Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis

Clinical Microbiology and Infection

Volumn 15, Issue 6, 2009, Pages 544-551

  Westh, Henrik ^a   Lisby, G ^a   Breysse, F ^b   Boddinghaus B ^c   Chomarat, M ^b   Gant, V ^d   Goglio, A ^e   Raglio, A ^e   Schuster, H ^d   Stuber, F ^f   Wissing, H ^c   Hoeft, A ^f  

^a HVIDOVRE HOSPITAL   (Denmark)

^b CENTRE HOSPITALIER LYON SUD   (France)

^c GOETHE UNIVERSITY   (Germany)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

^e OSPEDALI RIUNITI   (Italy)

^f UNIVERSITY HOSPITAL BONN   (Germany)

Author keywords

Blood culture; LightCycler; Multiplex; PCR; Sepsis; SeptiFast

Indexed keywords

ANTIINFECTIVE AGENT;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; BLOODSTREAM INFECTION; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC VALUE; DRUG EFFICACY; FUNGAL CONTAMINATION; FUNGEMIA; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; HUMAN; MAJOR CLINICAL STUDY; MULTICENTER STUDY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEPSIS; SPECIES COMPARISON;

BACTERIA (MICROORGANISMS);

EID: 67651049965     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2009.02736.x     Document Type: Article

References (43)

