Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration

Applied Microbiology and Biotechnology

Volumn 101, Issue 2, 2017, Pages 559-568

  Chen, Lin ^a   Zeng, An Ping ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Escherichia coli; L tryptophan; Metabolic analysis; Rational metabolic engineering

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; BIOSYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; METABOLISM; PRODUCTIVITY; SUBSTRATES; TIME SERIES ANALYSIS;

BIOSYNTHESIS PATHWAYS; BIOSYNTHETIC PRECURSORS; EXTRACELLULAR CONCENTRATION; FED-BATCH FERMENTATION; INTRACELLULAR CONCENTRATION; L-TRYPTOPHAN; METABOLIC ANALYSIS; MOLECULAR ENGINEERING;

METABOLIC ENGINEERING;

3 DEOXY 7 PHOSPHOHEPTULONATE SYNTHASE; ANTHRANILATE SYNTHASE; CHORISMIC ACID; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; GLUTAMINE; PHENYLALANINE; PHOSPHOENOLPYRUVATE; SERINE; SHIKIMIC ACID; TRYPTOPHAN; TYROSINE; ANTIDEPRESSANT AGENT; CULTURE MEDIUM;

AMINO ACID; BIOLOGICAL PRODUCTION; COLIFORM BACTERIUM; CONCENTRATION (COMPOSITION); EXPERIMENTAL DESIGN; FERMENTATION; GENETIC ENGINEERING; METABOLISM; MOLECULAR ANALYSIS;

ARTICLE; BIOMASS; CELL GROWTH; ESCHERICHIA COLI; FED BATCH FERMENTATION; GLUCOSE INTAKE; METABOLIC ENGINEERING; NONHUMAN; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; CYTOSOL; FERMENTATION; GENETICS; METABOLISM; PROCEDURES;

ESCHERICHIA COLI;

ANTIDEPRESSIVE AGENTS, SECOND-GENERATION; BIOSYNTHETIC PATHWAYS; CULTURE MEDIA; CYTOSOL; ESCHERICHIA COLI; FERMENTATION; GLUTAMIC ACID; METABOLIC ENGINEERING; TRYPTOPHAN;

EID: 84986294149     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-016-7772-5     Document Type: Article

References (56)

