Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production

Journal of Bioscience and Bioengineering

Volumn 103, Issue 4, 2007, Pages 318-324

  Oliveira, Carla ^a   Teixeira, José A ^a   Lima, Nelson ^a   Da Silva, Nancy A ^b   Domingues, Lucília ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b University of California at Irvine   (United States)

Author keywords

Aspergillus niger galactosidase production; continuous high cell density culture; genetic stability of delta integrating systems; recombinant; Saccharomyces cerevisiae; yeast flocculation

Indexed keywords

ANTIBIOTICS; BIOREACTORS; CELL CULTURE;

ASPERGILLUS NIGER; CONTINUOUS HIGH-CELL-DENSITY CULTURE; PLASMID-CARRYING STRAIN; YEAST FLOCCULATION;

YEAST;

ANTIBIOTIC G 418; BETA GALACTOSIDASE;

ARTICLE; ASPERGILLUS NIGER; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; EXPRESSION VECTOR; FUNGAL GENE; FUNGUS CULTURE; GENOME ANALYSIS; LAC A GENE; NONHUMAN; PROTEIN EXPRESSION; RETROPOSON; SACCHAROMYCES CEREVISIAE; SOUTHERN BLOTTING;

ASPERGILLUS NIGER; BETA-GALACTOSIDASE; BIOREACTORS; FUNGAL PROTEINS; INDUSTRIAL MICROBIOLOGY; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS NIGER; SACCHAROMYCES CEREVISIAE;

EID: 34248213118     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.103.318     Document Type: Article

References (29)

1. Domingues L., Teixeira J.A., Penttilä M., and Lima N. Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase. Appl. Microbiol. Biotechnol. 58 (2002) 645-650
2. Domingues L., Oliveira C., Castro I., Lima N., and Teixeira J.A. Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose. J. Chem. Technol. Biotechnol. 79 (2004) 809-815
3. Domingues L., Lima N., and Teixeira J.A. Aspergillus niger β-galactosidase production in a continuous high yeast cell density reactor. Process Biochem. 40 (2005) 1151-1154
4. Domingues L., Lima N., and Teixeira J.A. Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain. Biotechnol. Bioeng. 64 (1999) 692-697
5. Vicente A.A., Mota M., and Teixeira J.A. Flocculation bioreactors. In: Cabral J.M.S., Mota M., and Tramper J. (Eds). Multiphase bioreactor design (2001), Taylor & Francis, London 363-389
6. Domingues L., Vicente A.A., Lima N., and Teixeira J.A. Applications of yeast flocculation in biotechnological processes. Biotechnol. Bioprocess Eng. 5 (2000) 288-305
7. Romanos M.A., Scorer C.A., and Clare J.J. Foreign gene expression in yeast: a review. Yeast 8 (1992) 423-488
8. Lopes T.S., Hakkaart G.J.A., Koerts B.L., Raué H., and Planta R.J. Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae. Gene 105 (1991) 83-90
9. Nieto A., Prieto J.A., and Sanz P. Stable high-copy-number integration of Aspergillus oryzae α-amylase cDNA in an industrial baker's yeast strain. Biotechnol. Prog. 15 (1999) 459-466
10. Sakai A., Schimizu Y., and Hishinuma F. Integration of heterologous genes into chromosome of Saccharomyces cerevisiae using delta sequence of yeast retrotransposon Ty. Appl. Microbiol. Biotechnol. 33 (1990) 302-306
11. Sakai A., Ozawa F., Higashizaki T., Shimizu Y., and Hishinuma F. Enhanced secretion of human nerve growth factor from Saccharomyces cerevisiae using delta-integration system. Bio/Technology 9 (1991) 1381-1385
12. Parekh R.N., Shaw M.R., and Wittrup K.D. An integrating vector for tunable, high copy, stable integration into dispersed Ty δ sites of Saccharomyces cerevisiae. Biotechnol. Prog. 12 (1996) 16-21
13. Wang X., Wang Z., and Da Silva N.A. G418 selection and stability of cloned genes integrated at chromosomal δ sequences of Saccharomyces cerevisiae. Biotechnol. Bioeng. 49 (1996) 45-51
14. Dujon B. The yeast genome project: what did we learn?. Trends Genet. 12 (1996) 263-270
15. Wyrick J.J., Aparicio J.G., Chen T., Barnett J.D., Jennings E.G., Young R.A., Bell S.P., and Aparicio O.M. Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping replication origins. Science 294 (2001) 2357-2360
16. Kumar V., Ramakrishnan S., Teeri T.T., Knowles J.K.C., and Hartley B.S. Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Bio/Technology 10 (1992) 82-85
17. Ausubel F., Brent R., Kingston R., Moore D., Seidman J.G., Smith J., and Struhl K. Current protocols in molecular biology. 4th ed (1997), John Wiley & Sons, New York
18. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31 (1959) 426-428
19. Hofman C.S., and Winston F. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57 (1987) 267-272
20. Kim Y.S., Kim S.Y., Kim J.H., and Kim S.C. Xylitol production using recombinant Saccharomyces cerevisiae containing multiple xylose reductase genes at chromosomal δ-sequences. J. Biotechnol. 67 (1999) 159-171
21. Cha K.-H., Kim M.-D., Lee T.-H., Lim H.-K., Jung K.-H., and Seo J.-H. Selection of optimum expression system for production of kringle fragment of human apolipoprotein(a) in Saccharomyces cerevisiae. Biotechnol. Bioprocess Eng. 9 (2004) 523-527
22. Cho K.M., Yoo Y.J., and Kang H.S. δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol. Enzyme Microb. Technol. 25 (1999) 23-30
23. Kimura T., Kitamoto N., Iimura Y., and Kito Y. Production of HM-1 killer toxin in Saccharomyces cerevisiae transformed with the PDR4 gene and δ-sequence-mediated multi-integration system. J. Ferment. Bioeng. 80 (1995) 423-428
24. Lee F.W.F., and Da Silva N.A. Improved efficiency and stability of multiple cloned gene insertions at the δ sequences of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 48 (1997) 339-345
25. Kim M.-D., Rhee S.-K., and Seo J.-H. Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal δ-integration. J. Biotechnol. 85 (2001) 41-48
26. Lee F.W.F., and Da Silva N.A. Sequential δ-Integration for the regulated insertion of cloned genes in Saccharomyces cerevisiae. Biotechnol. Prog. 13 (1997) 368-373
27. Chung Y.-S., Kim M.-D., Lee W.-J., Ryu Y.-W., Kim J.-H., and Seo J.-H. Stable expression of xylose reductase gene enhances xylitol production in recombinant Saccharomyces cerevisiae. Enzyme Microb. Technol. 30 (2002) 809-816
28. Saito S., Mieno Y., Nagashima T., Kumagai C., and Kitamoto K. Breeding of a new type of baker's yeast by δ-integration for overproduction of glucoamylase using a homotallic yeast. J. Ferment. Bioeng. 81 (1996) 98-103
29. Lú-Chau T.A., Guillán A., Núñez M.J., Roca E., and Lema J.M. Anaerobic and aerobic continuous cultures of Saccharomyces cerevisiae: comparison of plasmid stability and EXG1 gene expression. Bioprocess Biosyst. Eng. 26 (2004) 159-163