Combination of conventional blood cultures and the SeptiFast molecular test in patients with suspected sepsis for the identification of bloodstream pathogens

Diagnostic Microbiology and Infectious Disease

Volumn 79, Issue 3, 2014, Pages 287-292

  Burdino, Elisa ^a   Ruggiero, Tina ^a   Allice, Tiziano ^a   Milia, Maria Grazia ^a   Gregori, Gabriella ^a   Milano, Rosangela ^a   Cerutti, Francesco ^a   De Rosa, Francesco Giuseppe ^b   Manno, Emilpaolo ^c   Caramello, Pietro ^b   Di Perri, Giovanni ^b   Ghisetti, Valeria ^a  

^a AMEDEO DI SAVOIA HOSPITAL   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

^c MARIA VITTORIA HOSPITAL   (Italy)

Author keywords

Blood culture; Bloodstream infection; Sepsis; SeptiFast

Indexed keywords

ANTIBIOTIC AGENT;

ADULT; ANTIBIOTIC THERAPY; ARTICLE; ASPERGILLUS FUMIGATUS; BLOOD CULTURE; BLOODSTREAM INFECTION; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA KRUSEI; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; IMMUNOCOMPROMISED PATIENT; INTERMETHOD COMPARISON; ITALY; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MAJOR CLINICAL STUDY; MICROORGANISM DETECTION; NONHUMAN; NUCLEIC ACID ANALYSIS; PREVALENCE; PRIORITY JOURNAL; PROSPECTIVE STUDY; PSEUDOMONAS AERUGINOSA; SEPSIS; SEPTIFAST TEST; SERRATIA MARCESCENS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS HAEMOLYTICUS; STENOTROPHOMONAS MALTOPHILIA; STREPTOCOCCUS PNEUMONIAE; BACTERIA; BLOOD; CLASSIFICATION; EVALUATION STUDY; FUNGUS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PROCEDURES; SENSITIVITY AND SPECIFICITY;

ADULT; BACTERIA; BLOOD; FUNGI; HUMANS; MICROBIOLOGICAL TECHNIQUES; MOLECULAR DIAGNOSTIC TECHNIQUES; SENSITIVITY AND SPECIFICITY; SEPSIS;

EID: 84902074037     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2014.03.018     Document Type: Article

References (31)