1. Martin GS, Mannino DM, Eaton S, Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003; 348: 1546-1554.
2. Biedenbach DJ, Moet GJ, Jones RN. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn Microbiol Infect Dis 2004; 50: 59-69.
3. Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: Analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 2004; 39: 309-317.
4. Garnacho-Montero J, Garcia-Garmendia JL, Barrero-Almodovar A, Jimenez-Jimenez FJ, Perez-Paredes C, Ortiz-Leyba C. Impact of adequate empirical antibiotic therapy on the outcome of patients admitted to the intensive care unit with sepsis. Crit Care Med 2003; 31: 2742-2751.
5. Zaragoza R, Artero A, Camarena JJ, Sancho S, Gonzalez R, Nogueira JM. The influence of inadequate empirical antimicrobial treatment on patients with bloodstream infections in an intensive care unit. Clin Microbiol Infect 2003; 9: 412-418.
6. Leibovici L, Shraga I, Drucker M, Konigsberger H, Samra Z, Pitlik SD. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J Intern Med 1998; 244: 379-386.
7. Valles J, Rello J, Ochagavia A, Garnacho J, Alcala MA. Community-acquired bloodstream infection in critically ill adult patients: Impact of shock and inappropriate antibiotic therapy on survival. Chest 2003; 123: 1615-1624.
8. Magadia RR, Weinstein MP. Laboratory diagnosis of bacteremia and fungemia. Infect Dis Clin North Am 2001; 15: 1009-1024.
9. Cockerill FR 3rd, Wilson JW, Vetter EA et al. Optimal testing parameters for blood cultures. Clin Infect Dis 2004; 38: 1724-1730.
10. Members of the American College of Chest Physicians. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Crit Care Med 1992; 20: 864-874.
11. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA 1995; 273: 117-123.
12. Sands KE, Bates DW, Lanken PN et al. Epidemiology of sepsis syndrome in 8 academic medical centers. Academic Medical Center Consortium Sepsis Project Working Group. JAMA 1997; 278: 234-240.
13. Iralu JV, Sritharan VK, Pieciak WS, Wirth DF, Maguire JH, Barker RH Jr. Diagnosis of Mycobacterium avium bacteremia by polymerase chain reaction. J Clin Microbiol 1993; 31: 1811-1814.
14. Kami M, Fukui T, Ogawa S et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 2001; 33: 1504-1512.
15. Maaroufi Y, Heymans C, De Bruyne JM et al. Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J Clin Microbiol 2003; 41: 3293-3298.
16. Song JH, Cho H, Park MY, Na DS, Moon HB, Pai CH. Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction. J Clin Microbiol 1993; 31: 1439-1443.
17. Rothman RE, Majmudar MD, Kelen GD et al. Detection of bacteremia in emergency department patients at risk for infective endocarditis using universal 16S rRNA primers in a decontaminated polymerase chain reaction assay. J Infect Dis 2002; 186: 1677-1681.
18. Klaschik S, Lehmann LE, Raadts A, Hoeft A, Stuber F. Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR. Mol Biotechnol 2002; 22: 231-242.
19. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Fox AJ, Kaczmarski EB. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol 2001; 39: 1553-1558.
20. Klaschik S, Lehmann LE, Raadts A et al. Detection and differentiation of in.vitro-spiked bacteria by real-time PCR and melting-curve analysis. J Clin Microbiol 2004; 42: 512-517.
21. Garcia-Erce JA, Grasa JM, Solano VM et al. Bacterial contamination of blood components due to Burkholderia cepacia contamination from clorhexidine bottles. Vox Sang 2002; 83: 70-71.
22. Oie S, Kamiya A. Microbial contamination of antiseptics and disinfectants. Am J Infect Control 1996; 24: 389-395.
23. Dave J, Springbett R, Padmore H, Turner P, Smith G. Pseudomonas cepacia pseudobacteraemia. J Hosp Infect 1993; 23: 72-73.
24. Peters RP, Mohammadi T, Vandenbroucke-Grauls CM, Danner SA, van Agtmael MA, Savelkoul PH. Detection of bacterial DNA in blood samples from febrile patients: Underestimated infection or emerging contamination? FEMS Immunol Med Microbiol 2004; 42: 249-253.
25. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Kaczmarski EB, Fox AJ. Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol 2000; 38: 1747-1752.
26. Mohammadi T, Reesink HW, Vandenbroucke-Grauls CM, Savelkoul PH. Optimization of realtime PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates. J Clin Microbiol 2003; 41: 4796-4798.
27. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 2004; 4: 751-760.
28. Mancini N, Clerici D, Diotti R et al. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 2008; 57: 601-604.
29. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ. Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med 2008; 36: 1487-1492.
30. Roche Molecular Systems. LightCycler® SeptiFast Test Mgrade. For use with the LightCycler® 2.0 Instrument (serial number 1415001 and higher). 04623886001 ed. 2005.
31. Iwen PC, Hinrichs SH, Rupp ME. Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med Mycol 2002; 40: 87-109.
32. Weinstein MP. Blood culture contamination: Persisting problems and partial progress. J Clin Microbiol 2003; 41: 2275-2278.
33. Weinstein MP, Towns ML, Quartey SM et al. The clinical significance of positive blood cultures in the 1990s: A prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 1997; 24: 584-602.
34. Reimer LG, Wilson ML, Weinstein MP. Update on detection of bacteremia and fungemia. Clin Microbiol Rev 1997; 10: 444-465.
35. Richter SS, Beekmann SE, Croco JL et al. Minimizing the workup of blood culture contaminants: Implementation and evaluation of a laboratory-based algorithm. J Clin Microbiol 2002; 40: 2437-2444.
36. Hall KK, Lyman JA. Updated review of blood culture contamination. Clin Microbiol Rev 2006; 19: 788-802.
37. Fischer JE, Bachmann LM, Jaeschke R. A readers' guide to the interpretation of diagnostic test properties: Clinical example of sepsis. Intensive Care Med 2003; 29: 1043-1051.
38. Hope WW, Walsh TJ, Denning DW. Laboratory diagnosis of invasive aspergillosis. Lancet Infect Dis 2005; 5: 609-622.
39. Klont RR, Meis JF, Verweij PE. Critical assessment of issues in the diagnosis of invasive aspergillosis. Clin Microbiol Infect 2001; 7 (suppl 2): 32-37.
40. Burkardt HJ. Standardization and quality control of PCR analyses. Clin Chem Lab Med 2000; 38: 87-91.
41. Fraser A, Paul M, Almanasreh N et al. Benefit of appropriate empirical antibiotic treatment: Thirty-day mortality and duration of hospital stay. Am J Med 2006; 119: 970-976.
42. Vetter E, Torgerson C, Feuker A et al. Comparison of the BACTEC MYCO/F lytic bottle to the isolator tube, BACTEC plus aerobic F/bottle, and BACTEC anaerobic lytic/10 bottle and comparison of the BACTEC plus aerobic F/bottle to the isolator tube for recovery of bacteria, mycobacteria, and fungi from blood. J Clin Microbiol 2001; 39: 4380-4386.
43. Hadgu A, Dendukuri N, Hilden J. Evaluation of nucleic acid amplification tests in the absence of a perfect gold-standard test: A review of the statistical and epidemiologic issues. Epidemiology 2005; 16: 604-612.