Factors affecting the mitotic stability of high-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae

Yeast

Volumn 12, Issue 5, 1996, Pages 467-477

  Lopes, T S ^a   De Wijs, I J ^a   Steenhauer, S I ^a   Verbakel, J ^b   Planta, R J ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

Author keywords

Heterologous protein; Plasmid: mitotic stability; Ribosomal DNA; Saccharomyces cerevisiae

Indexed keywords

RIBOSOME DNA;

ARTICLE; CONTROLLED STUDY; EXPRESSION VECTOR; MITOSIS RATE; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

DNA, FUNGAL; DNA, RIBOSOMAL; GENE DOSAGE; GENE EXPRESSION; GENE TARGETING; GENETIC VECTORS; MITOSIS; MOLECULAR WEIGHT; PLASMIDS; SACCHAROMYCES CEREVISIAE;

EID: 0029887178     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199604)12:5<467::AID-YEA933>3.0.CO;2-3     Document Type: Article

References (39)

1. Awane, K., Naito, A., Araki, H. and Oshima, Y. (1992). Automatic elimination of unnecessary bacterial sequences from yeast vectors. Gene 121, 161-165.
2. Broach, J. R. (1983). Construction of high copy vectors usina 2 μm circle sequences. Methods Enzymol. 101, 307-525.
3. Carter, B. L. A., Irani, M., Mackay, V. L., Seale, R. L., Sledziewski, A. V. and Smith, R. A. (1988). Expression and secretion of foreign genes in yeast. Agric. Biol. Chem. 50, 1503-1512.
4. Christman, M. F., Dietrich, F. S. and Fink, G. R. (1988). Mitotic recombination in the rDNA of S. cerevisiae is suppressed by a combined action of DNA topoisomerases I and II. Cell 55, 413-425.
5. DiBiasio, D. and Sardonni, C. (1986). Stability of continuous cultures with recombinant organisms. Biochem Eng. IV 469, 110-113.
6. Edens, L. and van der Wel, H. (1985). Microbial synthesis of the sweet-tasting plant protein thaumatin. TIBTECH 3, 1-4.
7. Edens, L., Brom, I., Ledeboer, A. M., et al. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell 40, 629-633.
8. Erhart, E. and Hollenberg, C. P. (1981). Curing of Saccharomyces cerevisiae 2 μm DNA by transformation. Current Genet. 3, 83-89.
9. Guarente, L., Yocum, R. and Gifford, P. A. (1982). GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulator as an upstream site. Proc. Natl. Acad. Sci. USA 79, 7410-7414.
10. Hanahan, D. (1982). Studies on transformation of E. coli with plasmids. J. Mol. Biol. 166, 557-580.
11. Johnston, M. (1987). A model fungal gene regulatory mechanism: The GAL genes of Saccharomyces cerevisiae. Microbiol Rev. 51, 458-467.
12. Keil, R. L. and Roeder, G. S. (1984). Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. cerevisiae. Cell 36, 377-386.
13. Kempers-Veenstra, A. E., van Heerikhuizen, H., Musters, W., Klootwijk, J. and Planta, R. J. (1984). Transcription of an artificial ribosomal RNA gene in yeast. EMBO J. 3, 1377-1382.
14. Kim, R. A. and Wang, J. C. (1989). A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal rings. Cell 57, 975-985.
15. Klebe, R. J., Harriss, J. V., Sharp, Z. D. and Douglas, M. G. A. (1983). General method for polyethyleneglycol-induced genetic transportation of bacteria and yeast. Gene 25, 333-341.
16. Kulkens, T., van Heerikhuizen, H., Klootwijk, J., Oliemans, J. and Planta, R. J. (1989). A yeast ribosomal DNA-binding protein that binds to the rDNA enhancer and also close to the site of the Pol I transcription initiation is not important for enhancer functioning. Curr. Genet. 16, 351-359.
17. Kulkens, T., van der Sande, C. A. F. M., Dekker, A. F., van Heerikhuizen, H. and Planta, R. J. (1992). A system to study transcription by yeast RNA polymerase 1 within the chromosomal context: functional analysis of the ribosomal DNA enhancer and the RBP1/REB1 binding sites. EMBO J. 11, 4665-4676.
