Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in < 25 min

Journal of Microbiological Methods

Volumn 132, Issue , 2017, Pages 27-33

  Ahmad, Farhan ^a   Stedtfeld, Robert D ^a   Waseem, Hassan ^a   Williams, Maggie R ^a   Cupples, Alison M ^a   Tiedje, James M ^b,c   Hashsham, Syed A ^a,b  

^a Michigan State University   (United States)

^b MICHIGAN STATE UNIVERSITY   (United States)

^c NONE   (United States)

Author keywords

Detection limit; Loop mediated isothermal amplification; Most probable number; Point of care diagnostics; Propidium monoazide

Indexed keywords

GENOMIC DNA; AZIDE; BACTERIAL DNA; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

ARTICLE; COLONY FORMING UNIT; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA TEMPLATE; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FLUORESCENCE IMAGING; GENE AMPLIFICATION; HEAT TREATMENT; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MICROFLUIDICS; MOST PROBABLE NUMBER LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; ANALOGS AND DERIVATIVES; BACTERIAL COUNT; BACTERIAL GENE; CHEMISTRY; FOOD CONTAMINATION; FOOD CONTROL; ISOLATION AND PURIFICATION; MICROBIAL VIABILITY; MICROBIOLOGY; MICROFLUIDIC ANALYSIS; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO;

AZIDES; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FOOD CONTAMINATION; FOOD MICROBIOLOGY; GENES, BACTERIAL; MICROBIAL VIABILITY; MICROFLUIDIC ANALYTICAL TECHNIQUES; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SIGNAL-TO-NOISE RATIO; WATER MICROBIOLOGY;

EID: 84995776576     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2016.11.010     Document Type: Article

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