In vitro models for lung toxicology

Toxicology of the Lung, Fourth Edition

Volumn , Issue , 2005, Pages 107-150

  Allen, Corrie B ^a  

^a NONE   (United States)

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EID: 84974699795     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (261)

1. Taber, C.W., Taber’s Cyclopedic Medical Dictionary. 15th ed., Thomas, C., Ed., F.A. Davis Company, Philadelphia 1985.
2. Selgrade, M.K. et al., Evaluation of effects of ozone exposure on influenza infection in mice using several indicators of susceptibility. Fundam Appl Toxicol, 11, 169, 1988.
3. Kabel, J.R. et al., Longitudinal distribution of ozone absorption in the lung: comparison of nasal and oral quiet breathing. J Appl Physiol, 77, 2584, 1994.
4. Mautz, WJ., Exercising animal models in inhalation toxicology: interactions with ozone and formaldehyde. Environ Res, 92, 14, 2003.
5. Musi, B. et al., Effects of acute and continuous ozone (O3) exposure on activity/exploration and social behavior of CD-1 mice. Neurotoxicology, 15, 827, 1994.
6. Umezu, T. et al., Effect of ozone toxicity in the drinking behavior of rats. Arch Environ Health, 42, 58, 1987.
7. Umezu, T. et al., Effects of ozone and nitrogen dioxide on drinking and eating behaviors in mice. Environ Res, 61, 51, 1993.
8. Vincent, R. et al., Sensitivity of lungs of aging Fischer 344 rats to ozone: assessment by bronchoalveolar lavage. Am J Physiol, 271, L555, 1996.
9. Uhlson, C. et al., Oxidized phospholipids derived from ozone-treated lung surfactant extract reduce macrophage and epithelial cell viability. Chem Res Toxicol, 15, 896, 2002.
10. Squadrito, G.L. and Pryor, W.A., Oxidative chemistry of nitric oxide: the roles of superoxide, peroxynitrite, and carbon dioxide. Free Radic Biol Med, 25, 392, 1998.
11. Douglas, G.C. et al., HEPES buffer perfusate alters rabbit lung endothelial permeability. J Appl Physiol, 75, 1423, 1993.
12. Bowman, C.M. et al., HEPES may stimulate cultured endothelial cells to make growth-retarding oxygen metabolites. In Vitro Cell Dev Biol, 21, 140, 1985.
13. Hill, G.D. et al., Soluble ICAM-1, MCP-1, and MIP-2 protein secretion by rat pleural mesothelial cells following exposure to amosite asbestos. Exp Lung Res, 29, 277, 2003.
14. Huang, S.L. et al., Contribution of endotoxin in macrophage cytokine response to ambient particles in vitro. J Toxicol Environ Health A, 65, 1261, 2002.
15. Cottet-Emard, J.M. et al., Long-term exposure to ozone alters peripheral and central catecholamine activity in rats. Pflugers Arch, 433, 744, 1997.
16. Peden, D.B. and Dailey, L., Modulation of mast cell functions by in vitro ozone exposure. Am J Physiol, 268, L902, 1995.
17. Gross, K.B. et al., The effect of ozone inhalation on metabolic functioning of vascular endothelium and on ventilatory function. Toxicol Appl Pharmacol, 109, 336, 1991.
18. Block, E.R. and Stalcup, S.A., Depression of serotonin uptake by cultured endothelial cells exposed to high O2 tension. J Appl Physiol, 50, 1212, 1981.
19. Mustafa, M.G. and Cross, C.E., Effects of short-term ozone exposure on lung mitochondrial oxidative and energy metabolism. Arch Biochem Biophys, 162, 585, 1974.
20. Mustafa, M.G. et al., Ozone interaction with rodent lung. II. Effects on oxygen consumption of mitochondria. J Lab Clin Med, 82, 357, 1973.
21. Zychlinski, L. et al., Age-related difference in bioenergetics of lung and heart mitochondrial from rats exposed to ozone. J Biochem Toxicol, 4, 251, 1989.
22. Mustafa, M.G., Augmentation of mitochondrial oxidative metabolism in lung tissue during recovery of animals from acute ozone exposure. Arch Biochem Biophys, 165, 531, 1974.
23. Bassett, DJ. and Bowen-Kelly, E., Rat lung metabolism after 3 days of continuous exposure to 0.6 ppm ozone. Am J Physiol, 250, E131, 1986.
24. Beach, A.C. and Harmon, HJ., Additive effects and potential inhibitory mechanism of some common aromatic pollutants on in vitro mitochondrial respiration. J Biochem Toxicol, 7, 155, 1992.
25. Morin, J.P. et al., Development of a new in vitro system for continuous in vitro exposure of lung tissue to complex atmospheres: application to diesel exhaust toxicology. Cell Biol Toxicol, 15, 143, 1999.
26. Castranova, V. et al., Toxicity of metal ions to alveolar macrophages. Am J Ind Med, 1, 349, 1980.
27. Yoon, B.I. et al., Aryl hydrocarbon receptor mediates benzene-induced hema-totoxicity. Toxicol Sci, 70, 150, 2002.
28. Santini, R.P. et al., Regulation of Cyp1a1 induction by dioxin as a function of cell cycle phase. J Pharmacol Exp Ther, 299, 718, 2001.
29. Gardner, D.E., Alterations in macrophage functions by environmental chemicals. Environ Health Perspect, 55, 343, 1984.
30. Girard, D., Activation of human polymorphonuclear neutrophils by environmental contaminants. Rev Environ Health, 18, 75, 2003.