18. Leer, R. (1985). Structural and putative regulatory sequences of yeast ribosomal protein genes. PhD Thesis, Vrije Universiteit, Amsterdam.
19. Liu, L. F. and Wang, J. C. (1987). Supercoiling of the DNA template during transcription. Proc. Natl. Acad. Sci. USA 84, 7024-7027.
20. Lopes, T. S. (1990). A novel ribosomal DNA vector for high expression of heterologous genes in yeast. PhD Thesis, Vrije Universiteit, Amsterdam.
21. Lopes, T. S., Klootwijk, J., Veenstra, A. E., et al. (1989). High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression. Gene 79, 199-206.
22. Lopes, T. S., Hakkaart, G.-J. A. J., Koerts, B. L., Raué, H. A. and Planta, R. J. (1991). Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae. Gene 105, 83-90.
23. Lusky, M. and Botchian, M. (1981). Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature 293, 79-81.
24. Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
25. Marres, C. A, M., van Loon, P. G. M., Oudshoorn, P., van Steeg, H., Grivell, L. A. and Slater, E. C. (1985). Nucleotide sequence analysis of the nuclear gene coding for manganese Superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein. Eur. J. Biochem. 147, 153-161.
26. Marsh, J., Ertle, M. and Weykes, E. (1984). The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32, 481-485.
27. Morrow, B. E., Johnson, S. P. and Warner, J. R. (1989) Proteins that bind to the yeast rDNA enhancer J. Biol. Chem. 264, 9061-9068.
28. Musters, W., Knol, J., Maas, P., Dekker, A. F., van Heerikhuizen, H. and Planta, R. J. (1989). Linker scanning of the yeast RNA polymerase I promoter, Nucl. Acids Res. 17, 410-414.
29. Orr-Weaver, T. C. and Szostak, J. W. (1983). Multiple, tandem plasmid integration in Saccharomyces cerevisiae. Mol. Cell. Biol. 3, 747-749.
30. Pedersen, M. B. (1983). DNA sequence polymorphisms in the genus Saccharomyces, I. Comparison of the HIS4 and ribosomal RNA genes in lager strains, ale strains and various species. Carlsberg Res. Commun 48, 485-503.
31. Ponticelli, A. S. and Smith, G. R. (1992). Chromosomal context dependence of a eukaryotic recombinational hot spot. Proc. Nail. Acad. Sci. USA 89, 227-231.
32. Scholte, B. J., te Velde, B., Groot, G. S. P. and de Vries. J. (1985). Induction of cytochrome P-450e-type isozymes by polychlorinated biphenyls in rat liver. Eur. J. Biochem. 151, 67-73.
33. Som, T., Armstrong, K. A., Volkert, F. C. and Broach, J. R. (1988). Auto-regulation of 2 μm circle gene expression provides a model for maintenance of stable plasmid copy levels. Cell 52, 27-37.
34. Stewart, S. E. and Roeder, G. S. (1989). Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae Mol. Cell. Biol 9, 3464-3472.
35. van der Sande, C. A. F. M., Kulkens, T., Kramer, A. B , et al. (1989). Termination of transcription by yeast RNA polymerase I. Nucl. Acids Res. 17, 9127-9146.
36. Vieira, J. and Messing, J. (1982). The pUC plasmids. an M13mp7-derived system for insertion, mutagenesis and sequencing with synthetic universal primers. Gene 19, 257-268.
37. Voelkel-Meiman, K., Keil, R. L. and Roeder, G. S. (1987). Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I. Cell 48, 1071-1079.
38. Voelkel-Meiman, K. and Roeder, G. S. (1990). Gene conversion tracts stimulated by Hotl-promoted transcription are long and continuous. Genetics 126, 851-867.
39. Walmsley, R. M., Gardner, D. C. J. and Oliver, S. G. (1983). Stability of a cloned gene in yeast grown in chemostat culture. Mol. Gen. Genet. 192, 361-365.