Development of a 3D tissue culture-based high-content screening platform that uses phenotypic profiling to discriminate selective inhibitors of receptor tyrosine kinases

Journal of Biomolecular Screening

Volumn 21, Issue 9, 2016, Pages 912-922

  Booij, Tijmen H ^a   Klop, Maarten J D ^b   Yan, Kuan ^b   Szántai Kis, Csaba ^c   Szokol, Balint ^c   Orfi, Laszlo ^c,d   Van De Water, Bob ^a   Keri, Gyorgy ^c,d   Price, Leo S ^a,b  

^a LEIDEN UNIVERSITY   (Netherlands)

^b OcellO BV   (Netherlands)

^c Vichem Chemie Research Ltd   (Hungary)

^d SEMMELWEIS UNIVERSITY   (Hungary)

Author keywords

3D tissue culture; c Met; EGFR; high content screening; phenotypic profiling

Indexed keywords

4 (3 CHLOROANILINO) 6,7 DIMETHOXYQUINAZOLINE; EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR KINASE INHIBITOR; ERLOTINIB; GEFITINIB; SCATTER FACTOR; SCATTER FACTOR RECEPTOR; EGFR PROTEIN, HUMAN; PROTEIN KINASE INHIBITOR;

ARTICLE; CANCER CELL; CANCER CELL CULTURE; DRUG SCREENING; ENZYME ACTIVITY; HUMAN; HYDROGEL; IMAGE ANALYSIS; IN VITRO STUDY; MORPHOLOGY; PHENOTYPE; PHENOTYPIC PROFILING; PRIORITY JOURNAL; PROSTATE ADENOCARCINOMA; TISSUE CULTURE; WESTERN BLOTTING; ANTAGONISTS AND INHIBITORS; CELL PROLIFERATION; CHEMISTRY; DRUG EFFECTS; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; MALE; MOLECULAR LIBRARY; PROCEDURES; PROSTATE TUMOR; THERAPEUTIC USE;

CELL PROLIFERATION; DRUG SCREENING ASSAYS, ANTITUMOR; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROGELS; MALE; PROSTATIC NEOPLASMS; PROTEIN KINASE INHIBITORS; PROTO-ONCOGENE PROTEINS C-MET; RECEPTOR, EPIDERMAL GROWTH FACTOR; SMALL MOLECULE LIBRARIES;

EID: 84988515925     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057116657269     Document Type: Article

References (30)

