Human intervention study to assess the effects of supplementation with olive leaf extract on peripheral blood mononuclear cell gene expression

International Journal of Molecular Sciences

Volumn 17, Issue 12, 2016, Pages

  Boss, Anna ^a   Kao, Chi Hsiu Juei ^a,b   Murray, Pamela M ^b   Marlow, Gareth ^c   Barnett, Matthew P G ^d   Ferguson, Lynnette R ^a,b  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b THE UNIVERSITY OF AUCKLAND   (New Zealand)

^c UNIVERSITY HOSPITAL OF WALES   (United Kingdom)

^d AGRESEARCH GRASSLANDS   (New Zealand)

Author keywords

Inflammation; Nutraceutical; Polyphenols; Transcriptomics

Indexed keywords

ANTIINFLAMMATORY AGENT; CYCLOOXYGENASE 2 INHIBITOR; EARLY GROWTH RESPONSE FACTOR 1; EARLY GROWTH RESPONSE FACTOR 2; HEPARIN BINDING EPIDERMAL GROWTH FACTOR; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INHIBITOR OF DIFFERENTIATION 3; INTERLEUKIN 8; KRUPPEL LIKE FACTOR; MACROPHAGE MIGRATION INHIBITION FACTOR; MESODERMAL GROWTH FACTOR; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; NDC80 COMPLEX; NUCLEAR RECEPTOR RELATED FACTOR 1; NUCLEOSOME ASSEMBLY PROTEIN 1; OLIVE LEAF EXTRACT; ONCOSTATIN M; PLACEBO; PLANT EXTRACT; POLYPHENOL; PROSTAGLANDIN SYNTHASE; UNCLASSIFIED DRUG; MESSENGER RNA;

ANTIINFLAMMATORY ACTIVITY; ARTICLE; CYCLOOXYGENASE 2 GENE; DOUBLE BLIND PROCEDURE; EARLY GROWTH RESPONSE 1 GENE; ENZYME INDUCTION; GENE; GENE EXPRESSION; GENE ONTOLOGY; HUMAN; INHIBITOR OF DIFFERENTIATION 3 GENE; INTERVENTION STUDY; LIPID METABOLISM; MALE; MICROARRAY ANALYSIS; NUTRITIONAL ASSESSMENT; OLIVE; PATIENT COMPLIANCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN ANALYSIS; QUALITY CONTROL; RANDOMIZED CONTROLLED TRIAL; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TREATMENT OUTCOME; CHEMISTRY; CONTROLLED STUDY; DIETARY SUPPLEMENT; DNA MICROARRAY; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; INNATE IMMUNITY; METABOLISM; MOLECULAR GENETICS; MONONUCLEAR CELL; OLIVE TREE; PLANT LEAF; REPRODUCIBILITY;

DIETARY SUPPLEMENTS; GENE EXPRESSION REGULATION; GENE ONTOLOGY; HUMANS; IMMUNITY, INNATE; LEUKOCYTES, MONONUCLEAR; MALE; MOLECULAR SEQUENCE ANNOTATION; OLEA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PLACEBOS; PLANT EXTRACTS; PLANT LEAVES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 85003819834     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms17122019     Document Type: Article

References (84)