31. Shigehara, T. et al., Sulfite induces adherence of polymorphonuclear neutrophils to immobilized fibrinogen through activation of Mac-1 beta2-integrin (CD11b/CD18). Life Sci, 70, 2225, 2002.
32. Pearson, A.C. and Bhalla, D.K., Effects of ozone on macrophage adhesion in vitro and epithelial and inflammatory responses in vivo: the role of cytokines. J Toxicol Environ Health, 50, 143, 1997.
33. Driscoll, K.E. and Schlesinger, R.B., Alveolar macrophage-stimulated neutrophil and monocyte migration: effects of in vitro ozone exposure. Toxicol Appl Pharmacol, 93, 312, 1988.
34. Dong, W. et al., Altered alveolar macrophage function in calorie-restricted rats. Am J Respir Cell Mol Biol, 19, 462, 1998.
35. Sherwood, R.L. et al., Effects of inhaled hexachlorobenzene aerosols on rat pulmonary host defenses. Toxicol Ind Health, 5, 451, 1989.
36. Becker, S. et al., Differential particulate air pollution induced oxidant stress in human granulocytes, monocytes and alveolar macrophages. Toxicol In Vitro, 16, 209, 2002.
37. Nguyen, H. et al., Cigarette smoke impairs neutrophil respiratory burst activation by aldehyde-induced thiol modifications. Toxicology, 160, 207, 2001.
38. Pelletier, M. et al., Activation of human neutrophils in vitro and dieldrin-induced neutrophilic inflammation in vivo. J Leukoc Biol, 70, 367, 2001.
39. Jakab, G.J., Modulation of pulmonary defense mechanisms by acute exposures to nitrogen dioxide. Environ Res, 42, 215, 1987.
40. Aranyi, C. et al., Effects of toluene inhalation on pulmonary host defenses of mice. Toxicol Lett, 25, 103, 1985.
41. Johnson, J.D. et al., Activation of alveolar macrophages after lower respiratory tract infection. J Immunol, 115, 80, 1975.
42. Becker, S. et al., Modulation of human alveolar macrophage properties by ozone exposure in vitro. Toxicol Appl Pharmacol, 110, 403, 1991.
43. Soukup, J.M. and Becker, S., Human alveolar macrophage responses to air pollution particulates are associated with insoluble components of coarse material, including particulate endotoxin. Toxicol Appl Pharmacol, 171, 20, 2001.
44. Mukae, H. et al., Phagocytosis of particulate air pollutants by human alveolar macrophages stimulates the bone marrow. Am J Physiol Lung Cell Mol Physiol, 279, L924, 2000.
45. van Eeden, S.F. et al., Cytokines involved in the systemic inflammatory response induced by exposure to particulate matter air pollutants (PM10). Am J Respir Crit Care Med, 164, 826, 2001.
46. Pelletier, M. et al., Activation of human neutrophils by the air pollutant sodium sulfite Na(2)SO(3): comparison with immature promyelocytic HL-60 and DMSO-differentiated HL-60 cells reveals that Na(2)SO(3) is a neutrophil but not a HL-60 cell agonist. Clin Immunol, 96, 131, 2000.
47. Thomassen, M.J. et al., Regulation of human alveolar macrophage inflammatory cytokine production by interleukin-10. Clin Immunol Immunopathol, 80, 321, 1996.
48. Curi, T.C. et al., Percentage of phagocytosis, production of O2.-, H2O2 and NO, and antioxidant enzyme activities of rat neutrophils in culture. Cell Biochem Funct, 16, 43, 1998.
49. Aljandali, A. et al., Asbestos causes apoptosis in alveolar epithelial cells: role of iron-induced free radicals. J Lab Clin Med, 137, 330, 2001.
50. Le Prieur, E. et al., Toxicity of diesel engine exhausts in an in vitro model of lung slices in biphasic organotypic culture: induction of a proinflammatory and apoptotic response. Arch Toxicol, 74, 460, 2000.
51. Holian, A. et al., Urban particle-induced apoptosis and phenotype shifts in human alveolar macrophages. Environ Health Perspect, 106, 127, 1998.
52. Zhao, X.H. et al., Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells. Toxicol Lett, 122, 103, 2001.
53. Panduri, V. et al., Mitochondrial-derived free radicals mediate asbestos-induced alveolar epithelial cell apoptosis. AmJ Physiol Lung Cell Mol Physiol, 286, L1220, 2004.
54. Chao, S.K. et al., Cell surface regulation of silica-induced apoptosis by the SR-A scavenger receptor in a murine lung macrophage cell line (MH-S). Toxicol Appl Pharmacol, 174, 10, 2001.
55. Duke, R.C. and Cohen, J.J., IL-2 addiction: withdrawal of growth factor activates a suicide program in dependent T cells. Lymphokine Res, 5, 289, 1986.
56. Iyer, R. et al., Silica-induced apoptosis mediated via scavenger receptor in human alveolar macrophages. Toxicol Appl Pharmacol, 141, 84, 1996.
57. Knebel, J.W. et al., Exposure of human lung cells to native diesel motor exhaust—development of an optimized in vitro test strategy. Toxicol In Vitro, 16, 185, 2002.
58. Allen, C.B., An automated system for exposure of cultured cells and other materials to ozone. Inhal Toxicol, 15, 1039, 2003.
59. Aufderheide, M. et al., An improved in vitro model for testing the pulmonary toxicity of complex mixtures such as cigarette smoke. Exp Toxicol Pathol, 55, 51, 2003.