1. Aiba S, Imanaka T, Tsunekawa H (1980) Enhancement of tryptophan production by Escherichia coli as an application of genetic engineering. Biotechnol Lett 2:525–530
2. Al-Rabiee R, Lee EJ, Grant GA (1996) The mechanism of velocity modulated allosteric regulation in D-3-phosphoglycerate dehydrogenase. Cross-linking adjacent regulatory domains with engineered disulfides mimics effector binding. J Biol Chem 271:13013–13017
3. Azuma S, Tsunekawa H, Okabe M, Okamoto R, Aiba S (1993) Hyper-production of L-tryptophan via fermentation with crystallization. Appl Microbiol Biotechnol 39:471–476
4. Baker TI, Crawford IP (1966) Anthranilate synthetase. Partial purification and some kinetic studies on the enzyme from Escherichia coli. J Biol Chem 241:5577–5584
5. Bennett BD, Yuan J, Kimball EH, Rabinowitz JD (2008) Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc 3:1299–1311
6. Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD (2009) Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol 5:593–599
7. Berry A (1996) Improving production of aromatic compounds in Escherichia coli by metabolic engineering. Trends Biotechnol 14:250–256
8. Bertrand K, Yanofsky C (1976) Regulation of transcription termination in leader region of tryptophan operon of Escherichia coli involves tryptophan or its metabolic product. J Mol Biol 103:339–349
9. Bommareddy RR, Chen Z, Rappert S, Zeng A-P (2014) A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Metab Eng 25:30–37
10. Caligiuri MG, Bauerle R (1991a) Identification of amino acid residues involved in feedback regulation of the anthranilate synthase complex from Salmonella typhimurium. Evidence for an amino-terminal regulatory site*. J Biol Chem 266:8328–8335
11. Caligiuri MG, Bauerle R (1991b) Subunit communication in the anthranilate synthase complex from Salmonella typhimurium. Science 252:1845–1848
12. Chan EC, Tsai HL, Chen SL, Mou DG (1993) Amplification of the tryptophan operon gene in Escherichia coli chromosome to increase L-tryptophan biosynthesis. Appl Microbiol Biotechnol 40:301–305
13. Cheng L, Wang J, Xu Q, Xie X, Zhang Y, Zhao C, Chen N (2012) Effect of feeding strategy on l-tryptophan production by recombinant Escherichia coli. Ann Microbiol:1–10
14. Cheng L, Wang J, Xu Q, Zhao C, Shen Z, Xie X, Chen N (2013) Strategy for pH control and pH feedback-controlled substrate feeding for high-level production of L-tryptophan by Escherichia coli. World J Microbiol Biotechnol 29:883–890
15. da Luz JA, Hans E, Zeng A (2014) Automated fast filtration and on-filter quenching improve the intracellular metabolite analysis of microorganisms. Eng Life Sci 14:135–142
16. DeFeyter RC, Pittard J (1986) Purification and properties of shikimate kinase II from Escherichia coli K-12. J Bacteriol 165:331–333
17. Dodge TC, Gerstner JM (2002) Optimization of the glucose feed rate profile for the production of tryptophan from recombinant E coli. J Chem Technol Biotechnol 77:1238–1245
18. Fang M, Zhang C, Yang S, Cui J, Jiang P, Lou K, Wachi M, Xing X (2015) High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microb Cell Factories 14:8
19. Fang M, Wang T, Zhang C, Bai J, Zheng X, Zhao X, Lou C, Xing X (2016) Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli. Metab Eng 33:41–51
20. Ger YM, Chen SL, Chiang HJ, Shiuan D (1994) A single ser-180 mutation desensitizes feedback inhibition of the phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase in Escherichia coli. J Biochem 116:986–990
21. Grant GA, Schuller DJ, Banaszak LJ (1996) A model for the regulation of D-3-phosphoglycerate dehydrogenase, a Vmax-type allosteric enzyme. Protein Sci 5:34–41
22. Gu P, Yang F, Kang J, Wang Q, Qi Q (2012) One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microb Cell Factories 11:30
23. Gu P, Yang F, Li F, Liang Q, Qi Q (2013) Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production. Appl Microbiol Biotechnol 97:6677–6683
24. Hasegawa S, Suda M, Uematsu K, Natsuma Y, Hiraga K, Jojima T, Inui M, Yukawa H (2013) Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions. Appl Environ Microbiol 79:1250–1257
25. Heermann R, Zeppenfeld T, Jung K (2008) Simple generation of site-directed point mutations in the Escherichia coli chromosome using red®/ET® recombination. Microb Cell Factories 7:14
26. Ikeda M (2006) Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering. Appl Microbiol Biotechnol 69:615–626
27. Ikeda M, Katsumata R (1999) Hyperproduction of tryptophan by Corynebacterium glutamicum with the modified pentose phosphate pathway. Appl Environ Microbiol 65:2497–2502
28. Ikeda M, Nakanishi K, Kino K, Katsumata R (1994) Fermentative production of tryptophan by a stable recombinant strain of Corynebacterium glutamicum with a modified serine-biosynthetic pathway. Biosci Biotechnol Biochem 58:674–678