1. Tunca, B., Tezcan, G., Cecener, G., Egeli, U., Ak, S., Malyer, HI., Tumen, G, Bilir, A. Olea europaea leaf extract alters microRNA expression in human glioblastoma eells. J. Cancer Res. Clin. Oncol. 2012,138,1831-1844:. [CrossRef] [PubMed]
2. Samet, I., Han, J., Jlaiel, L., Sayadi, S., Isoda, H. Olive (Olea europaea) Leaf Extract Induces Apoptosis and Monocyte/Macrophage Differentiation in Human Chronic Myelogenous Leukemia K562 Cells: Insight into the Underlying Mechanism. Oxid. Med. Cell. Longev. 2014, 2014, 927619. [CrossRef] [PubMed]
3. Ryu, S.-J., Choi, H.-S., Yoon, K.-Y., Lee, O.-H., Kim, K.-J., Lee, B.-Y. Oleuropein suppresses LPS-induced inflammatory responses in RAW264.7 cell and zebrafish. J. Agric. Food Chem. 2015, 63, 2098-2105. [CrossRef] [PubMed]
4. Shen, Y., Song, S.J., Keum, N., Park, T. Olive Leaf Extract Attenuates Obesity in High-Fat Diet-Fed Mice by Modulating the Expression of Molecules Involved in Adipogenesis and Thermogenesis. Evid. Based Complement. Altern. Med. 2014, 2014, 971890. [CrossRef] [PubMed]
5. Liu, Y.-N., Jung, J.-H., Park, H., Kim, H. Olive leaf extract suppresses messenger RNA expression of proinflammatory cytokines and enhances insulin receptor substrate 1 expression in the rats with streptozotocin and high-fat diet-Induced diabetes. Nutr. Res. 2014. [CrossRef] [PubMed]
6. Bayram, B., Ozcelik, B., Grimm, S., Roeder, T., Schrader, C., Ernst, I.M.A., Wagner, A.E., Grune, T., Frank, J., Rimbach, G. A diet rich in olive oil phenolics reduces oxidative stress in the heart of SAMP8 mice by induction of Nrf2-dependent gene expression. Rejuvenation Res. 2012, 15, 71-81. [CrossRef] [PubMed]
7. Paredes-Gonzalez, X., Fuentes, F., Jeffery, S., Saw, C.L.-L., Shu, L., Su, Z.-Y., Kong, A.-N.T. Induction of NRF2-mediated gene expression by dietary phytochemical flavones apigenin and luteolin. Biopharm. Drug Dispos. 2015, 36, 440-451. [CrossRef] [PubMed]
8. Crespo, M.C., Tomé-Carneiro, J., Burgos-Ramos, E., Loria Kohen, V., Espinosa, M.I., Herranz, J., Visioli, F. One-week administration of hydroxytyrosol to humans does not activate Phase II enzymes. Pharmacol. Res. 2015, 95-96, 132-137. [CrossRef] [PubMed]
9. Lockyer, S., Rowland, I., Spencer, J.P.E., Yaqoob, P., Stonehouse, W. Impact of phenolic-rich olive leaf extract on blood pressure, plasma lipids and inflammatory markers: A randomised controlled trial. Eur. J. Nutr. 2016. [CrossRef] [PubMed]
10. De Bock, M., Derraik, J.G.B., Brennan, C.M., Biggs, J.B., Morgan, P.E., Hodgkinson, S.C., Hofman, P.L., Cutfield, W.S. Olive (Olea europaea L.) leaf polyphenols improve insulin sensitivity in middle-aged overweight men: A randomized, placebo-controlled, crossover trial. PLoS ONE 2013, 8, e57622. [CrossRef] [PubMed]
11. Visioli, F. Can Experimental Pharmacology Be Always Applied to Human Nutrition? Int. J. Food Sci. Nutr. 2015. Available online: http://www.tandfonline.com/doi/full/10.3109/09637486.2012.665439#abstract (accessed on 12 August 2015). [CrossRef] [PubMed]
12. Affymetrix, Affymetrix® Expression Console™ Software 1.4.1. 2015. Available online: http://www.affymetrix.com/estore/browse/level_seven_software_products_only.jsp?productId=131414#1_1 (accessed on 19 February 2016).
