11):970–6.
11. Gao F, Melody T, Daniels DF, Giles S, Fox S. The impact of compliance with 6‑hour and 24-hour sepsis bundles on hospital mortality in patients with severe sepsis: a prospective observational study. Crit Care. 2005;9(6):R764–R770.
12. Garnacho-Montero J, Garcia-Garmendia JL, Barrero-Almodovar A, Jimenez-Jimenez FJ, Perez-Paredes C, Ortiz-Leyba C. Impact of adequate empirical antibiotic therapy on the outcome of patients admitted to the intensive care unit with sepsis. Crit Care Med. 2003;31(12):2742–51.
13. Garnacho-Montero J, Ortiz-Leyba C, Herrera-Melero I, Aldabo-Pallas T, Cayuela-Dominguez A, Marquez-Vacaro JA, et al. Mortality and morbidity attributable to inadequate empirical antimicrobial therapy in patients admitted to the ICU with sepsis: a matched cohort study. J Antimicrob Chemother. 2008;61(2):436–41.
14. Harbarth S, Garbino J, Pugin J, Romand JA, Lew D, Pittet D. Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med. 2003;115(7):529–35.
15. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest. 2000;118(1):146–55.
16. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med. 2006;34(6):1589–96.
17. Leibovici L, Shraga I, Drucker M, Konigsberger H, Samra Z, Pitlik SD. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J Intern Med. 1998;244(5):379–86.
18. Paterson DL, Rice LB. Empirical antibiotic choice for the seriously ill patient: are minimization of selection of resistant organisms and maximization of individual outcome mutually exclusive? Clin Infect Dis. 2003;36(8):1006–12.
19. Valles J, Rello J, Ochagavia A, Garnacho J, Alcala MA. Community-acquired bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival. Chest. 2003;123(5):1615–24.
20. Zaragoza R, Artero A, Camarena JJ, Sancho S, Gonzalez R, Nogueira JM. The influence of inadequate empirical antimicrobial treatment on patients with bloodstream infections in an intensive care unit. Clin Microbiol Infect. 2003;9(5):412–8.
21. Struelens MJ, de Mendonca R. The emerging power of molecular diagnostics: towards improved management of life-threatening infection. Intensive Care Med. 2001;27(11):1696–8.
22. Varani S, Stanzani M, Paolucci M, Melchionda F, Castellani G, Nardi L, et al. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect. 2009;58(5):346–51.
23. Avolio M, Diamante P, Zamparo S, Modolo ML, Grosso S, Zigante P, et al. Molecular identification of bloodstream pathogens in patients presenting to the emergency department with suspected sepsis. Shock. 2010;34(1):27–30.
24. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, Salzberger B. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis. 2009;9:126.
25. Figueroa JR, Ortiz J, Morales I. Use of the LightCycler SeptiFast test for rapid etiologic diagnosis of nosocomial infection in gynecological sepsis. Gynecol Obstet Invest. 2010;70(3):215–6.
26. Gaibani P, Rossini G, Ambretti S, Gelsomino F, Pierro AM, Varani S, et al. Blood culture systems: rapid detection – how and why? Int J Antimicrob Agents. 2009;34(Suppl 4):S13–5.
27. Lehmann LE, Hunfeld KP, Steinbrucker M, Brade V, Book M, Seifert H, et al. Improved detection of blood stream pathogens by real-time PCR in severe sepsis. Intensive Care Med. 2010;36(1):49–56.
28. Lodes U, Meyer F, Konig B, Lippert H. Microbiological sepsis screening in surgical ICU patients with the “lightCycler” Septifast test – a pilot study. Zentralbl Chir. 2009;134(3):249–53.
29. Maubon D, Hamidfar-Roy R, Courby S, Vesin A, Maurin M, Pavese P, et al. Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis. J Infect. 2010;61(4):335–42.
30. Mussap M, Molinari MP, Senno E, Gritti P, Soro B, Mannelli S, et al. New diagnostic tools for neonatal sepsis: the role of a real-time polymerase chain reaction for the early detection and identification of bacterial and fungal species in blood samples. J Chemother. 2007;19(Suppl 2):31–4.
31. Tsalik EL, Jones D, Nicholson B, Waring L, Liesenfeld O, Park LP, et al. Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol. 2010;48(1):26–33.
32. Vince A, Lepej SZ, Barsic B, Dusek D, Mitrovic Z, Serventi-Seiwerth R, et al. LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy. J Med Microbiol. 2008;57(Pt 10):1306–7.
33. Wallet F, Nseir S, Baumann L, Herwegh S, Sendid B, Boulo M, et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect. 2010;16(6):774–9.
34. Westh H, Lisby G, Breysse F, Boddinghaus B, Chomarat M, Gant V, et al. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect. 2009;15(6):544–51.
35. Casalta JP, Gouriet F, Roux V, Thuny F, Habib G, Raoult D. Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis. Eur J Clin Microbiol Infect Dis. 2009;28(6):569–73.
36. Mancini N, Clerici D, Diotti R, Perotti M, Ghidoli N, De Marco D, et al. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol. 2008;57(Pt 5):601–4.
37. Steinmann J, Buer J, Rath PM, Paul A, Saner F. Invasive aspergillosis in two liver transplant recipients: diagnosis by SeptiFast. Transpl Infect Dis. 2009;11(2):175–8.
38. von Lilienfeld-Toal M, Lehmann LE, Raadts AD, Hahn-Ast C, Orlopp KS, Marklein G, et al. Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol. 2009;47(8):2405–10.
39. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A, et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol. 2008;197(3):313–24.
40. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ. Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med. 2008;36(5):1487–92.
41. Mencacci A, Leli C, Montagna P, Cardaccia A, Meucci M, Bietolini C, et al. Diagnosis of infective endocarditis: comparison of the LightCycler SeptiFast real-time PCR with blood culture. J Med Microbiol. 2012;61(Pt 6):881–3.
42. Lucignano B, Ranno S, Liesenfeld O, Pizzorno B, Putignani L, Bernaschi P, et al. Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol. 2011;49(6):2252–8.
43. Mongelli G, Romeo MA, Denaro C, Gennaro M, Fraggetta F, Stefani S. Added value of multi-pathogen probe-based real-time PCR SeptiFast in the rapid diagnosis of bloodstream infections in patients with bacteraemia. J Med Microbiol. 2015;64(7):670–5.
44. Suberviola B, Marquez-Lopez A, Castellanos-Ortega A, Fernandez-Mazarrasa C, Santibanez M, Martinez LM. Microbiological diagnosis of sepsis: polymerase chain reaction system versus blood cultures. Am J Crit Care. 2016;25(1):68–75.
45. Calitri C, Denina M, Scolfaro C, Garazzino S, Licciardi F, Burdino E, et al. Etiological diagnosis of bloodstream infections through a multiplex real-time polymerase chain reaction test in pediatric patients: a case series from a tertiary Italian hospital. Infect Dis (Lond). 2015;47(2):73–9.
46. Herne V, Nelovkov A, Kutt M, Ivanova M. Diagnostic performance and therapeutic impact of LightCycler SeptiFast assay in patients with suspected sepsis. Eur J Microbiol Immunol (Bp). 2013;3(1):68–76.
47. Burdino E, Ruggiero T, Allice T, Milia MG, Gregori G, Milano R, et al. Combination of conventional blood cultures and the SeptiFast molecular test in patients with suspected sepsis for the identification of bloodstream pathogens. Diagn Microbiol Infect Dis. 2014;79(3):287–92.