메뉴 건너뛰기




Volumn 2, Issue 12, 2007, Pages 3239-3246

Preparation of macromolecular complexes for cryo-electron microscopy

Author keywords

[No Author keywords available]

Indexed keywords

CRYOPROTECTIVE AGENT; ICE;

EID: 39149091041     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.452     Document Type: Article
Times cited : (163)

References (24)
  • 1
    • 25444482114 scopus 로고    scopus 로고
    • The extracellular matrix and inflammation: Fibromodulin activates the classical pathway of complement by directly binding C1q
    • Sjoberg, A., Onnerfjord, P., Morgelin, M., Heinegard, D. & Blom, A.M. The extracellular matrix and inflammation: fibromodulin activates the classical pathway of complement by directly binding C1q. J. Biol. Chem. 280, 32301-32308 (2005).
    • (2005) J. Biol. Chem , vol.280 , pp. 32301-32308
    • Sjoberg, A.1    Onnerfjord, P.2    Morgelin, M.3    Heinegard, D.4    Blom, A.M.5
  • 2
    • 0035831269 scopus 로고    scopus 로고
    • Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit
    • Spahn, C.M. et al. Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit. Science 291,1959-1962 (2001).
    • (2001) Science , vol.291 , pp. 1959-1962
    • Spahn, C.M.1
  • 3
    • 0029003107 scopus 로고
    • The potential and limitations ofneutrons, electrons and X-rays for atomic resolution microscopy ofunstained biological molecules
    • Henderson, R. The potential and limitations ofneutrons, electrons and X-rays for atomic resolution microscopy ofunstained biological molecules. Q. Rev. Biophys. 28, 171-193 (1995).
    • (1995) Q. Rev. Biophys , vol.28 , pp. 171-193
    • Henderson, R.1
  • 5
    • 25844445494 scopus 로고    scopus 로고
    • Electron cryomicroscopy of single particles at subnanometer resolution
    • Jiang, W. & Ludtke, S.J. Electron cryomicroscopy of single particles at subnanometer resolution. Curr. Opin. Struct. Biol. 15, 571-577 (2005).
    • (2005) Curr. Opin. Struct. Biol , vol.15 , pp. 571-577
    • Jiang, W.1    Ludtke, S.J.2
  • 6
    • 34247560812 scopus 로고    scopus 로고
    • Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation
    • Taylor, D.J. et al. Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation. EMBO J 26, 2421-2431 (2007).
    • (2007) EMBO J , vol.26 , pp. 2421-2431
    • Taylor, D.J.1
  • 7
    • 0242407184 scopus 로고    scopus 로고
    • Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy
    • Valle, M. et al. Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy. Nat. Struct. Biol. 10, 899-906 (2003).
    • (2003) Nat. Struct. Biol , vol.10 , pp. 899-906
    • Valle, M.1
  • 8
    • 0033780164 scopus 로고    scopus 로고
    • Fitting atomic models into electron-microscopy maps
    • Rossmann, M.G. Fitting atomic models into electron-microscopy maps. Acta Crystallogr. D Biol. Crystallogr. 56, 1341-1349 (2000).
    • (2000) Acta Crystallogr. D Biol. Crystallogr , vol.56 , pp. 1341-1349
    • Rossmann, M.G.1
  • 9
    • 39449126462 scopus 로고    scopus 로고
    • Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai TEMs
    • Grassucci, R.A., Taylor, D. & Frank, J. Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai TEMs. Nat. Protoc. (in the press) doi: 10.1038/nprot.2007.474.
    • (2007) Nat. Protoc , pp. 474
    • Grassucci, R.A.1    Taylor, D.2    Frank, J.3
  • 10
    • 34249323754 scopus 로고    scopus 로고
    • RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors
    • Gao, H. et al. RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors. Cell 129, 929-941 (2007).
    • (2007) Cell , vol.129 , pp. 929-941
    • Gao, H.1
  • 11
    • 0035783162 scopus 로고    scopus 로고
    • Structures of unliganded and ATP-bound states of the Escherichia coli chaperonin GroEL by cryoelectron microscopy
    • Roseman, A.M., Ranson, N.A., Gowen, B., Fuller, S.D. & Saibil, H.R. Structures of unliganded and ATP-bound states of the Escherichia coli chaperonin GroEL by cryoelectron microscopy. J. Struct. Biol. 135, 115-125 (2001).
    • (2001) J. Struct. Biol , vol.135 , pp. 115-125
    • Roseman, A.M.1    Ranson, N.A.2    Gowen, B.3    Fuller, S.D.4    Saibil, H.R.5
  • 12