13. Benjamin, Y., Hochberg, Y. Controlling the false discovery rate: A practical and powerful approach to multiple testing. J. R. Stat. Soc. Ser. B 1995, 57, 289-300.
14. Elliott, R.M., de Roos, B., Duthie, S.J., Bouwman, F.G., Rubio-Aliaga, I., Crosley, L.K., Mayer, C., Polley, A.C., Heim, C., Coort, S.L., et al. Transcriptome analysis of peripheral blood mononuclear cells in human subjects following a 36 h fast provides evidence of effects on genes regulating inflammation, apoptosis and energy metabolism. Genes Nutr. 2014, 9, 432. [CrossRef] [PubMed]
15. Bouwens, M., Afman, L.A., Müller, M. Fasting Induces Changes in Peripheral Blood Mononuclear Cell Gene Expression Profiles Related to Increases in Fatty Acid Beta-Oxidation: Functional Role of Peroxisome Proliferator Activated Receptor Alpha in Human Peripheral Blood Mononuclear Cells. Am. J. Clin. Nutr. 2007, 86, 1515-1523. [PubMed]
16. Konstantinidou, V., Khymenets, O., Covas, M.-I., de la Torre, R., Muñoz-Aguayo, D., Anglada, R., Farré, M., Fito, M. Time course of changes in the expression of insulin sensitivity-related genes after an acute load of virgin olive oil. OMICS 2009, 13, 431-438. [CrossRef] [PubMed]
17. Ashburner, M., Ball, C.A., Blake, J.A., Botstein, D., Butler, H., Cherry, J.M., Davis, A.P., Dolinski, K., Dwight, S.S., Eppig, J.T., et al. Gene ontology: Tool for the unification of biology. The Gene Ontology Consortium. Nat. Genet. 2000, 25, 25-29. [CrossRef] [PubMed]
18. The Gene Ontology Consortium. Gene Ontology Consortium: Going forward. Nucleic Acids Res. 2015, 43, D1049-D1056.
19. Chang, J.T., Nevins, J.R. GATHER: A systems approach to interpreting genomic signatures. Bioinformatics 2006, 22, 2926-2933. [CrossRef] [PubMed]
20. Mi, H., Dong, Q., Muruganujan, A., Gaudet, P., Lewis, S., Thomas, P.D. PANTHER version 7: Improved phylogenetic trees, orthologs and collaboration with the Gene Ontology Consortium. Nucleic Acids Res. 2010, 38, D204-D210. [CrossRef] [PubMed]
21. QIAGEN, Redwood City, U. Ingenuity Pathway Analysis® (2015). Available online: http://www.qiagen. com/ingenuity (accessed on 24 February 2016).
22. Fontana, M.F., Baccarella, A., Pancholi, N., Pufall, M.A., Herbert, D.R., Kim, C.C. JUNB is a key transcriptional modulator of macrophage activation. J. Immunol. 2015, 194, 177-186. [CrossRef] [PubMed]
23. Olsen, K.S., Skeie, G., Lund, E. Whole-Blood Gene Expression Profiles in Large-Scale Epidemiological Studies: What Do They Tell? Curr. Nutr. Rep. 2015, 4, 377-386. [CrossRef] [PubMed]
24. Liew, C.-C., Ma, J., Tang, H.-C., Zheng, R., Dempsey, A.A. The peripheral blood transcriptome dynamically reflects system wide biology: A potential diagnostic tool. J. Lab. Clin. Med. 2006, 147, 126-132. [CrossRef] [PubMed]
25. Hanekamp, J.C., Bast, A., Calabrese, E.J. Nutrition and health—Transforming research traditions. Crit. Rev. Food Sci. Nutr. 2015, 55, 1074-1080. [CrossRef] [PubMed]
