The natural flavone eupatorin induces cell cycle arrest at the G2/M phase and apoptosis in HeLa cells

Applied Biological Chemistry

Volumn 59, Issue 2, 2016, Pages 193-199

  Lee, Kyungho ^a   Hyun Lee, Da ^a   Jung, You Jung ^a   Shin, Soon Young ^a   Lee, Young Han ^a  

^a Konkuk University   (South Korea)

Author keywords

Apoptosis; Eupatorin; Flavone; G2 M arrest; P53

Indexed keywords

CELL CULTURE; CYTOTOXICITY; FLAVONOIDS; LANTHANUM COMPOUNDS;

ANTIPROLIFERATIVE ACTIVITIES; CANCER CELL LINES; CARCINOMA CELLS; CELL-CYCLE ARREST; CERVICAL CARCINOMA; CYTOTOXIC EFFECTS; EUPATORIN; FLAVONE; G2/M ARREST; HELA CELL;

CELL DEATH;

EID: 84977531322     PISSN: 24680834     EISSN: 24680842     Source Type: Journal    
DOI: 10.1007/s13765-016-0160-0     Document Type: Article

References (30)

1. Andree HA, Reutelingsperger CP, Hauptmann R, Hemker HC, Hermens WT, Willems GM (1990) Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers. J Biol Chem 265:4923–4928
2. Androutsopoulos V, Arroo RR, Hall JF, Surichan S, Potter GA (2008) Antiproliferative and cytostatic effects of the natural product eupatorin on MDA-MB-468 human breast cancer cells due to CYP1-mediated metabolism. Breast Cancer Res 10:R39
3. Bunz F, Hwang PM, Torrance C, Waldman T, Zhang Y, Dillehay L, Williams J, Lengauer C, Kinzler KW, Vogelstein B (1999) Disruption of p53 in human cancer cells alters the responses to therapeutic agents. J Clin Investig 104:263–269
4. Chipuk JE, Kuwana T, Bouchier-Hayes L, Droin NM, Newmeyer DD, Schuler M, Green DR (2004) Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. Science 303:1010–1014
5. Danial NN, Korsmeyer SJ (2004) Cell death: critical control points. Cell 116:205–219
6. Doleckova I, Rarova L, Gruz J, Vondrusova M, Strnad M, Krystof V (2012) Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves. Fitoterapia 83:1000–1007
7. Estevez S, Marrero MT, Quintana J, Estevez F (2014) Eupatorin-induced cell death in human leukemia cells is dependent on caspases and activates the mitogen-activated protein kinase pathway. PLoS ONE 9:e112536
8. Franzen CA, Amargo E, Todorovic V, Desai BV, Huda S, Mirzoeva S, Chiu K, Grzybowski BA, Chew TL, Green KJ, Pelling JC (2009) The chemopreventive bioflavonoid apigenin inhibits prostate cancer cell motility through the focal adhesion kinase/Src signaling mechanism. Cancer Prev Res (Phila) 2:830–841
9. Giaccia AJ, Kastan MB (1998) The complexity of p53 modulation: emerging patterns from divergent signals. Genes Dev 12:2973–2983
10. Jia LQ, Osada M, Ishioka C, Gamo M, Ikawa S, Suzuki T, Shimodaira H, Niitani T, Kudo T, Akiyama M, Kimura N, Matsuo M, Mizusawa H, Tanaka N, Koyama H, Namba M, Kanamaru R, Kuroki T (1997) Screening the p53 status of human cell lines using a yeast functional assay. Mol Carcinog 19:243–253
11. Lee WJ, Chen WK, Wang CJ, Lin WL, Tseng TH (2008) Apigenin inhibits HGF-promoted invasive growth and metastasis involving blocking PI3 K/Akt pathway and beta 4 integrin function in MDA-MB-231 breast cancer cells. Toxicol Appl Pharmacol 226:178–191
12. Levine AJ (1997) p53, the cellular gatekeeper for growth and division. Cell 88:323–331
13. Li ZD, Hu XW, Wang YT, Fang J (2009) Apigenin inhibits proliferation of ovarian cancer A2780 cells through Id1. FEBS Lett 583:1999–2003
14. Lin Y, Shi R, Wang X, Shen HM (2008) Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 8:634–646
15. Miyashita T, Reed JC (1995) Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80:293–299
16. Nagao T, Abe F, Kinjo J, Okabe H (2002) Antiproliferative constituents in plants 10. Flavones from the leaves of Lantana montevidensis Briq. and consideration of structure-activity relationship. Biol Pharm Bull 25:875–879
17. Nurse P (1990) Universal control mechanism regulating onset of M-phase. Nature 344:503–508
18. Oltvai ZN, Milliman CL, Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609–619
19. Resnitzky D, Gossen M, Bujard H, Reed SI (1994) Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system. Mol Cell Biol 14:1669–1679
20. Schinella GR, Giner RM, Recio MC, Mordujovich de Buschiazzo P, Rios JL, Manez S (1998) Anti-inflammatory effects of South American Tanacetum vulgare. J Pharm Pharmacol 50:1069–1074
21. Shin SY, Kim CG, Lim Y, Lee YH (2011) The ETS family transcription factor ELK-1 regulates induction of the cell cycle-regulatory gene p21(Waf1/Cip1) and the BAX gene in sodium arsenite-exposed human keratinocyte HaCaT cells. J Biol Chem 286:26860–26872
22. Shin SY, Kim JH, Lee JH, Lim Y, Lee YH (2012) 2′-Hydroxyflavanone induces apoptosis through Egr-1 involving expression of Bax, p21, and NAG-1 in colon cancer cells. Mol Nutr Food Res 56:761–774
23. Shin SY, Kim JH, Yoon H, Choi YK, Koh D, Lim Y, Lee YH (2013) Novel antimitotic activity of 2-hydroxy-4-methoxy-2′,3′-benzochalcone (HymnPro) through the inhibition of tubulin polymerization. J Agric Food Chem 61:12588–12597
24. Singh M, Kaur M, Silakari O (2014) Flavones: an important scaffold for medicinal chemistry. Eur J Med Chem 84:206–239
25. Stiegler P, Giordano A (2001) The family of retinoblastoma proteins. Crit Rev Eukaryot Gene Expr 11:59–76
26. Tezuka Y, Stampoulis P, Banskota AH, Awale S, Tran KQ, Saiki I, Kadota S (2000) Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chem Pharm Bull (Tokyo) 48:1711–1719
27. Thiel G, Cibelli G (2002) Regulation of life and death by the zinc finger transcription factor Egr-1. J Cell Physiol 193:287–292
28. Widel M, Lalik A, Krzywon A, Poleszczuk J, Fujarewicz K, Rzeszowska-Wolny J (2015) The different radiation response and radiation-induced bystander effects in colorectal carcinoma cells differing in p53 status. Mutat Res 778:61–70
29. Wu B, Zhang Q, Shen W, Zhu J (2008) Anti-proliferative and chemosensitizing effects of luteolin on human gastric cancer AGS cell line. Mol Cell Biochem 313:125–132
30. Zagurovskaya M, Shareef MM, Das A, Reeves A, Gupta S, Sudol M, Bedford MT, Prichard J, Mohiuddin M, Ahmed MM (2009) EGR-1 forms a complex with YAP-1 and upregulates Bax expression in irradiated prostate carcinoma cells. Oncogene 28:1121–1131