메뉴 건너뛰기




Volumn 12, Issue 2, 2010, Pages 152-161

Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq

Author keywords

[No Author keywords available]

Indexed keywords

CARNITINE; DETERGENT; GENOMIC DNA; HEPARIN; MUTANT PROTEIN; TAQ POLYMERASE; TREHALOSE;

EID: 77649113149     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2010.090070     Document Type: Article
Times cited : (120)

References (39)
  • 1
    • 0033065725 scopus 로고    scopus 로고
    • The use of polymerase chain reaction to detect septicemia in critically ill patients
    • Cursons RT, Jeyerajah E, Sleigh JW: The use of polymerase chain reaction to detect septicemia in critically ill patients. Crit Care Med 1999, 27:937-940
    • (1999) Crit Care Med , vol.27 , pp. 937-940
    • Cursons, R.T.1    Jeyerajah, E.2    Sleigh, J.W.3
  • 3
  • 4
    • 0031608937 scopus 로고    scopus 로고
    • An introduction to PCR primer design and optimization of amplification reactions
    • Robertson JM, Walsh-Weller J: An introduction to PCR primer design and optimization of amplification reactions. Methods Mol Biol 1998, 98:121-154
    • (1998) Methods Mol Biol , vol.98 , pp. 121-154
    • Robertson, J.M.1    Walsh-Weller, J.2
  • 5
    • 34147134597 scopus 로고    scopus 로고
    • Prenatal diagnosis of common aneuploidies in transcervical samples using quantitative fluorescent-PCR analysis
    • Bussani C, Cioni R, Mattei A, Fambrini M, Marchionni M, Scarselli G: Prenatal diagnosis of common aneuploidies in transcervical samples using quantitative fluorescent-PCR analysis. Mol Diagn Ther 2007, 11:117-121
    • (2007) Mol Diagn Ther , vol.11 , pp. 117-121
    • Bussani, C.1    Cioni, R.2    Mattei, A.3    Fambrini, M.4    Marchionni, M.5    Scarselli, G.6
  • 6
    • 33646075447 scopus 로고    scopus 로고
    • Noninvasive genotyping of 9 Y-chromosome specific STR loci using circulatory fetal DNA in maternal plasma by multiplex PCR
    • Deng Z, Wu G, Li Q, Zhang X, Liang Y, Li D, Gao S, Lan Y: Noninvasive genotyping of 9 Y-chromosome specific STR loci using circulatory fetal DNA in maternal plasma by multiplex PCR. Prenat Diagn 2006, 26:362-368
    • (2006) Prenat Diagn , vol.26 , pp. 362-368
    • Deng, Z.1    Wu, G.2    Li, Q.3    Zhang, X.4    Liang, Y.5    Li, D.6    Gao, S.7    Lan, Y.8
  • 7
    • 0029858729 scopus 로고    scopus 로고
    • Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit. GeneReleaser, and the phenol-chloroform method
    • Kramvis A, Bukofzer S, Kew MC: Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit. GeneReleaser, and the phenol-chloroform method J Clin Microbiol 1996, 34:2731-2733
    • (1996) J Clin Microbiol , vol.34 , pp. 2731-2733
    • Kramvis, A.1    Bukofzer, S.2    Kew, M.C.3
  • 8
    • 0034747327 scopus 로고    scopus 로고
    • TaqMan 5′-nuclease human immunodeficiency virus type 1 PCR assay with phage-packaged competitive internal control for high-throughput blood donor screening
    • Drosten C, Seifried E, Roth WK: TaqMan 5′-nuclease human immunodeficiency virus type 1 PCR assay with phage-packaged competitive internal control for high-throughput blood donor screening. J Clin Microbiol 2001, 39:4302-4308
    • (2001) J Clin Microbiol , vol.39 , pp. 4302-4308
    • Drosten, C.1    Seifried, E.2    Roth, W.K.3
  • 10
    • 0028674028 scopus 로고
    • Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification
    • Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K: Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci 1994, 39:362-372
    • (1994) J Forensic Sci , vol.39 , pp. 362-372
    • Akane, A.1    Matsubara, K.2    Nakamura, H.3    Takahashi, S.4    Kimura, K.5
  • 11
    • 0031874238 scopus 로고    scopus 로고
    • Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis
    • Morata P, Queipo-Ortuno MI, De Dios CJ: Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol 1998, 36:2443-2446
    • (1998) J Clin Microbiol , vol.36 , pp. 2443-2446
    • Morata, P.1    Queipo-Ortuno, M.I.2    De Dios, C.J.3
  • 12
    • 0343938892 scopus 로고    scopus 로고
    • Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR
