A method for detergent-free isolation of membrane proteins in their local lipid environment

Nature Protocols

Volumn 11, Issue 7, 2016, Pages 1149-1162

  Lee, Sarah C ^a   Knowles, Tim J ^a   Postis, Vincent L G ^b,c   Jamshad, Mohammed ^a   Parslow, Rosemary A ^a   Lin, Yu Pin ^a   Goldman, Adrian ^b,d   Sridhar, Pooja ^a   Overduin, Michael ^a,e   Muench, Stephen P ^b   Dafforn, Timothy R ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNIVERSITY OF LEEDS   (United Kingdom)

^c LEEDS METROPOLITAN UNIVERSITY   (United Kingdom)

^d UNIVERSITY OF HELSINKI   (Finland)

^e UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; COPOLYMER; HISTIDINE; LIPID; MALIC ACID; MEMBRANE PROTEIN; STYRENE; STYRENE MALEIC ACID COPOLYMER; STYRENE MALEIC ACID LIPID PARTICLE; STYRENE MALEIC ANHYDRIDE COPOLYMER; UNCLASSIFIED DRUG; ZIPA PROTEIN; ESCHERICHIA COLI PROTEIN; MALEIC ACID DERIVATIVE; MEMBRANE LIPID; POLYSTYRENE DERIVATIVE; STYRENE-MALEIC ACID POLYMER;

ARTICLE; BACTERIAL MEMBRANE; CIRCULAR DICHROISM; ELUTION; ENCAPSULATION; ESCHERICHIA COLI; HYDROLYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN ISOLATION; PROTEIN PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; SOLUBILIZATION; ULTRACENTRIFUGATION; CHEMISTRY; ISOLATION AND PURIFICATION; MOLECULAR MODEL; SOLUBILITY;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MALEATES; MEMBRANE LIPIDS; MEMBRANE PROTEINS; MODELS, MOLECULAR; POLYSTYRENES; SOLUBILITY;

EID: 84975853291     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.070     Document Type: Article

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