26. Kapoor, D., Trikha, D., Vijayvergiya, R., Kaul, D., Dhawan, V. Conventional therapies fail to target inflammation and immune imbalance in subjects with stable coronary artery disease: A system-based approach. Atherosclerosis 2014, 237, 623-631. [CrossRef] [PubMed]
27. Beauchamp, G.K., Keast, R.S.J., Morel, D., Lin, J., Pika, J., Han, Q., Lee, C.-H., Smith, A.B., Breslin, P.A.S. Phytochemistry: Ibuprofen-like activity in extra-virgin olive oil. Nature 2005, 437, 45-46. [CrossRef] [PubMed]
28. Rosignoli, P., Fuccelli, R., Fabiani, R., Servili, M., Morozzi, G. Effect of olive oil phenols on the production of inflammatory mediators in freshly isolated human monocytes. J. Nutr. Biochem. 2013, 24, 1513-1519. [CrossRef] [PubMed]
29. Scoditti, E., Calabriso, N., Massaro, M., Pellegrino, M., Storelli, C., Martines, G., de Caterina, R., Carluccio, M.A. Mediterranean diet polyphenols reduce inflammatory angiogenesis through MMP-9 and COX-2 inhibition in human vascular endothelial cells: A potentially protective mechanism in atherosclerotic vascular disease and cancer. Arch. Biochem. Biophys. 2012, 527, 81-89. [CrossRef] [PubMed]
30. Sánchez-Fidalgo, S., Cárdeno, A., Sánchez-Hidalgo, M., Aparicio-Soto, M., de la Lastra, C.A. Dietary extra virgin olive oil polyphenols supplementation modulates DSS-induced chronic colitis in mice. J. Nutr. Biochem. 2013, 24, 1401-1413. [CrossRef] [PubMed]
31. Silva, S., Sepodes, B., Rocha, J., Direito, R., Fernandes, A., Brites, D., Freitas, M., Fernandes, E., Bronze, M.R., Figueira, M.E. Protective effects of hydroxytyrosol-supplemented refined olive oil in animal models of acute inflammation and rheumatoid arthritis. J. Nutr. Biochem. 2015, 26, 360-368. [CrossRef] [PubMed]
32. Lockyer, S., Corona, G., Yaqoob, P., Spencer, J.P.E., Rowland, I. Secoiridoids delivered as olive leaf extract induce acute improvements in human vascular function and reduction of an inflammatory cytokine: A randomised, double-blind, placebo-controlled, cross-over trial. Br. J. Nutr. 2015, 1-9. [CrossRef] [PubMed]
33. Waugh, D.J.J., Wilson, C. The interleukin-8 pathway in cancer. Clin. Cancer Res. 2008, 14, 6735-6741. [CrossRef] [PubMed]
34. KB expression and activity upon differentiation of human embryonic stem cell line SNUhES3. Stem Cells Dev. 2007, 16, 615-623. [CrossRef] [PubMed]
35. Schmid, D.I., Schwertz, H., Jiang, H., Campbell, R.A., Weyrich, A.S., McIntyre, T.M., Zimmerman, G.A., Kraiss, L.W. Translational control of JunB, an AP-1 transcription factor, in activated human endothelial cells. J. Cell. Biochem. 2013, 114, 1519-1528. [CrossRef] [PubMed]
36. Gomard, T., Michaud, H.-A., Tempé, D., Thiolon, K., Pelegrin, M., Piechaczyk, M. An NF-kappaB-dependent role for JunB in the induction of proinflammatory cytokines in LPS-activated bone marrow-derived dendritic cells. PLoS ONE 2010, 5, e9585. [CrossRef] [PubMed]
37. Miyata, K., Yotsumoto, F., Nam, S.O., Kuroki, M., Miyamoto, S. Regulatory mechanisms of the HB-EGF autocrine loop in inflammation, homeostasis, development and cancer. Anticancer Res. 2012, 32, 2347-2352. [PubMed]
38. Yotsumoto, F., Yagi, H., Suzuki, S.O., Oki, E., Tsujioka, H., Hachisuga, T., Sonoda, K., Kawarabayashi, T., Mekada, E., Miyamoto, S. Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem. Biophys. Res. Commun. 2008, 365, 555-561. [CrossRef] [PubMed]
