An in vitro compartmentalization-based method for the selection of bond-forming enzymes from large libraries

Biotechnology and Bioengineering

Volumn 113, Issue 8, 2016, Pages 1647-1657

  Gianella, Paul ^a   Snapp, Erik L ^a   Levy, Matthew ^a,b  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b 1301 Morris Park Ave   (United States)

Author keywords

biotin protein ligase (BirA); directed evolution; FACS; in vitro compartmentalization; microbead display; sortase A

Indexed keywords

BACTERIA; BIOLOGY; CELLS; CYTOLOGY; ENZYME ACTIVITY; LIBRARIES; NUCLEIC ACIDS; PROTEINS;

DIRECTED EVOLUTION; FACS; IN-VITRO; MICROBEADS; PROTEIN LIGASE; SORTASE A;

ENZYMES;

BIOTIN PROTEIN LIGASE; CALCIUM; LIGASE; PROTEIN VARIANT; SORTASE; SORTASE A; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM BINDING; CELL LABELING; CELL LYSATE; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME METABOLISM; EUKARYOTIC CELL; FLOW CYTOMETRY; IN VITRO COMPARTMENTALIZATION; IN VITRO STUDY; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ASSEMBLY; PROTEIN ENGINEERING; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS;

EID: 84977101038     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25939     Document Type: Article

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