Substitution of a Single Amino Acid Reverses the Regiospecificity of the Baeyer-Villiger Monooxygenase PntE in the Biosynthesis of the Antibiotic Pentalenolactone

Biochemistry

Volumn 55, Issue 48, 2016, Pages 6696-6704

  Chen, Ke ^a   Wu, Shiwen ^a   Zhu, Lu ^a   Zhang, Chengde ^a   Xiang, Wensheng ^d   Deng, Zixin ^a   Ikeda, Haruo ^c   Cane, David E ^b   Zhu, Dongqing ^a  

^a WUHAN UNIVERSITY   (China)

^b BROWN UNIVERSITY   (United States)

^c KITASATO UNIVERSITY   (Japan)

^d NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CARBON; ESTERS; MIXTURES;

BAEYER VILLIGER OXIDATION; BAEYER-VILLIGER MONOOXYGENASES; DELETION MUTANTS; DESIGN AND ANALYSIS; FLAVIN-DEPENDENT; METHYLENE CARBONS; ORTHOLOGOUS PROTEINS; STREPTOMYCES EXFOLIATUS;

AMINO ACIDS;

MUTANT PROTEIN; PENTALENOLACTONE; PNTE PROTEIN; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; ANTIINFECTIVE AGENT; ARENAEMYCIN E; ARGININE; BACTERIAL PROTEIN; LYSINE; MIXED FUNCTION OXIDASE; SESQUITERPENE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; BACTERIAL GENE; CATALYSIS; DELETION MUTANT; IN VITRO STUDY; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; STEREOSPECIFICITY; STREPTOMYCES AVERMITILIS; BIOSYNTHESIS; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETIC COMPLEMENTATION; GENETICS; METABOLISM; MISSENSE MUTATION; SEQUENCE HOMOLOGY; STEREOISOMERISM; STREPTOMYCES;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; ARGININE; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; GENETIC COMPLEMENTATION TEST; LYSINE; MIXED FUNCTION OXYGENASES; MODELS, CHEMICAL; MOLECULAR STRUCTURE; MUTATION, MISSENSE; SEQUENCE HOMOLOGY, AMINO ACID; SESQUITERPENES; STEREOISOMERISM; STREPTOMYCES;

EID: 85002778491     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.6b01040     Document Type: Article

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