39. Pan, Z., Wang, Z., Yang, H., Zhang, F., Reinach, P.S. TRPV1 activation is required for hypertonicity-stimulated inflammatory cytokine release in human corneal epithelial cells. Investig. Ophthalmol. Vis. Sci. 2011, 52, 485-493. [CrossRef] [PubMed]
40. Tawara, K., Bolin, C., Koncinsky, J., Jorcyk, C.L. Abstract 2276: Oncostatin M promotes breast cancer metastasis to lung by affecting initial stages of metastasis. Cancer Res. 2015. [CrossRef]
41. Covert, H., Mellor, L. Abstract 5198: Contribution of inflammatory cytokines to CD44-mediated breast cancer metastatic potential. Cancer Res. 2015. [CrossRef]
42. Zhu, M., Che, Q., Liao, Y., Wang, H., Wang, J., Chen, Z., Wang, F., Dai, C., Wan, X. Oncostatin M Activates STAT3 to Promote Endometrial Cancer Invasion and Angiogenesis. Oncol. Rep. 2015, 34, 129-138. Available online: http://www.spandidos-publications.com/or/34/1/129/abstract (accessed on 19 January 2016). [CrossRef] [PubMed]
43. Pöling, J., Gajawada, P., Richter, M., Lörchner, H., Polyakova, V., Kostin, S., Shin, J., Boettger, T., Walther, T., Rees, W., et al. Therapeutic targeting of the oncostatin M receptor-β prevents inflammatory heart failure. Basic Res. Cardiol. 2014. [CrossRef] [PubMed]
44. Pahl, H.L. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene 1999, 18, 6853-6866. [CrossRef] [PubMed]
45. Ghosh, S., May, M.J., Kopp, E.B. NF-kappa B and Rel proteins: Evolutionarily conserved mediators of immune responses. Annu. Rev. Immunol. 1998, 16, 225-260. [CrossRef] [PubMed]
46. KB activation. Cold Spring Harb. Perspect. Biol. 2010, 2, a000158. [CrossRef] [PubMed]
47. KB. Genes Dev. 2004, 18, 2195-2224. [CrossRef] [PubMed]
48. KB activation. Biochem. Biophys. Res. Commun. 2003, 310, 720-724. [CrossRef] [PubMed]
49. Rhee, S.G. Regulation of phosphoinositide-specific phospholipase C. Annu. Rev. Biochem. 2001, 70, 281-312. [CrossRef] [PubMed]
50. Follo, M.Y., Manzoli, L., Poli, A., McCubrey, J.A., Cocco, L. PLC and PI3K/Akt/mTOR signalling in disease and cancer. Adv. Biol. Regul. 2015, 57, 10-16. [CrossRef] [PubMed]
51. Murakami, M., Kudo, I. Phospholipase A2. J. Biochem. 2002, 131, 285-292. Available online: http://www. ncbi.nlm.nih.gov/pubmed/11872155 (accessed on 29 February 2016). [CrossRef] [PubMed]
52. Niu, L., He, X.-H., Wang, Q.-W., Fu, M.-Y., Xu, F., Xue, Y., Wang, Z.-Z., An, X.-J. Polyphenols in Regulation of Redox Signaling and Inflammation During Cardiovascular Diseases. Cell Biochem. Biophys. 2015. [CrossRef] [PubMed]
53. Liu, B., Qu, L., Yan, S. Cyclooxygenase-2 promotes tumor growth and suppresses tumor immunity. Cancer Cell Int. 2015, 15, 106. [CrossRef] [PubMed]
54. KB. Eur. Rev. Med. Pharmacol. Sci. 2014, 18, 1163-1169. [PubMed]
55. Bonnefont-Rousselot, D. Lp-PLA2, a biomarker of vascular inflammation and vulnerability of atherosclerosis plaques. Ann. Pharm. Fr. 2015. [CrossRef]
56. Masoodi, M., Kuda, O., Rossmeisl, M., Flachs, P., Kopecky, J. Lipid signaling in adipose tissue: Connecting inflammation & metabolism. Biochim. Biophys. Acta 2015, 1851, 503-518. [PubMed]