1. Casalta J.P., Gouriet F., Roux V., Thuny F., Habib G., Raoult D. Evaluation of the LightCyclerSeptiFast test in the rapid etiologic diagnostic of infectious endocarditis. Eur J Clin Microbiol Infect Dis 2009, 28:569-573.
2. Chang S.-S., Hsieh W.-H., Liu T.-S., Lee S.-H., Wang C.-H., Chou H.-C., et al. Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis-a systemic review and meta-analysis. PLoS One 2013, 8:e62323. I.P. Androulakis (Ed.).
3. Cuenca-Estrella M., Meije Y., Diaz-Pedroche C., Gomez-Lopez A., Buitrago M.J., Bernal-Martinez L., et al. Value of serial quantification of fungal DNA by a real-time PCR-based technique for early diagnosis of invasive aspergillosis in patients with febrile neutropenia. J Clin Microbiol 2009, 47:379-384.
4. Cunha B.A. Sepsis and septic shock: selection of empiric antimicrobial therapy. Crit Care Clin 2008, 24:313-334.
5. Dierkes C., Ehrenstein B., Siebig S., Linde H.-J., Reischl U., Salzberger B. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 2009, 9:126.
6. Fernández-Cruz A., Marín M., Kestler M., Alcalá L., Rodriguez-Créixems M., Bouza E. The value of combining blood culture and SeptiFast data for predicting complicated bloodstream infections caused by Gram-positive bacteria or Candida species. J Clin Microbiol 2013, 51:1130-1136.
7. Harbarth S., Garbino J., Pugin J., Romand J.A., Lew D., Pittet D. Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med 2003, 115:529-535.
8. Huang L., Quartin A., Jones D., Havlir D.V. Intensive care of patients with HIV infection. N Engl J Med 2006, 355:173-181.
9. Klein Klouwenberg P.M.C., Ong D.S.Y., Bonten M.J.M., Cremer O.L. Classification of sepsis, severe sepsis and septic shock: the impact of minor variations in data capture and definition of SIRS criteria. Intensive Care Med 2012, 38:811-819.
10. Lehmann L.E., Hunfeld K.-P., Emrich T., Haberhausen G., Wissing H., Hoeft A., et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 2008, 197:313-324.
11. Leyssene D., Gardes S., Vilquin P., Flandrois J.-P., Carret G., Lamy B. Species-driven interpretation guidelines in case of a single-sampling strategy for blood culture. Eur J Clin Microbiol Infect Dis 2011, 30:1537-1541.
12. Lucignano B., Ranno S., Liesenfeld O., Pizzorno B., Putignani L., Bernaschi P., et al. Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol 2011, 49:2252-2258.
13. Mancini N., Clerici D., Diotti R., Perotti M., Ghidoli N., De Marco D., et al. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 2008, 57:601-604.
14. Mancini N., Carletti S., Ghidoli N., Cichero P., Ossi C.M., Ieri R., et al. Molecular diagnosis of polymicrobial sepsis. J Clin Microbiol 2009, 47:1274-1275.
15. Mancini N., Carletti S., Ghidoli N., Cichero P., Burioni R., Clementi M. The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev 2010, 23:235-251.
16. Mauro M.V., Cavalcanti P., Perugini D., Noto A., Sperlì D., Giraldi C. Diagnostic utility of LightCyclerSeptiFast and procalcitonin assays in the diagnosis of bloodstream infection in immunocompromised patients. Diagn Microbiol Infect Dis 2012, 73:308-311.
17. Mussap M., Molinari M.P., Senno E., Gritti P., Soro B., Mannelli S., et al. New diagnostic tools for neonatal sepsis: the role of a real-time polymerase chain reaction for the early detection and identification of bacterial and fungal species in blood samples. J Chemother 2007, 19(Suppl 2):31-34.
18. Paolucci M., Landini M.P., Sambri V. Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents 2010, 36(Suppl 2):S6-S16.
19. Paolucci M., Stanzani M., Melchionda F., Tolomelli G., Castellani G., Landini M.P., et al. Routine use of a real-time polymerase chain reaction method for detection of bloodstream infections in neutropaenic patients. Diagn Microbiol Infect Dis 2013, 75:130-134.
20. Pasqualini L., Mencacci A., Leli C., Montagna P., Cardaccia A., Cenci E., et al. Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward. J Clin Microbiol 2012, 50:1285-1288.
21. Sharma S., Kumar A. Antimicrobial management of sepsis and septic shock. Clin Chest Med 2008, 29:677-687.
22. Steinmann J., Buer J., Rath P.-M., Paul A., Saner F. Invasive aspergillosis in two liver transplant recipients: diagnosis by SeptiFast. Transpl Infect Dis 2009, 11:175-178.
23. Varani S., Stanzani M., Paolucci M., Melchionda F., Castellani G., Nardi L., et al. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect 2009, 58:346-351.
24. Vince A., Lepej S.Z., Barsić B., Dusek D., Mitrović Z., Serventi-Seiwerth R., et al. LightCyclerSeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy. J Med Microbiol 2008, 57:1306-1307.
25. Von Lilienfeld-Toal M., Lehmann L.E., Raadts A.D., Hahn-Ast C., Orlopp K.S., Marklein G., et al. Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 2009, 47:2405-2410.
26. Wallet F., Nseir S., Baumann L., Herwegh S., Sendid B., Boulo M., et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect 2010, 16:774-779.
27. Weinstein M.P. Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 2003, 41:2275-2278.
28. Weinstein M.P., Towns M.L., Quartey S.M., Mirrett S., Reimer L.G., Parmigiani G., et al. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 1997, 24:584-602.
29. Westh H., Lisby G., Breysse F., Böddinghaus B., Chomarat M., Gant V., et al. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 2009, 15:544-551.
30. Wilson M.L., Mitchell M., Murray P., Reiner L.G., Reller L.B., Towns S., et al. Principles and procedures for blood cultures: approved guidelines 2007, Clinical Laboratory Standards Institute, Wayne, PA.
31. Yanagihara K., Kitagawa Y., Tomonaga M., Tsukasaki K., Kohno S., Seki M., et al. Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care 2010, 14:R159.