The N-terminal loop of IRAK-4 death domain regulates ordered assembly of the Myddosome signalling scaffold

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Dossang, Anthony C G ^a,b   Motshwene, Precious G ^a,c   Yang, Yang ^a   Symmons, Martyn F ^a   Bryant, Clare E ^a   Borman, Satty ^b   George, Julie ^d   Weber, Alexander N R ^e   Gay, Nicholas J ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c UNIVERSITY OF PRETORIA   (South Africa)

^d GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^e UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 1 RECEPTOR ASSOCIATED KINASE; IRAK4 PROTEIN, HUMAN; MULTIPROTEIN COMPLEX; MYD88 PROTEIN, HUMAN; MYELOID DIFFERENTIATION FACTOR 88;

CHEMISTRY; HUMAN; METABOLISM; PROTEIN MOTIF; PROTEIN QUATERNARY STRUCTURE; SIGNAL TRANSDUCTION; STATIC ELECTRICITY;

AMINO ACID MOTIFS; HUMANS; INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASES; MULTIPROTEIN COMPLEXES; MYELOID DIFFERENTIATION FACTOR 88; PROTEIN STRUCTURE, QUATERNARY; SIGNAL TRANSDUCTION; STATIC ELECTRICITY;

EID: 84996523762     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep37267     Document Type: Article

References (39)