57. Yang, X., Lu, X., Lombès, M., Rha, G.B., Chi, Y.-I., Guerin, T.M., Smart, E.J., Liu, J. The G(0)/G(1) switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase. Cell Metab. 2010, 11, 194-205. [CrossRef] [PubMed]
58. Ma, T., Lopez-Aguiar, A.G.N., Li, A., Lu, Y., Sekula, D., Nattie, E.E., Freemantle, S., Dmitrovsky, E. Mice lacking G0S2 are lean and cold-tolerant. Cancer Biol. Ther. 2014, 15, 643-650. [CrossRef] [PubMed]
59. Zhang, X., Xie, X., Heckmann, B.L., Saarinen, A.M., Czyzyk, T.A., Liu, J. Targeted disruption of G0/G1 switch gene 2 enhances adipose lipolysis, alters hepatic energy balance, and alleviates high-fat diet-induced liver steatosis. Diabetes 2014, 63, 934-946. [CrossRef] [PubMed]
60. El-Assaad, W., El-Kouhen, K., Mohammad, A.H., Yang, J., Morita, M., Gamache, I., Mamer, O., Avizonis, D., Hermance, N., Kersten, S., et al. Deletion of the gene encoding G0/G1 switch protein 2 (G0s2) alleviates high-fat-diet-induced weight gain and insulin resistance, and promotes browning of white adipose tissue in mice. Diabetologia 2014, 58, 149-157. [CrossRef] [PubMed]
61. Pasche, B., Wang, M., Pennison, M., Jimenez, H. Prevention and treatment of cancer with aspirin: Where do we stand? Semin. Oncol. 2014, 41, 397-401. [CrossRef] [PubMed]
62. Bosetti, C., Rosato, V., Gallus, S., Cuzick, J., La Vecchia, C. Aspirin and cancer risk: A quantitative review to 2011. Ann. Oncol. 2012, 23, 1403-1415. [CrossRef] [PubMed]
63. Rothwell, P.M., Fowkes, F.G.R., Belch, J.F.F., Ogawa, H., Warlow, C.P., Meade, T.W. Effect of daily aspirin on long-term risk of death due to cancer: Analysis of individual patient data from randomised trials. Lancet 2011, 377, 31-41. [CrossRef]
64. Gupta, R.A., Dubois, R.N. Colorectal cancer prevention and treatment by inhibition of cyclooxygenase-2. Nat. Rev. Cancer 2001, 1, 11-21. [CrossRef] [PubMed]
65. González-Correa, J.A., Navas, M.D., Muñoz-Marín, J., Trujillo, M., Fernández-Bolaños, J., de la Cruz, J.P. Effects of hydroxytyrosol and hydroxytyrosol acetate administration to rats on platelet function compared to acetylsalicylic acid. J. Agric. Food Chem. 2008, 56, 7872-7876. [CrossRef] [PubMed]
66. Tian, X.Y., Wong, W.T., Leung, F.P., Zhang, Y., Wang, Y.-X., Lee, H.K., Ng, C.F., Chen, Z.Y., Yao, X., Au, C.L., et al. Oxidative stress-dependent cyclooxygenase-2-derived prostaglandin f(2α) impairs endothelial function in renovascular hypertensive rats. Antioxid. Redox Signal. 2012, 16, 363-373. [CrossRef] [PubMed]
67. Martín-Peláez, S., Castañer, O., Konstantinidou, V., Subirana, I., Muñoz-Aguayo, D., Blanchart, G., Gaixas, S., de la Torre, R., Farré, M., Sáez, G.T., et al. Effect of olive oil phenolic compounds on the expression of blood pressure-related genes in healthy individuals. Eur. J. Nutr. 2015. [CrossRef] [PubMed]
68. Khymenets, O., Fitó, M., Covas, M.-I., Farré, M., Pujadas, M.-A., Muñoz, D., Konstantinidou, V., de la Torre, R. Mononuclear cell transcriptome response after sustained virgin olive oil consumption in humans: An exploratory nutrigenomics study. OMICS 2009, 13, 7-19. [CrossRef] [PubMed]