    • Al-Soud WA, Jonsson LJ, Radstrom P: Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol 2000, 38:345-350
    • (2000) J Clin Microbiol , vol.38 , pp. 345-350
    • Al-Soud, W.A.1    Jonsson, L.J.2    Radstrom, P.3
  • 14
    • 0035126283 scopus 로고    scopus 로고
    • Purification and characterization of PCR-inhibitory components in blood cells
    • Al-Soud WA, Radstrom P: Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 2001, 39:485-493
    • (2001) J Clin Microbiol , vol.39 , pp. 485-493
    • Al-Soud, W.A.1    Radstrom, P.2
  • 15
    • 10644231765 scopus 로고    scopus 로고
    • Early prediction of hepatitis C virus (HCV) infection relapse in nonresponders to primary interferon therapy by means of HCV RNA whole-blood analysis
    • Watkins-Riedel T, Ferenci P, Steindl-Munda P, Gschwantler M, Mueller C, Woegerbauer M: Early prediction of hepatitis C virus (HCV) infection relapse in nonresponders to primary interferon therapy by means of HCV RNA whole-blood analysis. Clin Infect Dis 2004, 39:1754-1760
    • (2004) Clin Infect Dis , vol.39 , pp. 1754-1760
    • Watkins-Riedel, T.1    Ferenci, P.2    Steindl-Munda, P.3    Gschwantler, M.4    Mueller, C.5    Woegerbauer, M.6
  • 17
    • 0030915955 scopus 로고    scopus 로고
    • Comparison of methods for extraction of nucleic acid from hemolytic serum for PCR amplification of hepatitis B virus DNA sequences
    • Klein A, Barsuk R, Dagan S, Nusbaum O, Shouval D, Galun E: Comparison of methods for extraction of nucleic acid from hemolytic serum for PCR amplification of hepatitis B virus DNA sequences. J Clin Microbiol 1997, 35:1897-1899
    • (1997) J Clin Microbiol , vol.35 , pp. 1897-1899
    • Klein, A.1    Barsuk, R.2    Dagan, S.3    Nusbaum, O.4    Shouval, D.5    Galun, E.6
  • 18
    • 0030689681 scopus 로고    scopus 로고
    • Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences
    • Cattaneo C, Craig OE, James NT, Sokol RJ: Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences. J Forensic Sci 1997, 42:1126-1135
    • (1997) J Forensic Sci , vol.42 , pp. 1126-1135
    • Cattaneo, C.1    Craig, O.E.2    James, N.T.3    Sokol, R.J.4
  • 19
    • 0034459011 scopus 로고    scopus 로고
    • Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat
    • Al-Soud WA, Rådström P: Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 2000, 38:4463-4470
    • (2000) J Clin Microbiol , vol.38 , pp. 4463-4470
    • Al-Soud, W.A.1    Rådström, P.2
  • 20
    • 0035369054 scopus 로고    scopus 로고
    • The enhancement of PCR amplification by low molecular weight amides
    • Chakrabarti R, Schutt CE: The enhancement of PCR amplification by low molecular weight amides. Nucleic Acids Res 2001, 29:2377-2381
    • (2001) Nucleic Acids Res , vol.29 , pp. 2377-2381
    • Chakrabarti, R.1    Schutt, C.E.2
  • 21
    • 0036216184 scopus 로고    scopus 로고
    • Chakrabarti R, Schutt CE: Novel sulfoxides facilitate GC-rich template amplification. Biotechniques 2002, 32:866, 868, 870-872, 874
    • Chakrabarti R, Schutt CE: Novel sulfoxides facilitate GC-rich template amplification. Biotechniques 2002, 32:866, 868, 870-872, 874
  • 23
    • 23844441591 scopus 로고    scopus 로고
    • The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: Application to in vitro combinatorial selection of aptamers
    • Kang J, Lee MS, Gorenstein DG: The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: application to in vitro combinatorial selection of aptamers. J Biochem Biophys Methods 2005, 64:147-151
    • (2005) J Biochem Biophys Methods , vol.64 , pp. 147-151
    • Kang, J.1    Lee, M.S.2    Gorenstein, D.G.3
  • 24
    • 33751165548 scopus 로고    scopus 로고
    • Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences
    • Musso M, Bocciardi R, Parodi S, Ravazzolo R, Ceccherini I: Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences. J Mol Diagn 2006, 8:544-550
    • (2006) J Mol Diagn , vol.8 , pp. 544-550
    • Musso, M.1    Bocciardi, R.2    Parodi, S.3    Ravazzolo, R.4    Ceccherini, I.5
  • 27
    • 63249099357 scopus 로고    scopus 로고
    • Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples
    • Kermekchiev MB, Kirilova LI, Vail EE, Barnes WM: Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples. Nucleic Acids Res 2009, 37:e40
    • (2009) Nucleic Acids Res , vol.37
    • Kermekchiev, M.B.1    Kirilova, L.I.2    Vail, E.E.3    Barnes, W.M.4
  • 29
    • 0025259663 scopus 로고
    • Improvement of PCR amplified DNA sequencing with the aid of detergents
    • Bachmann B, Luke W, Hunsmann G: Improvement of PCR amplified DNA sequencing with the aid of detergents. Nucleic Acids Res 1990, 18:1309
    • (1990) Nucleic Acids Res , vol.18 , pp. 1309
    • Bachmann, B.1    Luke, W.2    Hunsmann, G.3
  • 31
    • 0028326569 scopus 로고
    • Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in a minimal medium
    • Beumer RR, Te Giffel MC, Cox LJ, Rombouts FM, Abee T: Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in a minimal medium. Appl Environ Microbiol 1994, 60:1359-1363
    • (1994) Appl Environ Microbiol , vol.60 , pp. 1359-1363
    • Beumer, R.R.1    Te Giffel, M.C.2    Cox, L.J.3    Rombouts, F.M.4    Abee, T.5
  • 32
    • 0026620530 scopus 로고
    • DNA sequencing with Thermus acquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. 1988
    • Innis MS, Myambo KB, Gelfand DH, Brown MA: DNA sequencing with Thermus acquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. 1988. Biotechnology 1992, 24:6-10
    • (1992) Biotechnology , vol.24 , pp. 6-10
    • Innis, M.S.1    Myambo, K.B.2    Gelfand, D.H.3    Brown, M.A.4
  • 33
    • 3042614052 scopus 로고    scopus 로고
    • Trehalose is a potent PCR enhancer: Lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose
    • Spiess AN, Mueller N, Ivell R: Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose. Clin Chem 2004, 50:1256-1259
    • (2004) Clin Chem , vol.50 , pp. 1256-1259
    • Spiess, A.N.1    Mueller, N.2    Ivell, R.3
  • 34
    • 0028156957 scopus 로고
    • Amplification of DNA from whole blood
    • Burckhardt J: Amplification of DNA from whole blood. PCR Methods Appl 1994, 3:239-243
    • (1994) PCR Methods Appl , vol.3 , pp. 239-243
    • Burckhardt, J.1
  • 35
    • 0025705469 scopus 로고
    • Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites
    • Chen JZ, Herzenberg LA, Herzenberg LA: Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites. Nucleic Acids Res 1990, 18:3255-3260
    • (1990) Nucleic Acids Res , vol.18 , pp. 3255-3260
    • Chen, J.Z.1    Herzenberg, L.A.2    Herzenberg, L.A.3
  • 36
    • 0032517920 scopus 로고    scopus 로고
    • Recently, betaine has been introduced as an additive in different PCR strategies
    • Henke W, Loening SA: Recently, betaine has been introduced as an additive in different PCR strategies. Nucleic Acids Res 1998, 26:687
    • (1998) Nucleic Acids Res , vol.26 , pp. 687
    • Henke, W.1    Loening, S.A.2
  • 37
    • 0035836707 scopus 로고    scopus 로고
    • Directed evolution of polymerase function by compartmentalized self-replication
    • Ghadessy FJ, Ong JL, Holliger P: Directed evolution of polymerase function by compartmentalized self-replication. Proc Natl Acad Sci USA 2001, 98:4552-4557
    • (2001) Proc Natl Acad Sci USA , vol.98 , pp. 4552-4557
    • Ghadessy, F.J.1    Ong, J.L.2    Holliger, P.3
  • 38
    • 0033990382 scopus 로고    scopus 로고
    • Effects of ethidium bromide and SYBR Green I on different polymerase chain reaction systems
    • Nath K, Sarosy JW, Hahn J, Di Como CJ: Effects of ethidium bromide and SYBR Green I on different polymerase chain reaction systems. J Biochem Biophys Methods 2000, 42:15-29
    • (2000) J Biochem Biophys Methods , vol.42 , pp. 15-29
    • Nath, K.1    Sarosy, J.W.2    Hahn, J.3    Di Como, C.J.4
  • 39
    • 0031568919 scopus 로고    scopus 로고
    • Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
    • Ririe KM, Rasmussen RP, Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997, 245:154-160
    • (1997) Anal Biochem , vol.245 , pp. 154-160
    • Ririe, K.M.1    Rasmussen, R.P.2    Wittwer, C.T.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.