60. Bouthillier, L. et al., Acute effects of inhaled urban particles and ozone: lung morphology, macrophage activity, and plasma endothelin-1. Am J Pathol, 153, 1873, 1998.
61. Sioutas, C. et al., A technique to expose animals to concentrated fine ambient aerosols. Environ Health Perspect, 103, 172, 1995.
62. Ghio, AJ. and Huang, Y.C., Exposure to concentrated ambient particles (CAPs): a review. Inhal Toxicol, 16, 53, 2004.
63. Demokritou, P. et al., Development of a high-volume concentrated ambient particles system (CAPS) for human and animal inhalation toxicological studies. Inhal Toxicol, 15, 111, 2003.
64. Kodavanti, U.P. et al., Variable pulmonary responses from exposure to concentrated ambient air particles in a rat model of bronchitis. Toxicol Sci, 54, 441, 2000.
65. Clarke, R.W. et al., Urban air particulate inhalation alters pulmonary function and induces pulmonary inflammation in a rodent model of chronic bronchitis. Inhal Toxicol, 11, 637, 1999.
66. Wellenius, G.A. et al., Inhalation of concentrated ambient air particles exacerbates myocardial ischemia in conscious dogs. Environ Health Perspect, 111, 402, 2003.
67. Ghio, AJ. et al., Concentrated ambient air particles induce mild pulmonary inflammation in healthy human volunteers. Am J Respir Crit Care Med, 162, 981, 2000.
68. Devlin, R.B. et al., Elderly humans exposed to concentrated air pollution particles have decreased heart rate variability. Eur Respir J Suppl, 40, 76s, 2003.
69. Harder, S.D. et al., Inhalation of PM2.5 does not modulate host defense or immune parameters in blood or lung of normal human subjects. Environ Health Perspect, 109(Suppl. 4), 599, 2001.
70. Gong, H., Jr. et al., Controlled exposures of healthy and asthmatic volunteers to concentrated ambient particles in metropolitan Los Angeles. Res Rep Health Eff Inst, 1, 2003.
71. Holgate, S.T. et al., Health effects of acute exposure to air pollution. Part II: Healthy subjects exposed to concentrated ambient particles. Res Rep Health Eff Inst, 31, 2003.
72. Ghio, AJ. et al., Exposure to concentrated ambient air particles alters hematologic indices in humans. Inhal Toxicol, 15, 1465, 2003.
73. Imrich, A. et al., Insoluble components of concentrated air particles mediate alveolar macrophage responses in vitro. Toxicol Appl Pharmacol, 167, 140, 2000.
74. Imrich, A. et al., Lipopolysaccharide priming amplifies lung macrophage tumor necrosis factor production in response to air particles. Toxicol Appl Pharmacol, 159, 117, 1999.
75. Ning, Y. et al., Particle-epithelial interaction: effect of priming and bystander neutrophils on interleukin-8 release. Am J Respir Cell Mol Biol, 30, 744, 2004.
76. Stringer, B. et al., Lung epithelial cell (A549) interaction with unopsonized environmental particulates: quantitation of particle-specific binding and IL-8 production. Exp Lung Res, 22, 495, 1996.
77. Freshney, R.I., Culture of Animal Cells: a Manual of Basic Technique. 4th ed., John Wiley & Sons, New York, 2000.
78. McDonald, RJ. and Usachencko, J., Neutrophils injure bronchial epithelium after ozone exposure. Inflammation, 23, 63, 1999.
79. Koike, E. et al., Effect of ozone exposure on alveolar macrophage-mediated immunosuppressive activity in rats. Toxicol Sci, 41, 217, 1998.
80. Koike, E. et al., Mechanisms of ozone-induced inhibitory effect of bronchoal-veolar lavage fluid on alveolar macrophage-mediated immunosuppressive activity in rats. J Leukoc Biol, 66, 75, 1999.
81. Hamilton, R.F., Jr. et al., Silica and PM1648 modify human alveolar macrophage antigen-presenting cell activity in vitro. J Environ Pathol Toxicol Oncol, 20(Suppl. 1), 75, 2001.
82. Becker, S. and Soukup, J.M., Decreased CD11b expression, phagocytosis, and oxidative burst in urban particulate pollution-exposed human monocytes and alveolar macrophages. J Toxicol Environ Health A, 55, 455, 1998.
83. Hadnagy, W. and Seemayer, N.H., Inhibition of phagocytosis of human macrophages induced by airborne particulates. Toxicol Lett, 72, 23, 1994.
84. Pendino, KJ. et al., Stimulation of nitric oxide production in rat lung lavage cells by anti-Mac-1beta antibody: effects of ozone inhalation. Am J Respir Cell Mol Biol, 14, 327, 1996.
85. Bhalla, D.K., Alteration of alveolar macrophage chemotaxis, cell adhesion, and cell adhesion molecules following ozone exposure of rats. J Cell Physiol, 169, 429, 1996.
86. Bermudez, E. et al., DNA strand breaks caused by exposure to ozone and nitrogen dioxide. Environ Res, 81, 72, 1999.
87. Bermudez, E., Detection of poly(ADP-ribose) synthetase activity in alveolar macrophages of rats exposed to nitrogen dioxide and ozone. Inhal Toxicol, 13, 69, 2001.
88. Becker, S. et al., Stimulation of human and rat alveolar macrophages by urban air particulates: effects on oxidant radical generation and cytokine production. Toxicol Appl Pharmacol, 141, 637, 1996.
