Sensitive and Inexpensive Molecular Test for Falciparum Malaria: Detecting Plasmodium falciparum DNA Directly from Heat-Treated Blood by Loop-Mediated Isothermal Amplification

Clinical Chemistry

Volumn 52, Issue 2, 2006, Pages 303-306

  Poon, Leo L M ^a   Wong, Bonnie W Y ^a   Ma, Edmund H T ^a   Chan, Kwok H ^a   Chow, Larry M C ^b   Abeyewickreme, Wimal ^c   Tangpukdee, Noppadon ^d   Yuen, Kwok Y ^a   Guan, Yi ^a   Looareesuwan, Sornchai ^d   Peiris, J S Malik ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

^c UNIVERSITY OF KELANIYA   (Sri Lanka)

^d MAHIDOL UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

PROTOZOAL DNA; RNA 18S;

ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; COST CONTROL; DIAGNOSTIC PROCEDURE; DNA DETERMINATION; GENE AMPLIFICATION; HEAT TREATMENT; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MAJOR CLINICAL STUDY; MALARIA FALCIPARUM; NUCLEOTIDE SEQUENCE; PLASMODIUM FALCIPARUM; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; TURBIDITY;

PLASMODIUM FALCIPARUM;

EID: 31844450172     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2005.057901     Document Type: Article

References (14)

1. World Health Organization. World malaria report. Geneva: WHO, 2005.
2. Guerin PJ, Olliaro P, Nosten F, Druilhe P, Laxminarayan R, Binka F, et al. Malaria: current status of control, diagnosis, treatment, and a proposed agenda for research and development. Lancet Infect Dis 2002;2:564-73.
3. Greenwood BM, Bojang K, Whitty CJ, Targett GA. Malaria. Lancet 2005;365: 1487-98.
4. Moody A. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 2002;15:66-78.
5. Thomson S, Lohmann RC, Crawford L, Dubash R, Richardson H. External quality assessment in the examination of blood films for malarial parasites within Ontario, Canada. 2000. Arch Pathol Lab Med 2000;124:57-60.
6. Marx A, Pewsner D, Egger M, Nuesch R, Bucher HC, Genton B, et al. Meta-analysis: accuracy of rapid tests for malaria in travelers returning from endemic areas. Ann Intern Med 2005;142:836-46.
7. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000;28:E63.
8. Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 2002;16: 223-9.
9. Iwasaki M, Yonekawa T, Otsuka K, Suzuki W, Nagamine K, Hase T, et al. Validation of the loop-mediated isothermal amplification method for single nucleotide polymorphism genotyping with whole blood. Genome Lett 2003; 2:119-26.
10. Ndao M, Bandyayera E, Kokoskin E, Gyorkos TW, MacLean JD, Ward BJ. Comparison of blood smear, antigen detection, and nested-PCR methods for screening refugees from regions where malaria is endemic after a malaria outbreak in Quebec, Canada. J Clin Microbiol 2004;42:2694-700.
11. Orago AS, Wattimah DN, Aloka PL, Gitau CW, Orago MA, Onyango TG. An analysis of haematological parameters in patients and individual residents of a Plasmodium falciparum malaria holoendemic area of western Kenya. Microbios 2001;106(Suppl 2):117-32.
12. Al-Soud WA, Radstrom P. Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 2001;39:485-93.
13. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 2001;289: 150-4.
14. Mori Y, Kitao M, Tomita N, Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods 2004;59:145-57.