Cloning large gene clusters from E. coli using in vitro single-Strand overlapping annealing

ACS Synthetic Biology

Volumn 1, Issue 7, 2012, Pages 291-295

  Wang, Rui Yan ^a   Shi, Zhen Yu ^a   Chen, Jin Chun ^a   Chen, Guo Qiang ^a,b  

^a Tsinghua University   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Escherichia coli; Gene cluster; Homologous recombination; NADH:ubiquinone oxidoreductase; Single strand overlapping annealing; Synthetic biology

Indexed keywords

DNA FRAGMENT;

ARTICLE; COMPLEX I DEFICIENCY; COVALENT BOND; ESCHERICHIA COLI; GENE CLUSTER; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SINGLE STRAND OVERLAPPING ANNEALING;

BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENETIC ENGINEERING; GENOME, BACTERIAL; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; SYNTHETIC BIOLOGY;

ESCHERICHIA COLI;

EID: 84868256903     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb300025d     Document Type: Article

References (12)

1. Kodumal, S. J., Patel, K. G., Reid, R., Menzella, H. G., Welch, M., and Santi, D. V. (2004) Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster. Proc. Natl. Acad. Sci. U.S.A. 101, 15573-15578. (Pubitemid 39473529)
2. Liang, X., Peng, L., Tsvetanova, B., Li, K., Yang, J. P., Ho, T., Shirley, J., Xu, L., Potter, J., Kudlicki, W., Peterson, T., and Katzen, F. Recombination-based DNA assembly and mutagenesis methods for metabolic engineering. Methods Mol. Biol. 834, 93-109.
3. Farrar, K., and Donnison, I. S. (2007) Construction and screening of BAC libraries made from Brachypodium genomic DNA. Nat. Protoc. 2, 1661-1674.
4. Wingler, L. M., and Cornish, V. W. (2011) Reiterative Recombination for the in vivo assembly of libraries of multigene pathways. Proc. Natl. Acad. Sci. U.S.A. 108, 15135-15140.
5. Berlyn, M. K. (1998) Linkage map of Escherichia coli K-12, edition 10: The traditional map. Microbiol. Mol. Biol. Rev. 62, 814-984. (Pubitemid 28415555)
6. Gibson, D. G., Benders, G. A., Andrews-Pfannkoch, C., Denisova, E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., Merryman, C., Young, L., Noskov, V. N., Glass, J. I., Venter, J. C., Hutchison, C. A., 3rd, and Smith, H. O. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science 319, 1215-1220.
7. Schneider, D., Pohl, T., Walter, J., Dorner, K., Kohlstadt, M., Berger, A., Spehr, V., and Friedrich, T. (2008) Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Biochim. Biophys. Acta 1777, 735-739.
8. Godiska, R., Mead, D., Dhodda, V., Wu, C., Hochstein, R., Karsi, A., Usdin, K., Entezam, A., and Ravin, N. (2010) Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli. Nucleic Acids Res. 38, e88.
9. Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., and Mori, H. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: The Keio collection. Mol. Syst. Biol. 2, 2006 0008.
10. Roberts, R. J., Vincze, T., Posfai, J., and Macelis, D. (2010) REBASEa database for DNA restriction and modification: Enzymes, genes and genomes. Nucleic Acids Res. 38, D234-236.
11. Datsenko, K. A., and Wanner, B. L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97, 6640-6645. (Pubitemid 30412767)
12. Simon, R., Priefer, U., and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria. Nat. Biotech. 1, 784-791. (Pubitemid 14239484)