89. Driscoll, K.E. et al., Alveolar macrophage cytokine and growth factor production in a rat model of crocidolite-induced pulmonary inflammation and fibrosis. J Toxicol Environ Health, 46, 155, 1995.
90. Devlin, R.B. et al., Ozone-induced release of cytokines and fibronectin by alveolar macrophages and airway epithelial cells. Am J Physiol, 266, L612, 1994.
91. Bhalla, D.K. et al., Modification of macrophage adhesion by ozone: role of cytokines and cell adhesion molecules. Ann N Y Acad Sci, 796, 38, 1996.
92. Ishii, Y. et al., Rat alveolar macrophage cytokine production and regulation of neutrophil recruitment following acute ozone exposure. Toxicol Appl Pharmacol, 147, 214, 1997.
93. Henderson, R.F., in Toxicology of the Lung (Gardner, D.E., Crapo, J.D., and Massaro, EJ., Eds.), 1st ed., Raven Press, New York, 1988, p. 239.
94. Henderson, R.F. et al., Comparative study of bronchoalveolar lavage fluid: effect of species, age, and method of lavage. Exp Lung Res, 13, 329, 1987.
95. Shapiro, H.M., Practical Flow Cytometry. 4th ed., Wiley-Liss, Hoboken, NJ, 2003.
96. Lorsbach, R.B. et al., Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J Biol Chem, 268, 1908, 1993.
97. Zeidler, P.C. et al., Response of alveolar macrophages from inducible nitric oxide synthase knockout or wild-type mice to an in vitro lipopolysaccharide or silica exposure. J Toxicol Environ Health A, 66, 995, 2003.
98. Lundborg, M. et al., Human alveolar macrophage phagocytic function is impaired by aggregates of ultrafine carbon particles. Environ Res, 86, 244, 2001.
99. Dorger, M. et al., Comparison of the phagocytic response of rat and hamster alveolar macrophages to man-made vitreous fibers in vitro. Hum Exp Toxicol, 19, 635, 2000.
100. Quinlan, T.R. et al., Mechanisms of asbestos-induced nitric oxide production by rat alveolar macrophages in inhalation and in vitro models. Free Radic Biol Med, 24, 778, 1998.
101. Dandrea, T. et al., Differential inhibition of inflammatory cytokine release from cultured alveolar macrophages from smokers and non-smokers by NO2. Hum Exp Toxicol, 16, 577, 1997.
102. Helmke, RJ. et al., From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell Dev Biol, 23, 567, 1987.
103. Palleroni, A.V. et al., Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int J Cancer, 49, 296, 1991.
104. Raschke, W.C. et al., Functional macrophage cell lines transformed by Abelson leukemia virus. Cell, 15, 261, 1978.
105. Cheek, J.M. et al., Neutrophils enhance removal of ozone-injured alveolar epithelial cells in vitro. Am J Physiol, 269, L527, 1995.
106. Mogel, M. et al., A new coculture-system of bronchial epithelial and endothelial cells as a model for studying ozone effects on airway tissue. Toxicol Lett, 96–97, 25, 1998.
107. Fujii, T. et al., Particulate matter induces cytokine expression in human bronchial epithelial cells. Am J Respir Cell Mol Biol, 25, 265, 2001.
108. Lechner, J.F. et al., Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum. Differentiation, 25, 229, 1984.
109. Beckmann, J.D. et al., Serum-free culture of fractionated bovine bronchial epithelial cells. In Vitro Cell Dev Biol, 28A, 39, 1992.
110. Smith, M.D. et al., Techniques for measurement of oxygen consumption rates of hepatocytes during attachment and post-attachment. Int J Artif Organs, 19, 36, 1996.
111. Bruttig, S.P. and Joyner, W.L., Metabolic characteristics of cells cultured from human umbilical blood vessels: comparison with 3T3 fibroblasts. J Cell Physiol, 116, 173, 1983.
112. Jorjani, P. and Ozturk, S.S., Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines. Biotechnol Bioeng, 64, 349, 1999.
113. Martin, M. et al., Energetic and morphological plasticity of C6 glioma cells grown on 3-D support; effect of transient glutamine deprivation. J Bioenerg Biomembr, 30, 565, 1998.
114. Kittlick, P.D. and Neupert, G., Hypoxia in fibroblast cultures changes in glucose consumption by 5 oxygen concentration and reduced pH. Exp Pathol, 10, 109, 1975.
115. Fiorani, M. et al., Cell density-dependent regulation of ATP levels during the growth cycle of cultured Chinese hamster ovary cells. Biochem Mol Biol Int, 32, 251, 1994.
116. Bereiter-Hahn, J. et al., Dependence of energy metabolism on the density of cells in culture. Cell Struct Funct, 23, 85, 1998.
117. Sung, S.S. et al., Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages. J Biol Chem, 260, 13442, 1985.
118. Zupke, C. et al., Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol, 44, 27, 1995.
119. Grigoriev, V.G. et al., Senescence and cell density of human diploid fibroblasts influence metabolism of insulin-like growth factor binding proteins. J Cell Physiol, 160, 203, 1994.
120. Kenagy, R. et al., Metabolism of low density lipoprotein by bovine endothelial cells as a function of cell density. Arteriosclerosis, 4, 365, 1984.
121. Knedler, A. and Ham, R.G., Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell Dev Biol, 23, 481, 1987.
122. Stanners, C.P. et al., Two types of ribosome in mouse-hamster hybrid cells. Nat New Biol, 230, 52, 1971.
123. Stewart, C.C., Nutrient utilization by peritoneal exudate cells. J Reticuloendothel Soc, 14, 332, 1973.
124. van der Zeijst, B.A. et al., Proliferative capacity of mouse peritoneal macrophages in vitro. J Exp Med, 147, 1253, 1978.
