Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry

Nature Protocols

Volumn 8, Issue 9, 2013, Pages 1808-1819

  Witte, Martin D ^a,d   Theile, Christopher S ^a   Wu, Tongfei ^b   Guimaraes, Carla P ^a   Blom, Annet E M ^a   Ploegh, Hidde L ^a,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; ANTIBODY; BISPECIFIC ANTIBODY; CHIMERIC PROTEIN; HYBRID PROTEIN; INTERLEUKIN 2; NANOBODY; PEPTIDE; RECOMBINANT PROTEIN; SORTASE; SORTASE A; UBIQUITIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL REACTION; CLICK CHEMISTRY; CONJUGATION; CONTROLLED STUDY; GENE FUSION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SYNTHESIS; TRANSPEPTIDATION;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CARBON; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLICK CHEMISTRY; CYSTEINE ENDOPEPTIDASES; NITROGEN; PEPTIDE FRAGMENTS; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS;

EID: 84883365413     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.103     Document Type: Article

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