    • 0345561320 scopus 로고
    • Preparation of perforated films with predeterminable hole size distributions
    • Baumeister, W. & Seredynski, J. Preparation of perforated films with predeterminable hole size distributions. Micron 7, 49-54 (1976).
    • (1976) Micron , vol.7 , pp. 49-54
    • Baumeister, W.1    Seredynski, J.2
  • 13
    • 0020220722 scopus 로고
    • The mounting of macromolecules for electron microscopy with particular reference to surface phenomena and treatment of support films by glow discharge
    • (eds. Barrer, R. & Cosslett, V.E., Academic Press, London, New York
    • Dubochet, J., Groom, M. & Mueller-Neuteboom, S. The mounting of macromolecules for electron microscopy with particular reference to surface phenomena and treatment of support films by glow discharge. In Advances in Optical and Electron Microscopy (eds. Barrer, R. & Cosslett, V.E.) 107-135 (Academic Press, London, New York, 1982).
    • (1982) Advances in Optical and Electron Microscopy , pp. 107-135
    • Dubochet, J.1    Groom, M.2    Mueller-Neuteboom, S.3
  • 15
    • 0005383778 scopus 로고
    • The ultrastructure ofmacromolecularcomplexes studied with antibodies
    • (ed. Tesche, H., De Gruyter, Berlin
    • Stoffler, G. & Stoffler-Meilicke, M. The ultrastructure ofmacromolecularcomplexes studied with antibodies. In Modern Methods in Protein Chemistry (ed. Tesche, H.) 409-455 (De Gruyter, Berlin, 1983).
    • (1983) Modern Methods in Protein Chemistry , pp. 409-455
    • Stoffler, G.1    Stoffler-Meilicke, M.2
  • 16
    • 2342662152 scopus 로고    scopus 로고
    • Negative staining and image classification— powerful tools in modern electron microscopy
    • Ohi, M., Li, Y., Cheng, Y. & Walz, T. Negative staining and image classification— powerful tools in modern electron microscopy. Biol. Proced. Online 6, 23-34 (2004).
    • (2004) Biol. Proced. Online , vol.6 , pp. 23-34
    • Ohi, M.1    Li, Y.2    Cheng, Y.3    Walz, T.4
  • 19
    • 0036646535 scopus 로고    scopus 로고
    • Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process
    • Valle, M. et al. Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process. EMBO J 21, 3557-3567 (2002).
    • (2002) EMBO J , vol.21 , pp. 3557-3567
    • Valle, M.1
  • 20
    • 33845939047 scopus 로고    scopus 로고
    • Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization
    • Scheres, S.H. et al. Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization. Nat. Methods 4, 27-29 (2007).
    • (2007) Nat. Methods , vol.4 , pp. 27-29
    • Scheres, S.H.1
  • 21
    • 0038275890 scopus 로고    scopus 로고
    • Study of the structural dynamics of the E coli 70S ribosome using real-space refinement
    • Gao, H. et al. Study of the structural dynamics of the E coli 70S ribosome using real-space refinement. Cell 113, 789-801 (2003).
    • (2003) Cell , vol.113 , pp. 789-801
    • Gao, H.1
  • 22
    • 0025609474 scopus 로고
    • Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo-electron microscopy
    • Cyrklaff, M., Adrian, M. & Dubochet, J. Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo-electron microscopy. J. Electron Microsc. Tech. 16, 351-355 (1990).
    • (1990) J. Electron Microsc. Tech , vol.16 , pp. 351-355
    • Cyrklaff, M.1    Adrian, M.2    Dubochet, J.3
  • 23
    • 34147099837 scopus 로고    scopus 로고
    • Electron cryotomography sample preparation using the Vitrobot
    • Iancu, C.V. etal. Electron cryotomography sample preparation using the Vitrobot. Nat. Protoc. 1, 2813-2819 (2007).
    • (2007) Nat. Protoc , vol.1 , pp. 2813-2819
    • Iancu, C.V.1
  • 24
    • 0344983330 scopus 로고    scopus 로고
    • A second generation apparatus for time-resolved electron cryo-microscopy using stepper motors and electrospray
    • White, H.D., Thirumurugan, K., Walker, M.L. & Trinick, J. A second generation apparatus for time-resolved electron cryo-microscopy using stepper motors and electrospray. J. Struct. Biol. 144, 246-252 (2003).
    • (2003) J. Struct. Biol , vol.144 , pp. 246-252
    • White, H.D.1    Thirumurugan, K.2    Walker, M.L.3    Trinick, J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.