69. Farràs, M., Valls, R.M., Fernández-Castillejo, S., Giralt, M., Solà, R., Subirana, I., Motilva, M.-J., Konstantinidou, V., Covas, M.-I., Fitó, M. Olive oil polyphenols enhance the expression of cholesterol efflux related genes in vivo in humans. A randomized controlled trial. J. Nutr. Biochem. 2013, 24, 1334-1339. [CrossRef] [PubMed]
70. Tall, A.R., Yvan-Charvet, L. Cholesterol, inflammation and innate immunity. Nat. Rev. Immunol. 2015, 15, 104-116. [CrossRef] [PubMed]
71. Agard, M., Asakrah, S., Morici, L.A. PGE(2) suppression of innate immunity during mucosal bacterial infection. Front. Cell. Infect. Microbiol. 2013, 3, 45. [CrossRef] [PubMed]
72. Lee, S.M.Y., Gai, W.W., Cheung, T.K.W., Peiris, J.S.M. Antiviral effect of a selective COX-2 inhibitor on H5N1 infection in vitro. Antivir. Res. 2011, 91, 330-334. [CrossRef] [PubMed]
73. Ito, Y., Correll, K., Zemans, R.L., Leslie, C.C., Murphy, R.C., Mason, R.J. Influenza induces IL-8 and GM-CSF secretion by human alveolar epithelial cells through HGF/c-Met and TGF-α/EGFR signaling. Am. J. Physiol. Lung Cell. Mol. Physiol. 2015, 308, L1178-L1188. [CrossRef] [PubMed]
74. Vitaglione, P., Savarese, M., Paduano, A., Scalfi, L., Fogliano, V., Sacchi, R. Healthy virgin olive oil: A matter of bitterness. Crit. Rev. Food Sci. Nutr. 2015, 55, 1808-1818. [CrossRef] [PubMed]
75. Kirkland, D., Edwards, J., Woehrle, T., Beilstein, P. Investigations into the genotoxic potential of olive extracts. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2015, 777, 17-28. [CrossRef] [PubMed]
76. Camargo, A., Ruano, J., Fernandez, J.M., Parnell, L.D., Jimenez, A., Santos-Gonzalez, M., Marin, C., Perez-Martinez, P., Uceda, M., Lopez-Miranda, J., et al. Gene expression changes in mononuclear cells in patients with metabolic syndrome after acute intake of phenol-rich virgin olive oil. BMC Genom. 2010, 11, 253. [CrossRef] [PubMed]
77. Ritchie, M.E., Phipson, B., Wu, D., Hu, Y., Law, C.W., Shi, W., Smyth, G.K. Limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 2015. [CrossRef] [PubMed]
78. Edgar, R., Domrachev, M., Lash, A.E. Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2002, 30, 207-210. [CrossRef] [PubMed]
79. Gautier, L., Cope, L., Bolstad, B.M., Irizarry, R.A. affy-Analysis of Affymetrix GeneChip data at the probe level. Bioinformatics 2004, 20, 307-315. [CrossRef] [PubMed]
80. R Development Core Team. R: A Language and Environment for Statistical Computing. Found. Stat. Comput. 2013. Available online: http://www.r-project.org/ (accessed on 12 July 2016).
81. Wakefield, J. Bayes factors for genome-wide association studies: Comparison with p-values. Genet. Epidemiol. 2009, 33, 79-86. [CrossRef] [PubMed]
82. Krämer, A., Green, J., Pollard, J., Tugendreich, S. Causal analysis approaches in Ingenuity Pathway Analysis. Bioinformatics 2014, 30, 523-530. [CrossRef] [PubMed]
83. Kozera, B., Rapacz, M. Reference genes in real-time PCR. J. Appl. Genet. 2013, 54, 391-406. [CrossRef] [PubMed]
84. Nicot, N., Hausman, J.-F., Hoffmann, L., Evers, D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J. Exp. Bot. 2005, 56, 2907-2914. [CrossRef] [PubMed]