1. Gay, N. J., Symmons, M. F., Gangloff, M. & Bryant, C. E. Assembly and localization of Toll-like receptor signalling complexes. Nature Rev. Immunol. 14, 546-558 (2014).
2. Kang, J. Y. & Lee, J. O. Structural biology of the Toll-like receptor family. Annu. Rev. Biochem. 80, 917-941 (2011).
3. Gay, N. J., Gangloff, M. & Weber, A. N. Toll-like receptors as molecular switches. Nature Rev. Immunol. 6, 693-698 (2006).
4. Motshwene, P. G. et al. An oligomeric signaling platform formed by the Toll-like receptor signal transducers MyD88 and IRAK-4. J. Biol. Chem. 284, 25404-25411 (2009).
5. Lin, S. C., Lo, Y. C. & Wu, H. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 465, 885-890 (2010).
6. Ferrao, R. & Wu, H. Helical assembly in the death domain (DD) superfamily. Curr. Opin. Struct. Biol. 22, 241-247 (2012).
7. Weber, C. H. & Vincenz, C. The death domain superfamily: a tale of two interfaces? Trends Biochem. Sci. 26, 475-481 (2001).
8. Park, H. H. et al. Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex. Cell 128, 533-546 (2007).
9. von Bernuth, H. et al. Pyogenic bacterial infections in humans with MyD88 deficiency. Science 321, 691-696 (2008).
10. Picard, C. et al. Pyogenic bacterial infections in humans with IRAK-4 deficiency. Science 299, 2076-2079 (2003).
11. Hoarau, C. et al. TLR9 activation induces normal neutrophil responses in a child with IRAK-4 deficiency: involvement of the direct PI3K pathway. J. Immunol. 179, 4754-4765 (2007).
12. Avbelj, M. et al. Activation of lymphoma-associated MyD88 mutations via allostery-induced TIR-domain oligomerization. Blood 124, 3896-3904 (2014).
13. Ngo, V. N. et al. Oncogenically active MYD88 mutations in human lymphoma. Nature 470, 115-119 (2011).
14. Ferrao, R. et al. IRAK4 Dimerization and trans-Autophosphorylation Are Induced by Myddosome Assembly. Mol. Cell 55, 891-903 (2014).
15. Kawagoe, T. et al. Essential role of IRAK-4 protein and its kinase activity in Toll-like receptor-mediated immune responses but not in TCR signaling. J. Exp. Med. 204, 1013-1024 (2007).
16. Kim, T. W. et al. A critical role for IRAK4 kinase activity in Toll-like receptor-mediated innate immunity. J. Exp. Med. 204, 1025-1036 (2007).
17. Cheng, H. et al. Regulation of IRAK-4 kinase activity via autophosphorylation within its activation loop. Biochem. Biophys. Res. Commun. 352, 609-616 (2007).
18. Gallwitz, M., Enoksson, M., Thorpe, M. & Hellman, L. The extended cleavage specificity of human thrombin. PLoS One 7, e31756 (2012).
19. George, J. et al. Two human MYD88 variants, S34Y and R98C, interfere with MyD88-IRAK4-myddosome assembly. J. Biol. Chem. 286, 1341-1353 (2011).
20. Lasker, M. V., Gajjar, M. M. & Nair, S. K. Cutting edge: molecular structure of the IL-1R-associated kinase-4 death domain and its implications for TLR signaling. J. Immunol. 175, 4175-4179 (2005).
21. Andrew, C. D., Warwicker, J., Jones, G. R. & Doig, A. J. Effect of phosphorylation on alpha-helix stability as a function of position. Biochemistry. 41, 1897-1905 (2002).
22. Hu, Q. et al. Molecular determinants of caspase-9 activation by the Apaf-1 apoptosome. Proc. Natl. Acad. Sci. USA 111, 16254-16261 (2014).
23. Mandell, D. J. et al. Strengths of hydrogen bonds involving phosphorylated amino acid side chains. J. Am. Chem. Soc. 129, 820-827 (2007).
24. Kagan, J. C., Magupalli, V. G. & Wu, H. SMOCs: supramolecular organizing centres that control innate immunity. Nature Rev. Immunol. 14, 821-826 (2014).
25. Monie, T. P., Bryant, C. E. & Gay, N. J. Activating immunity: lessons from the TLRs and NLRs. Trends Biochem. Sci. 34, 553-561 (2009).
26. Wang, L. et al. The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Nature Struct. Mol. Biol. 17, 1324-1329 (2010).
27. Yamamoto, T. et al. Functional assessment of the mutational effects of human IRAK4 and MyD88 genes. Mol. Immunol. 58, 66-76 (2014).
28. Barford, D., Hu, S. H. & Johnson, L. N. Structural mechanism for glycogen phosphorylase control by phosphorylation and AMP. J. Mol. Biol. 218, 233-260 (1991).
29. Zhou, H. et al. IRAK-M mediates Toll-like receptor/IL-1R-induced NFkappaB activation and cytokine production. EMBO J 32, 583-596 (2013).
30. Towb, P., Sun, H. & Wasserman, S. A. Tube Is an IRAK-4 homolog in a Toll pathway adapted for development and immunity. J Innate Immun. 1, 309-321 (2009).
31. Moncrieffe, M. C., Grossmann, J. G. & Gay, N. J. Assembly of Oligomeric Death Domain Complexes during Toll Receptor Signaling. J. Biol. Chem. 283, 33447-33454 (2008).
32. Sun, H., Towb, P., Chiem, D. N., Foster, B. A. & Wasserman, S. A. Regulated assembly of the Toll signaling complex drives Drosophila dorsoventral patterning. EMBO J. 23, 100-110 (2004).
33. Xiao, T., Towb, P., Wasserman, S. A. & Sprang, S. R. Three-dimensional structure of a complex between the death domains of Pelle and Tube. Cell 99, 545-555 (1999).
34. Edwards, D. N., Towb, P. & Wasserman, S. A. An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. Development 124, 3855-3864 (1997).
35. Daigneault, J., Klemetsaune, L. & Wasserman, S. A. The IRAK homolog Pelle is the functional counterpart of IkappaB kinase in the Drosophila Toll pathway. PLoS One 8, e75150 (2013).
36. Li, S. Y., Strelow, A., Fontana, E. J. & Wesche, H. IRAK-4: A novel member of the IRAK family with the properties of an IRAKkinase. Proc. Natl. Acad. Sci. USA 99, 5567-5572 (2002).
37. Shevchenko, A., Wilm, M., Vorm, O. & Mann, M. Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68, 850-858 (1996).
38. Wilm, M. et al. Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379, 466-469 (1996).
39. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66, 486-501 (2010).