A new set of rDNA-NTS-based multiple integrative cassettes for the development of antibiotic-marker-free recombinant yeasts

Journal of Biotechnology

Volumn 233, Issue , 2016, Pages 190-199

  Moon, Hye Yun ^a   Lee, Dong Wook ^a   Sim, Gyu Hun ^a   Kim, Hong Jin ^a   Hwang, Jee Youn ^b   Kwon, Mun Gyeong ^b   Kang, Bo Kyu ^c   Kim, Jong Man ^c   Kang, Hyun Ah ^a  

^a Chung Ang University   (South Korea)

^b National Fisheries Research and Development Institute (NFRDI)   (South Korea)

^c Green Cross Veterinary Products Co Ltd   (South Korea)

Author keywords

Defective auxotrophic markers; Multiple integration; Recombinant yeast; Ribosomal DNA non transcribed spacer

Indexed keywords

ANTIBIOTICS; BIOMARKERS; GENE EXPRESSION; GENES; INTEGRATION; PROTEINS; RECOMBINANT PROTEINS; TRANSCRIPTION; VACCINES; VIRUSES;

ANTIBIOTIC RESISTANCE GENES; AUXOTROPHIC MARKERS; MULTIPLE INTEGRATIONS; ORAL ADMINISTRATION; RECOMBINANT YEAST; RIBOSOMAL DNA (RDNA); TRANSCRIBED SPACER; YEAST SACCHAROMYCES CEREVISIAE;

YEAST;

CAPSID PROTEIN; DNA; FUNGAL PROTEIN; HIGH MOBILITY GROUP B1 PROTEIN; HIS3 PROTEIN; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LEU2 PROTEIN; NON TRANSCRIBED SPACER; RED SPOTTED GROUPER NECROSIS VIRUS CAPSID PROTEIN; RIBOSOME DNA; TRP1 PROTEIN; UNCLASSIFIED DRUG; URA3 PROTEIN; RECOMBINANT PROTEIN; RIBOSOMAL SPACER DNA;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; FEMALE; GENE CASSETTE; GENE EXPRESSION; IMMUNE RESPONSE; MOUSE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; VIRUS NEUTRALIZATION; YEAST CELL; ANIMAL; BAGG ALBINO MOUSE; GENETIC ENGINEERING; GENETICS; METABOLISM; PROCEDURES;

ANIMALS; DNA, RIBOSOMAL SPACER; FEMALE; GENETIC ENGINEERING; MICE; MICE, INBRED BALB C; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84989940494     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.07.006     Document Type: Article

References (37)