1. Mishra D. K., Sakamoto J. H., Thrall M. J., et al. Human Lung Cancer Cells Grown in an Ex Vivo 3D Lung Model Produce Matrix Metalloproteinases Not Produced in 2D Culture. PLoS One. 2012, 7, e45308.
2. Bradbury P., Fabry B., O'Neill G. M., Occupy Tissue: The Movement in Cancer Metastasis. Cell Adh. Migr. 2012, 6, 424-432.
3. Smalley K. S., Lioni M., Herlyn M., Life Isn't Flat: Taking Cancer Biology to the Next Dimension. In Vitro Cell Dev. Biol. Anim. 2006, 42, 242-247.
4. Breslin S., O'Driscoll L., Three-Dimensional Cell Culture: The Missing Link in Drug Discovery. Drug Discov. Today. 2013, 18, 240-249.
5. Li L., Zhou Q., Voss T. C., et al. High-Throughput Imaging: Focusing in on Drug Discovery in 3D. Methods 2016, 96, 97-102.
6. Taubenberger A. V., Bray L. J., Haller B., et al. 3D Extracellular Matrix Interactions Modulate Tumour Cell Growth, Invasion and Angiogenesis in Engineered Tumour Microenvironments. J. Act. Bio. 2016, 36, 73-85.
7. Debnath J., Brugge J. S., Modelling Glandular Epithelial Cancers in Three-Dimensional Cultures. Nat. Rev. 2005, 5, 675-688.
8. Schmeichel K. L., Bissell M. J., Modeling Tissue-Specific Signaling and Organ Function in Three Dimensions. J. Cell Sci. 2003, 116 (Pt 12), 2377-2388.
9. O'Brien L. E., Zegers M. M., Mostov K. E., Opinion: Building Epithelial Architecture: Insights from Three-Dimensional Culture Models. Nat. Rev. Mol. Cell Biol. 2002, 3, 531-537.
10. Härmä V., Knuuttila M., Virtanen J., et al. Lysophosphatidic Acid and Sphingosine-1-Phosphate Promote Morphogenesis and Block Invasion of Prostate Cancer Cells in Three-Dimensional Organotypic Models. Oncogene 2014, 31, 2075-2089.
11. Ren Y., Cao B., Law S., et al. Hepatocyte Growth Factor Promotes Cancer Cell Migration and Angiogenic Factors Expression: A Prognostic Marker of Human Esophageal Squamous Cell Carcinomas. Clin. Cancer Res. 2005, 11, 6190-6197.
12. Chan G. K. Y., Lutterbach B. A., Pan B.-S., et al. High-Throughput Analysis of HGF-Stimulated Cell Scattering. J. Biomol. Screen. 2008, 13, 847-854.
13. Radtke S., Milanovic M., Rossé C., et al. ERK2 but Not ERK1 Mediates HGF-Induced Motility in Non-Small Cell Lung Carcinoma Cell Lines. J. Cell Sci. 2013, 126, 2381-2391.
14. Zaritsky A., Natan S., Ben-Jacob E., et al. Emergence of HGF/SF Induced Coordinated Cellular Motility. PLoS One 2012, 7, e44671.
15. Comoglio P. M., Trusolino L., Invasive Growth: From Development to Metastasis. J. Clin. Invest. 2002, 109, 857-862.
16. Hardy K. M., Booth B. W., Hendrix M. J. C., et al. ErbB/EGF Signaling and EMT in Mammary Development and Breast Cancer. J. Mammary Gland. Biol. Neoplasia. 2010, 25, 191-199.
17. Appert-Collin A., Hubert P., Crémel G., et al. Role of ErbB Receptors in Cancer Cell Migration and Invasion. Front. Pharmacol. 2015, 6, 1-10.
18. Zi D., Klop M. J., Rogkoti M., et al. Ultra High Content Analysis and Phenotype Profiling of 3D Cultured Micro-Tissues. PLoS One 2014, 9, e109688.
19. Venables W. N., Ripley B. D., Modern Applied Statistics with S; Springer: New York, 2002; 4th ed.
20. Wickham H., ggplot2: Elegant Graphics for Data Analysis; Springer-Verlag: New York, 2009.
21. Ligges U., Maechler M., Scatterplot3d-An R Package for Visualizing Multivariate Data. J. Stat. Softw. 2003, 8 (11), 1-20.
22. Adjei A. A., Schwartz B., Garmey E., Early Clinical Development of ARQ 197, a Selective, Non-ATP-Competitive Inhibitor Targeting MET Tyrosine Kinase for the Treatment of Advanced Cancers. Oncologist 2011, 16, 788-799.
23. Tu W. H., Zhu C., Clark C., et al. Efficacy of c-Met Inhibitor for Advanced Prostate Cancer. BMC Cancer 2010, 10, 556.
24. Yang Y., Wislez M., Fujimoto N., et al. A Selective Small Molecule Inhibitor of c-Met, PHA-665752, Reverses Lung Premalignancy Induced by Mutant K-ras. Mol. Cancer Ther. 2008, 7, 952-960.
25. Caja L., Sancho P., Bertran E., et al. The Tyrphostin AG1478 Inhibits Proliferation and Induces Death of Liver Tumor Cells through EGF Receptor-Dependent and Independent Mechanisms. Biochem. Pharmacol. 2011, 82, 1583-1592.
26. Feldman D. R., Einhorn L. H., Qiunn D. I., et al. A Phase 2 Multicenter Study of Tivantinib (ARQ 197) Monotherapy in Patients with Relapsed or Refractory Germ Cell Tumors. Invest. New Drugs, 2013, 31, 1016-1022.
27. Sirenko O., Mitlo T., Hesley J., et al. High-Content Assays for Characterizing the Viability and Morphology of 3D Cancer Spheroid Cultures. Assay Drug Dev. Technol. 2015, 13, 402-414.
28. Härmä V., Schukov H.-P., Happonen A., et al. Quantification of Dynamic Morphological Drug Responses in 3D Organotypic Cell Cultures by Automated Image Analysis. PLoS One. 2014, 9, e96426.
29. Robinson S., Guyon L., Nevalainen J., et al. Segmentation of Image Data from Complex Organotypic 3D Models of Cancer Tissues with Markov Random Fields. PLoS One 2015, 10, e0143798.
30. Krausz E., de Hoogt R., Gustin E., et al. Translation of a Tumor Microenvironment Mimicking 3D Tumor Growth Co-Culture Assay Platform to High-Content Screening. J. Biomol. Screen. 2013, 18, 54-66.