Directed evolution of the substrate specificity of biotin ligase

Biotechnology and Bioengineering

Volumn 111, Issue 6, 2014, Pages 1071-1081

  Lu, Wei Cheng ^a   Levy, Matthew ^b   Kincaid, Rodney ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Biotin; Biotin protein ligase; Desthiobiotin; Emulsion; In vitro compartmentalization

Indexed keywords

BIOLOGY; EMULSIFICATION; EMULSIONS; ESCHERICHIA COLI; PEPTIDES; SUBSTRATES;

BIOTECHNOLOGICAL APPLICATIONS; BIOTIN; DESTHIOBIOTIN; DIRECTED EVOLUTION; IN-VITRO; PEPTIDE SUBSTRATES; PROTEIN LIGASE; SUBSTRATE SPECIFICITY;

COENZYMES;

BACTERIAL PROTEIN; BAP PEPTIDE; BIOTIN; BIOTIN DERIVATIVE; BIOTIN PROTEIN LIGASE; BIRA PROTEIN; DETHIOBIOTIN; DNA; LIGASE; METHIONINE; PEPTIDE DERIVATIVE; PROTEIN VARIANT; STREPTAVIDIN; THREONINE; UNCLASSIFIED DRUG; BIRA PROTEIN, E COLI; DESTHIOBIOTIN; ESCHERICHIA COLI PROTEIN; REPRESSOR PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; BIOTECHNOLOGY; BIOTINYLATION; CONJUGATION; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; DNA LIBRARY; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE AMPLIFICATION; IN VITRO STUDY; NONHUMAN; PHAGE DISPLAY; PROTEIN FOLDING; PROTEIN PURIFICATION; ANALOGS AND DERIVATIVES; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MISSENSE MUTATION;

ESCHERICHIA COLI;

AMINO ACID SUBSTITUTION; BIOTIN; CARBON-NITROGEN LIGASES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; MUTATION, MISSENSE; REPRESSOR PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84899651206     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25176     Document Type: Article

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