125. Reppun, T.S. et al., Isokinetic separation and characterization of mouse pulmonary alveolar colony-forming cells. J Reticuloendothel Soc, 25, 379, 1979.
126. Lin, H.S. et al., Effects of hydrocortisone acetate on pulmonary alveolar macrophage colony-forming cells. Am Rev Respir Dis, 125, 712, 1982.
127. Lin, H.S. and Hsu, S., Effects of dose rate and dose fractionation of irradiation on pulmonary alveolar macrophage colony-forming cells. Radiat Res, 103, 260, 1985.
128. Lin, H.S. et al., Clonal growth of hamster free alveolar cells in soft agar. J Exp Med, 142, 877, 1975.
129. Lechner, J.F. and LaVeck, M.A., A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J Tissue Cult Methods, 9, 43, 1985.
130. Metzen, E. et al., Pericellular PO2 and O2 consumption in monolayer cell cultures. Respir Physiol, 100, 101, 1995.
131. Allen, C.B. et al., Limitations to oxygen diffusion and equilibration in in vitro cell exposure systems in hyperoxia and hypoxia. Am J Physiol Lung Cell Mol Physiol, 281, L1021, 2001.
132. Dumler, K. et al., The effects of ozone exposure on lactate dehydrogenase release from human and primate respiratory epithelial cells. Toxicol Lett, 70, 203, 1994.
133. McManus, M.S. et al., Human nasal epithelium: characterization and effects of in vitro exposure to sulfur dioxide. Exp Lung Res, 15, 849, 1989.
134. Valentine, R., An in vitro system for exposure of lung cells to gases: effects of ozone on rat macrophages. J Toxicol Environ Health, 16, 115, 1985.
135. Tarkington, B.K. et al., Ozone exposure of cultured cells and tissues. Methods Enzymol, 234, 257, 1994.
136. Tarkington, B.K. et al., In vitro exposure of tracheobronchial epithelial cells and of tracheal explants to ozone. Toxicology, 88, 51, 1994.
137. Baker, F.D. and Tumasonis, C.F., Modified roller drum apparatus for analyzing effects of pollutant gases on tissue culture systems. Atmos Environ, 5, 891, 1971.
138. Bolton, D.C. et al., An in vitro system for studying the effects of ozone on mammalian cell cultures and viruses. Environ Res, 27, 466, 1982.
139. Lee, J.G. et al., The use of the single cell gel electrophoresis assay in detecting DNA single strand breaks in lung cells in vitro. Toxicol Appl Pharmacol, 141, 195, 1996.
140. Aufderheide, M. et al., Novel approaches for studying pulmonary toxicity in vitro. Toxicol Lett, 140–141, 205, 2003.
141. Umstead, T.M. et al., In vitro exposure of proteins to ozone. Toxicol Mech Meth, 12, 1, 2002.
142. Ritter, D. et al., Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette mainstream smoke. Inhal Toxicol, 15, 67, 2003.
143. Aufderheide, M. et al., A method for the in vitro exposure of human cells to environmental and complex gaseous mixtures: application to various types of atmosphere. Altern Lab Anim, 30, 433, 2002.
144. Aufderheide, M. et al., A method for in vitro analysis of the biological activity of complex mixtures such as sidestream cigarette smoke. Exp Toxicol Pathol, 53, 141, 2001.
145. Ritter, D. et al., In vitro exposure of isolated cells to native gaseous compounds —development and validation of an optimized system for human lung cells. Exp Toxicol Pathol, 53, 373, 2001.
146. Fisher, G.L. and Placke, M.E., in Toxicology of the Lung, Gardner, D.E., Crapo, J.D., and Massaro, EJ., Eds., 1st ed., Raven Press, New York, 1988, p. 285.
147. Gelfand, A.S. et al., Effect of aspiration of milk on mechanisms of neural control in the airways of developing rabbits. Pediatr Pulmonol, 23, 198, 1997.
148. Roux, E. et al., Human isolated airway contraction: interaction between air pollutants and passive sensitization. Am J Respir Crit Care Med, 160, 439, 1999.
149. Ben-Jebria, A. et al., Effect of in vitro nitrogen dioxide exposure on human bronchial smooth muscle response. Am Rev Respir Dis, 146, 378, 1992.
150. Walters, E.H. et al., The responsiveness of airway smooth muscle in vitro from dogs with airway hyper-responsiveness in vivo. Clin Sci (Lond), 71, 605, 1986.
151. Montano, L.M. et al., Effect of ozone exposure in vivo on response of bronchial rings in vitro: role of intracellular Ca2+. J Appl Physiol, 75, 1315, 1993.
152. Takata, S. et al., Ozone exposure suppresses epithelium-dependent relaxation in feline airway. Lung, 173, 47, 1995.
153. Yoshida, M. et al., Ozone exposure may enhance airway smooth muscle contraction by increasing Ca(2+) refilling of sarcoplasmic reticulum in guinea pig. Pulm Pharmacol Ther, 15, 111, 2002.
154. Chitano, P. et al., In vitro exposure to nitrogen dioxide (NO2) does not alter bronchial smooth muscle responsiveness in ovalbumin-sensitized guinea-pigs. Pulm Pharmacol, 7, 251, 1994.
155. Murlas, C.G. et al., O3-induced mucosa-linked airway muscle hyperresponsiveness in the guinea pig. J Appl Physiol, 69, 7, 1990.
