A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase

Metabolic Engineering

Volumn 25, Issue , 2014, Pages 30-37

  Bommareddy, Rajesh Reddy ^a   Chen, Zhen ^a   Rappert, Sugima ^a   Zeng, An Ping ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Cofactor availability; Glyceraldehyde 3 phosphate dehydrogenase; Lysine; Rational protein design

Indexed keywords

AMINO ACIDS; ISOTOPES; MOLECULAR BIOLOGY; PRODUCT DESIGN; PROTEINS;

CATALYTIC EFFICIENCIES; COFACTORS; CORYNEBACTERIUM GLUTAMICUM; GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE; LYSINE; METABOLIC FLUX ANALYSIS; PENTOSE PHOSPHATE PATHWAY; PROTEIN DESIGN;

AVAILABILITY;

CARBON 13; CARBON DIOXIDE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; ISOCITRATE DEHYDROGENASE; LYSINE; MALATE DEHYDROGENASE (DECARBOXYLATING); NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE (NADP);

ARTICLE; BATCH FERMENTATION; BINDING SITE; CARBON DIOXIDE EVOLUTION; CELL METABOLISM; CITRIC ACID CYCLE; COENZYME; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; GLUCOSE INTAKE; GLYCOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOMOLOGOUS RECOMBINATION; HYDROGEN BOND; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; MOLECULAR DOCKING; MOLECULAR MODEL; NONHUMAN; PENTOSE PHOSPHATE CYCLE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STEREOSPECIFICITY; BIOSYNTHESIS; GENETIC ENHANCEMENT; GENETICS; METABOLISM; PHYSIOLOGY; PROCEDURES; SIGNAL TRANSDUCTION;

CORYNEBACTERIUM GLUTAMICUM; GENETIC ENHANCEMENT; GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (NADP+); LYSINE; METABOLIC ENGINEERING; NADP; SIGNAL TRANSDUCTION;

EID: 84903743153     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2014.06.005     Document Type: Article

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