1. Abrams, K., Harvell, J.D., Shriner, D., Wertz, P., Maibach, H., Maibach, H.I., Rehfeld, S.J., Effect of organic solvents on in vitro human skin water barrier function. J. Invest. Dermatol. 101 (1993), 609–613.
2. Aw, R., Polizzi, K.M., Can too many copies spoil the broth?. Microb. Cell Fact., 12, 2013, 128.
3. Bilusic, M., Heery, C.R., Arlen, P.M., Rauckhorst, M., Apelian, D., Tsang, K.Y., Tucker, J.A., Jochems, C., Schlom, J., Gulley, J.L., Madan, R.A., Phase I trial of a recombinant yeast-CEA vaccine (GI-6207) in adults with metastatic CEA-expressing carcinoma. Cancer Immunol. Immunother. 63 (2014), 225–234.
4. Blanquet, S., Antonelli, R., Laforet, L., Denis, S., Marol-Bonnin, S., Recombinant Saccharomyces cerevisiae secreting proteins or peptides as a new drug delivery system in the gut. J. Biotechnol. 110 (2004), 37–49.
5. Bleykasten-Grosshans, C., Friedrich, A., Schacherer, J., Genome-wide analysis of intraspecific transposon diversity in yeast. BMC. BMC Genomics, 14, 2013, 399.
6. Entian, K.D., Kötter, P., Yeast genetic strain and plasmid collections. Method Microbiol. 36 (2007), 629–666.
7. Gaggar, A., Coeshott, C., Apelian, D., Rodell, T., Armstrong, B.R., Shen, G., Subramanian, G.M., McHutchison, J.G., Safety, tolerability and immunogenicity of GS-4774, a hepatitis B virus-specific therapeutic vaccine, in healthy subjects: a randomized study. Vaccine 32 (2014), 4925–4931.
8. Galanie, S., Thodey, K., Trenchard, I.J., Filsinger Interrante, M., Smolke, C.D., Complete biosynthesis of opioids in yeast. Science 349 (2015), 1095–1100.
9. Hill, J., Donald, K.A., Griffiths, D.E., DMSO-enhanced whole cell yeast transformation. Nucleic Acids. Res., 19, 1991, 5791.
10. Kang, H.A., Choi, E.S., Hong, W.K., Kim, J.Y., Ko, S.M., Sohn, J.H., Rhee, S.K., Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 53 (2000), 575–582.
11. Kiflmariam, M.G., Yang, H., Zhang, Z., Gene delivery to dendritic cells by orally administered recombinant Saccharomyces cerevisiae in mice. Vaccine 31 (2013), 1360–1363.
12. Kim, S.K., Karadeniz, F., Biological importance and applications of squalene and squalane. Adv. Food Nutr. Res. 65 (2012), 223–233.
13. Kim, H., Yoo, S.J., Kang, H.A., Yeast synthetic biology for the production of recombinant therapeutic proteins. FEMS Yeast Res. 15 (2014), 1–16.
14. Kim, H.J., Lee, J.Y., Kang, H.A., Lee, Y., Park, E.J., Kim, H.J., Oral immunization with whole yeast producing viral capsid antigen provokes a stronger humoral immune response than purified viral capsid antigen. Lett. Appl. Microbiol. 58 (2014), 285–291.
15. Kudla, B., Nicolas, A., A multisite integrative cassette for the yeast Saccharomyces cerevisiae. Gene 119 (1992), 49–56.
16. Lopes, T.S., Klootwijk, J., Veenstra, A.E., van der Aar, P.C., van Heerikhuizen, H., Raue, H.A., Planta, R.J., High-copy number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression. Gene 79 (1989), 199–206.
17. Lopes, T.S., de Wijs, I.J., Steenhauer, S.I., Verbakel, J., Planta, R.J., Factors affecting the mitotic stability of high-copy number integration into the ribosomal DNA of Saccharomyces erevisiae. Yeast 12 (1996), 467–477.
18. Nicaud, J.M., Madzak, C., van den Broek, P., Gysler, C., Duboc, P., Niederberger, P., Gaillardin, C., Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2 (2002), 371–379.
19. Oliveira, P.H., Mairhofer, J., Marker-free plasmids for biotechnological applications-implications and perspectives. Trends Biotechnol. 31 (2013), 539–547.
20. Oliveira, C., Teixeira, J.A., Lima, N., Da Silva, N.A., Domingues, L., Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production. J. Biosci. Bioeng. 103 (2007), 318–324.
21. Patterson, R., Eley, T., Browne, C., Martineau, H.M., Werling, D., Oral application of freeze-dried yeast particles expressing the PCV2b Cap protein on their surface induce protection to subsequent PCV2b challenge in vivo. Vaccine 33 (2015), 6199–6205.
22. Petes, T.D., Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell 19 (1980), 765–774.
23. Polakowski, T., Stahl, U., Lang, C., Overexpressionof a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast. Appl. Microbiol. Biotechnol. 49 (1998), 66–71.
24. Ro, D.K., Paradise, E.M., Ouellet, M., Fisher, K.J., Newman, K.L., Ndungu, J.M., Ho, K.A., Eachus, R.A., Ham, T.S., Kirby, J., Chang, M.C., Withers, S.T., Shiba, Y., Sarpong, R., Keasling, J.D., Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 440 (2006), 940–943.
25. Schreuder, M.P., Deen, C., Boersma, W.J., Pouwels, P.H., Klis, F.M., Yeast expressing hepatitis B virus surface antigen determinants on its surface:implications for a possible oral vaccine. Vaccine 14 (1996), 383–388.
26. Shi, S., Liang, Y., Zhang, M.M., Ang, E.L., Zhao, H., A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae. Metab. Eng. 33 (2016), 19–27.
27. Shin, M.K., Yoo, H.S., Animal vaccines based on orally presented yeast recombinants. Vaccine 31 (2013), 4287–4292.
28. Siddiqui, M.S., Thodey, K., Trenchard, I., Smolke, C.D., Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 12 (2012), 144–170.
29. Stubbs, A.C., Martin, K.S., Coeshott, C., Skaates, S.V., Kuritzkes, D.R., Whole recombinant yeast vaccine activates dendritic cells and elicits protective cell-mediated immunity. Nature 7 (2001), 625–629.
30. Szczebara, F.M., Chandelier, C., Villeret, C., Masurel, A., Bourot, S., Duport, C., Blanchard, S., Groisillier, A., Testet, E., Costaglioli, P., Cauet, G., Degryse, E., Balbuena, D., Winter, J., Achstetter, T., Spagnoli, R., Pompon, D., Dumas, B., Total biosynthesis of hydrocortisone from a simple carbon source in yeast. Nat. Biotechnol. 21 (2003), 143–149.
31. Trckova, M., Faldyna, M., Alexa, P., Sramkova Zajacova, Z., Gopfert, E., Kumprechtova, D., Auclair, E., D'Inca, R., The effects of live yeast Saccharomyces cerevisiae on postweaning diarrhea, immune response, and growth performance in weaned piglets. J. Anim. Sci. 92 (2014), 767–774.
32. Tsai, C.S., Kong, I.I., Lesmana, A., Million, G., Zhang, G.C., Kim, S.R., Jin, Y.S., Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR. Biotechnol. Bioeng. 112 (2015), 2406–2411.
33. Venema, J., Tollervey, D., Processing of pre-ribosomal RNA in Saccharomyces cerevisiae. Yeast 11 (1995), 1629–1650.
34. Yan, N., Xu, K., Li, X., Liu, Y., Bai, Y., Zhang, X., Han, B., Chen, Z., Zhang, Z., Recombinant Saccharomyces cerevisiae serves as novel carrier for oral DNA vaccines in Carassius auratus. Fish Shellfish Immunol. 47 (2015), 758–765.
35. Yamada, R., Taniguchi, N., Tanaka, T., Ogino, C., Fukuda, H., Kondo, A., Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Microb. Cell Fact. 9 (2010), 32–39.
36. Zhang, T., Yang, H., Wang, R., Xu, K., Xin, Y., Ren, G., Oral administration of myostatin-specific whole recombinant yeast Saccharomyces cerevisiae vaccine increases body weight and muscle composition in mice. Vaccine 29 (2011), 8412–8416.
37. Zhang, T., Sun, L., Xin, Y., Ma, L., Zhang, Y., Wang, X., Xu, K., Ren, C., Zhang, C., Chen, Z., Yang, H., Zhang, Z., A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin. BMC Biotechnology 12 (2012), 97–103.