156. Sommer, B. et al., Effect of different ozone concentrations on the neurogenic contraction and relaxation of guinea pig airways. Fundam Clin Pharmacol, 11, 501, 1997.
157. van Hoof, I.H. et al., Changes in receptor function by oxidative stress in guinea pig tracheal smooth muscle. Cent Eur J Public Health, 4(Suppl. 3), 1996.
158. Roux, E. et al., Human and rat airway smooth muscle responsiveness after ozone exposure in vitro. Am J Physiol, 271, L631, 1996.
159. Christ, M.J. et al., Amiloride-induced contraction of isolated guinea pig, mouse, and human fetal airways. Am J Physiol, 274, R209, 1998.
160. Garssen, J. et al., An isometric method to study respiratory smooth muscle responses in mice. J Pharmacol Methods, 24, 209, 1990.
161. Iwamoto, L.M. et al., Loop diuretics and in vitro relaxation of human fetal and newborn mouse airways. Pediatr Res, 50, 273, 2001.
162. Justice, J.P. et al., IL-10 gene knockout attenuates allergen-induced airway hyperresponsiveness in C57BL/6 mice. Am J Physiol Lung Cell Mol Physiol, 280, L363, 2001.
163. Sipahi, E.Y. et al., Nitric oxide and prostanoid-dependent relaxation induced by angiotensin II in the isolated precontracted mouse tracheal muscle and the role of potassium channels. Pharmacol Res, 42, 69, 2000.
164. Chitano, P. et al., Isotonic smooth muscle response in human bronchi exposed in vitro to nitrogen dioxide. Eur Respir J, 9, 2294, 1996.
165. Michielsen, C. et al., The environmental pollutant hexachlorobenzene causes eosinophilic and granulomatous inflammation and in vitro airways hyperreactivity in the Brown Norway rat. Arch Toxicol, 76, 236, 2002.
166. Chitano, P. et al., In-vitro exposure of guinea pig main bronchi to 2.5 ppm of nitrogen dioxide does not alter airway smooth muscle response. Respir Med, 89, 323, 1995.
167. Jain, B. et al., Modulation of airway epithelial cell ciliary beat frequency by nitric oxide. Biochem Biophys Res Commn, 191, 83, 1993.
168. Uzlaner, N. and Priel, Z., Interplay between the NO pathway and elevated [Ca2+]i enhances ciliary activity in rabbit trachea. J Physiol, 516 (Pt. 1), 179, 1999.
169. Sisson, J.H. et al., Acetaldehyde-mediated cilia dysfunction in bovine bronchial epithelial cells. Am J Physiol, 260, L29, 1991.
170. Grose, E.C. et al., Pulmonary host defense responses to inhalation of sulfuric acid and ozone. J Toxicol Environ Health, 10, 351, 1982.
171. Grose, E.C. et al., Response of ciliated epithelium to ozone and sulfuric acid. Environ Res, 22, 377, 1980.
172. Sakai, N. et al., Inhibitory effect on sulfur dioxide on ciliary motility in rabbit tracheal epithelium and its prevention by intracellular cyclic AMP. Nihon Kyobu Shikkan Gakkai Zasshi, 31, 733, 1993.
173. Tamaoki, J. et al., Effect of azelastine on sulphur dioxide induced impairment of ciliary motility in airway epithelium. Thorax, 48, 542, 1993.
174. Adalis, D. et al., Toxic effects of cadmium on ciliary activity using a tracheal ring model system. Environ Res, 13, 111, 1977.
175. Adalis, D. et al., Cytotoxic effects of nickel on ciliated epithelium. Am Rev Respir Dis, 118, 347, 1978.
176. Korngreen, A. and Priel, Z., Purinergic stimulation of rabbit ciliated airway epithelia: control by multiple calcium sources. J Physiol, 497(Pt. 1), 53, 1996.
177. Sakai, A. et al., Nitric oxide modulation of Ca2+ responses in cow tracheal epithelium. Eur J Pharmacol, 291, 375, 1995.
178. Jain, B. et al., TNF-alpha and IL-1 beta upregulate nitric oxide-dependent ciliary motility in bovine airway epithelium. Am J Physiol, 268, L911, 1995.
179. Saxton, R.E. et al., Dose response of human tumor cells to rhodamine 123 and laser phototherapy. Laryngoscope, 104, 1013, 1994.
180. Petrat, F. et al., NAD(P)H, a primary target of 1O2 in mitochondria of intact cells. J Biol Chem, 278, 3298, 2003.2
181. Krishan, A. et al., Drug retention, efflux, and resistance in tumor cells. Cytometry, 29, 279, 1997.
182. Tang-Wai, D.F. et al., Human (MDR1) and mouse (mdr1, mdr3) P-glycoproteins can be distinguished by their respective drug resistance profiles and sensitivity to modulators. Biochemistry (Mosc), 34, 32, 1995.
183. Altenberg, G.A. et al., Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane. Proc Natl Acad Sci USA, 91, 4654, 1994.
184. Nare, B. et al., Characterization of rhodamine 123 binding to P-glycoprotein in human multidrug-resistant cells. Mol Pharmacol, 45, 1145, 1994.
185. Ludescher, C. et al., Rapid functional assay for the detection of multidrug-resistant cells using the fluorescent dye rhodamine 123. Blood, 78, 1385, 1991.
186. Kessel, D. et al., Characterization of multidrug resistance by fluorescent dyes. Cancer Res, 51, 4665, 1991.
187. Lautier, D. et al., Detection of human leukemia cells with multidrug-resistance phenotype using multilabeling with fluorescent dyes. Anticancer Res, 13, 1557, 1993.
