Labeling of active proteases in fresh-frozen tissues by topical application of quenched activity-based probes

Nature Protocols

Volumn 11, Issue 1, 2016, Pages 184-191

  Withana, Nimali P ^a   Garland, Megan ^a   Verdoes, Martijn ^a,b   Ofori, Leslie O ^a   Segal, Ehud ^a   Bogyo, Matthew ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CATHEPSIN B; CATHEPSIN L; CATHEPSIN S; CATHEPSIN X; PROTEINASE; FLUORESCENT DYE; PEPTIDE HYDROLASE;

ANTIBODY LABELING; ARTICLE; CHEMICAL LABELING; ENZYME ACTIVITY; EXPERIMENTAL DESIGN; FLUORESCENCE MICROSCOPY; HUMAN; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MOLECULAR PROBE; NUCLEAR MAGNETIC RESONANCE IMAGING; PRIORITY JOURNAL; QUENCHED ACTIVITY BASED PROBE; TUMOR VOLUME; CRYOPRESERVATION; ECONOMICS; ENZYME ASSAY; METABOLISM; PROCEDURES; STAINING; TEMPERATURE; TISSUE FIXATION;

CRYOPRESERVATION; ENZYME ASSAYS; FLUORESCENT DYES; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; PEPTIDE HYDROLASES; STAINING AND LABELING; TEMPERATURE; TISSUE FIXATION; TUMOR BURDEN;

EID: 84979862414     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.004     Document Type: Article

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