Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: The power of native nanodiscs

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 52, 2014, Pages 18607-18612

  Dörr, Jonas M ^a   Koorengevel, Martijn C ^a   Schäfer, Marre ^a   Prokofyev, Alexander V ^a,c   Scheidelaar, Stefan ^a   Van Der Cruijsen, Elwin A W ^a   Dafforn, Timothy R ^b   Baldus, Marc ^a   Killian, J Antoinette ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c UNIVERSITY OF TWENTE   (Netherlands)

Author keywords

Ion channels; Lipid protein interactions; Membrane protein solubilization; Nanodisc; Styrene maleic acid copolymer

Indexed keywords

CARDIOLIPIN; DETERGENT; ION CHANNEL; LIPID; MEMBRANE PROTEIN; NANODISC; PHOSPHATIDYLGLYCEROL; POTASSIUM CHANNEL; PROTEOLIPID; STYRENE MALEIC ANHYDRIDE COPOLYMER; TETRAMERIC POTASSIUM CHANNEL; TRYPTOPHAN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; LIPID BILAYER; NANOMATERIAL; PROKARYOTIC POTASSIUM CHANNEL; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL MEMBRANE; CHEMICAL ANALYSIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; ISOLATION PROCEDURE; LIPID BILAYER; LIPID COMPOSITION; MICELLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; THERMOSTABILITY; THIN LAYER CHROMATOGRAPHY; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; STREPTOMYCES LIVIDANS;

ESCHERICHIA COLI;

BACTERIAL PROTEINS; CARDIOLIPINS; ESCHERICHIA COLI; LIPID BILAYERS; NANOSTRUCTURES; PHOSPHATIDYLGLYCEROLS; POTASSIUM CHANNELS; RECOMBINANT PROTEINS; STREPTOMYCES LIVIDANS;

EID: 84924301475     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1416205112     Document Type: Article

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