188. Lahmy, S. et al., Identification of multi-drug resistant cells in sensitive Friend leukemia cells by quantitative videomicrofluorimetry. Cell Biochem Funct, 10, 9, 1992.
189. Frey, T. et al., Dyes providing increased sensitivity in flow-cytometric dye-efflux assays for multidrug resistance. Cytometry, 20, 218, 1995.
190. Olson, D.P. et al., Detection of MRP functional activity: calcein AM but not BCECF AM as a multidrug resistance-related Protein (MRP1) substrate. Cytometry, 46, 105, 2001.
191. Nelson, EJ. et al., Fluorescence methods to assess multidrug resistance in individual cells. Cancer Chemother Pharmacol, 42, 292, 1998.
192. Brezden, C.B. et al., Constitutive expression of P-glycoprotein as a determinant of loading with fluorescent calcium probes. Cytometry, 17, 343, 1994.
193. Blum, J.L. and Wicha, M.S., Role of the cytoskeleton in laminin induced mammary gene expression. J Cell Physiol, 135, 13, 1988.
194. Li, M.L. et al., Influence of a reconstituted basement membrane and its com ponents on casein gene expression and secretion in mouse mammary epithelial cells. Proc Natl Acad Sci USA, 84, 136, 1987.
195. Folkman, J. and Moscona, A., Role of cell shape in growth control. Nature, 273, 345, 1978.
196. Streuli, C.H. et al., Control of mammary epithelial differentiation: basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity. J Cell Biol, 115, 1383, 1991.
197. Lin, C.Q. and Bissell, MJ., Multi-faceted regulation of cell differentiation by extracellular matrix [see comments]. FASEB J, 7, 737, 1993.
198. Ingber, D.E. et al., Cellular tensegrity: exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis. Int Rev Cytol, 150, 173, 1994.
199. Wang, N. and Ingber, D.E., Control of cytoskeletal mechanics by extracellular matrix, cell shape, and mechanical tension. Biophys J, 66, 2181, 1994.
200. Ingber, D.E. et al., Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis. J Biomech, 28, 1471, 1995.
201. Chen, C.S. et al., Geometric control of cell life and death. Science, 276, 1425, 1997.
202. Ingber, D.E., The architecture of life. Sci Am, 48, 1998.
203. Hays, A.M. et al., Correlation between in vivo and in vitro pulmonary responses to jet propulsion fuel-8 using precision-cut lung slices and a dynamic organ culture system. Toxicol Pathol, 31, 200, 2003.
204. Wang, C.G. et al., In vitro bronchial responsiveness in two highly inbred rat strains. J Appl Physiol, 82, 1445, 1997.
205. Price, RJ. et al., Toxicity of 3-methylindole, 1-nitronaphthalene and paraquat in precision-cut rat lung slices. Arch Toxicol, 69, 405, 1995.
206. Placke, M.E. and Fisher, G.L., Adult peripheral lung organ culture — a model for respiratory tract toxicology. Toxicol Appl Pharmacol, 90, 284, 1987.
207. Vallan, C. et al., Release of a mitogenic factor by adult rat lung slices in culture. Exp Lung Res, 21, 469, 1995.
208. Kurosawa, H. et al., Mucociliary function in the mouse measured in explanted lung tissue. J Appl Physiol, 79, 41, 1995.
209. Fisher, R.L. et al., The use of human lung slices in toxicology. Hum Exp Toxicol, 13, 466, 1994.
210. Dandurand, RJ. et al., Responsiveness of individual airways to methacholine in adult rat lung explants. J Appl Physiol, 75, 364, 1993.
211. Warren, J.S. and Barton, P.A., In vitro analysis of pulmonary inflammation using rat lung organ cultures. Exp Lung Res, 18, 55, 1992.
212. Siminski, J.T. et al., Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions. J Appl Physiol, 262, L105, 1992.
213. Kinnard, W.V. et al., Regulation of alveolar type II cell differentiation and proliferation in adult rat lung explants. Am J Respir Cell Mol Biol, 11, 416, 1994.
214. Martin, C. et al., Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices. Eur Respir J, 9, 2479, 1996.
215. Martin, C. et al., Differential effects of the mixed ET(A)/ET(B)-receptor antagonist bosentan on endothelin-induced bronchoconstriction, vasoconstriction and prostacyclin release. Naunyn Schmiedebergs Arch Pharmacol, 362, 128, 2000.
216. Martin, C. et al., Cytokine-induced bronchoconstriction in precision-cut lung slices is dependent upon cyclooxygenase-2 and thromboxane receptor activation. Am J Respir Cell Mol Biol, 24, 139, 2001.
217. Martin, C. et al., Effects of the thromboxane receptor agonist U46619 and endothelin-1 on large and small airways. Eur Respir J, 16, 316, 2000.
218. Wohlsen, A. et al., The early allergic response in small airways of human precision-cut lung slices. Eur Respir J, 21, 1024, 2003.
219. Wohlsen, A. et al., Immediate allergic response in small airways. Am J Respir Crit Care Med, 163, 1462, 2001.
220. Williams, P.P. and Gallagher, J.E., Preparation and long-term cultivation of porcine tracheal and lung organ cultures by alternate exposure to gaseous and liquid medium phases. In Vitro Cell Dev Biol, 14, 686, 1978.
221. Held, H.D. et al., Characterization of airway and vascular responses in murine lungs. Br J Pharmacol, 126, 1191, 1999.