29. Kind S, Neubauer S, Becker J, Yamamoto M, Völkert M, Gv A, Zelder O, Wittmann C (2014) From zero to hero - production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum. Metab Eng 25:113–123
30. Kumamoto AA, Miller WG, Gunsalus RP (1987) Escherichia coli Tryptophan repressor binds multiple sites within the aroH and trp operators. Genes Dev 1:556–564
31. Kwak JH, Hong KW, Lee SH, Hong JH, Lee SY (1999) Identification of amino acid residues involved in feedback inhibition of the anthranilate synthase in Escherichia coli. J Biochem Mol Biol 32:20–24
32. Lawley B, Pittard AJ (1994) Regulation of aroL expression by TyrR protein and trp repressor in Escherichia coli K-12. J Bacteriol 176:6921–6930
33. Lee KH, Park JH, Kim TY, Kim HU, Lee SY (2007) Systems metabolic engineering of Escherichia coli for L-threonine production. Mol Syst Biol 3:149
34. Liu Q, Cheng Y, Xie X, Xu Q, Chen N (2012a) Modification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli. Bioresour Technol 114:549–554
35. Liu Q, Cheng Y, Xu Q, Xie X, Chen N (2012b) Effects of aroP gene disruption on L-tryptophan fermentation. Front Chem Sci Eng 6:158–162
36. Luo W, Huang J, Zhu X, Huang L, Cai J, Xu Z (2013) Enhanced production of l-tryptophan with glucose feeding and surfactant addition and related metabolic flux redistribution in the recombinant Escherichia coli. Food Sci Biotechnol 22:207–214
37. McCandliss RJ, Poling MD, Herrmann KM (1978) 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification and molecular characterization of the phenylalanine-sensitive isoenzyme from Escherichia coli. J Biol Chem 253:4259–4265
38. Nagaraja P, Yathirajan HS, Vasantha RA (2003) Highly sensitive reaction of tryptophan with p-phenylenediamine. Anal Biochem 312:157–161
39. O’Neil MJ (2006) The Merck index. An encyclopedia of chemicals, drugs, and biologicals, 14th edn. Merck, Whitehouse Station, p 1682
40. Park JH, Lee KH, Kim TY, Lee SY (2007) Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A 104:7797–7802
41. Park JH, Oh JE, Lee KH, Kim JY, Lee SY (2012) Rational Design of Escherichia coli for L -isoleucine production. ACS Synth Biol 1:532–540
42. Pearson JT, Dabrowski MJ, Kung I, Atkins WM (2005) The central loop of Escherichia coli glutamine synthetase is flexible and functionally passive. Arch Biochem Biophys 436:397–405
43. Platt T (1981) Termination of transcription and its regulation in the tryptophan operon of Escherichia coli. Cell 24:10–23
44. Porter RJ, Mulder RT, Joyce PR, Luty SE (2005) Tryptophan and tyrosine availability and response to antidepressant treatment in major depression. J Affect Disord 86:129–134
45. Ray JM, Yanofsky C, Bauerle R (1988) Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. J Bacteriol 170:5500–5506
46. Rodrigues AL, Trachtmann N, Becker J, Lohanatha AF, Blotenberg J, Bolten CJ, Korneli C, de Souza L, André O, Porto LM, Sprenger GA, Wittmann C (2013) Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein. Metab Eng 20:29–41
47. Rodriguez A, Martínez JA, Flores N, Escalante A, Gosset G, Bolivar F (2014) Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds. Microb Cell Factories 13:126
48. Schoner R, Herrmann KM (1976) 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli. J Biol Chem 251:5440–5447
49. Schuster S (1999) Detection of elementary flux modes in biochemical networks: a promising tool for pathway analysis and metabolic engineering. Trends Biotechnol 17:53–60
50. Shen T, Liu Q, Xie X, Xu Q, Chen N (2012) Improved production of tryptophan in genetically engineered Escherichia coli with TktA and PpsA overexpression. J Biomed Biotechnol 2012:605219
51. Wang J, Cheng L, Wang J, Liu Q, Shen T, Chen N (2013) Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Appl Microbiol Biotechnol 97:7587–7596
52. Yu D, Ellis HM, Lee E, Jenkins NA, Copeland NG, Court DL (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U S A 97:5978–5983
53. Zhang Y, Muyrers JPP, Rientjes J, Stewart AF (2003) Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells. BMC Mol Biol 4:1
54. Zhao ZJ, Zou C, Zhu YX, Dai J, Chen S, Wu D, Wu J, Chen J (2011) Development of L-tryptophan production strains by defined genetic modification in Escherichia coli. J Ind Microbiol Biotechnol 38:1921–1929
55. Zhao Z, Chen S, Wu D, Wu J, Chen J (2012) Effect of gene knockouts of L-tryptophan uptake system on the production of L-tryptophan in Escherichia coli. Process Biochem 47:340–344
56. Zurawski G, Elseviers D, Stauffer GV, Yanofsky C (1978) Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon. Proc Natl Acad Sci U S A 75:5988–5992