Single-molecule FRET supports the two-state model of argonaute action

RNA Biology

Volumn 11, Issue 1, 2014, Pages 45-56

  Zander, Adrian ^a   Holzmeister, Phil ^a   Klose, Daniel ^b   Tinnefeld, Philip ^a   Grohmann, Dina ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

Amber suppressor strategy; Archaea; Argonaute; Biophysics; Dynamics; Fluorescence; FRET; Single molecule fluorescence spectroscopy

Indexed keywords

ARGONAUTE PROTEIN; DOUBLE STRANDED DNA; FLUORESCENT DYE; SINGLE STRANDED DNA;

AMINO TERMINAL SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COVALENT BOND; DNA CLEAVAGE; DNA DNA HYBRIDIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS; IN VITRO STUDY; METHANOCALDOCOCCUS JANNASCHII; MIDDLE DOMAIN; NONHUMAN; PAZ DOMAIN; PIWI DOMAIN; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN STRUCTURE; SINGLE MOLECULE FLUORESCENCE RESONANCE ENERGY TRANSFER;

AMBER SUPPRESSOR STRATEGY; ARCHAEA; ARGONAUTE; BIOPHYSICS; DYNAMICS; FLUORESCENCE; FRET; SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPY;

ARGONAUTE PROTEINS; CLONING, MOLECULAR; DNA; FLUORESCENCE RESONANCE ENERGY TRANSFER; METHANOCALDOCOCCUS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 85011940616     PISSN: 15476286     EISSN: 15558584     Source Type: Journal    
DOI: 10.4161/rna.27446     Document Type: Article

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