222. Jackson, R.M. et al., Lung MK 351A uptake after hypoxia adaptation and subsequent hyperoxia exposure. Lung, 166, 209, 1988.
223. Block, E.R. and Fisher, A.B., Depression of serotonin clearance by rat lungs during oxygen exposure. J Appl Physiol, 42, 33, 1977.
224. Pessina, G.P. et al., Pulmonary catabolism of interleukin-6 evaluated by lung perfusion of normal and smoker rats. J Pharm Pharmacol, 48, 1063, 1996.
225. Pessina, G.P. et al., Pulmonary catabolism of interferon-gamma evaluated by lung perfusion of both normal and smoke-exposed rats. J Interferon Cytokine Res, 15, 225, 1995.
226. Archer, S.L. et al., Detection of activated O2 species in vitro and in rat lungs by chemiluminescence. J Appl Physiol, 67, 1912, 1989.
227. Archer, S.L. et al., Simultaneous measurement of O2 radicals and pulmonary vascular reactivity in rat lung. J Appl Physiol, 67, 1903, 1989.
228. Joad, J.P. et al., Effects of ozone and neutrophils on function and morphology of the isolated rat lung. Am Rev Respir Dis, 147, 1578, 1993.
229. Joad, J.P. et al., Effect of respiratory pattern on ozone injury to the airways of isolated rat lungs. Toxicol Appl Pharmacol, 169, 26, 2000.
230. Joad, J.P. et al., The local C-fiber contribution to ozone-induced effects on the isolated guinea pig lung. Toxicol Appl Pharmacol, 141, 561, 1996.
231. Joad, J.P. et al., Ozone effects on mechanics and arachidonic acid metabolite concentrations in isolated rat lungs. Environ Res, 66, 186, 1994.
232. Postlethwait, E.M. et al., Three-dimensional mapping of ozone-induced acute cytotoxicity in tracheobronchial airways of isolated perfused rat lung. Am J Respir Cell Mol Biol, 22, 191, 2000.
233. Postlethwait, E.M. et al., Determinants of inhaled ozone absorption in isolated rat lungs. Toxicol Appl Pharmacol, 125, 77, 1994.
234. Delaunois, A. et al., Interactions between cytochrome P-450 activities and ozone-induced modulatory effects on endothelial permeability in rabbit lungs: influence of gender. Inhal Toxicol, 11, 999, 1999.
235. Delaunois, A. et al., Comparison of ozone-induced effects on lung mechanics and hemodynamics in the rabbit. Toxicol Appl Pharmacol, 150, 58, 1998.
236. Pino, M.V. et al., Functional and morphologic changes caused by acute ozone exposure in the isolated and perfused rat lung. Am Rev Respir Dis, 145, 882, 1992.
237. Bassett, DJ. et al., Rat lung recovery from 3 days of continuous exposure to 0.75 ppm ozone. J Toxicol Environ Health, 25, 329, 1988.
238. Joad, J.P. et al., Perinatal exposure to aged and diluted sidestream cigarette smoke produces airway hyperresponsiveness in older rats. Toxicol Appl Pharmacol, 155, 253, 1999.
239. Moller, L. et al., Metabolism of the carcinogenic air pollutant 2-nitrofluorene in the isolated perfused rat lung and liver. Carcinogenesis, 8, 1847, 1987.
240. Tornquist, S. et al., Bioavailability of benzo(a)pyrene deposited in the lung. Correlation with dissolution from urban air particulates and covalently bound DNA adducts. Drug Metab Dispos, 16, 842, 1988.
241. Gotze, J.P. et al., Effects of induction and age-dependent enzyme expression on lung bioavailability, metabolism, and DNA binding of urban air particulate-absorbed benzo[a]pyrene, 2-nitrofluorene, and 3-amino-1,4-dimethyl-5H-pyridol-(4,3)-indole. Environ Health Perspect, 102(Suppl. 4), 147, 1994.
242. Morgan, D.D. et al., The pharmacokinetics of benzo[alpha]pyrene in the isolated perfused rabbit lung: the influence of benzo[alpha]pyrene, n-dodecane, particulate, or sulfur dioxide. Toxicology, 33, 275, 1984.
243. Warshawsky, D. et al., Influence of airborne particulate on the metabolism of benzo[a]pyrene in the isolated perfused lung. J Toxicol Environ Health, 11, 503, 1983.
244. Schoeny, R. and Warshawsky, D., Mutagenicity of benzo(a)pyrene metabolites generated on the isolated perfused lung following particulate exposure. Teratog Carcinog Mutagen, 3, 151, 1983.
245. Becker, P.M. et al., Effects of oxygen tension and glucose concentration on ischemic injury in ventilated ferret lungs. J Appl Physiol, 75, 1233, 1993.
246. Brower, R.G. et al., Locus of hypoxic vasoconstriction in isolated ferret lungs. J Appl Physiol, 63, 58, 1987.
247. Tucker, A. et al., Pulmonary vascular responsiveness in lungs isolated from altitude-exposed and altitude recovery sympathectomized rats. Fed Proc, 45, 1031, 1986.
248. Tucker, A. et al., Altered vascular responsiveness in isolated perfused lungs from aging rats. Exp Lung Res, 3, 29, 1982.
249. McMurtry, I.F. et al., Inhibition of hypoxic pulmonary vasoconstriction by calcium antagonists in isolated rat lungs. Circ Res, 38, 99, 1976.
250. McMurtry, I.F. et al., Blunted hypoxic vasoconstriction in lungs from short-term high-altitude rats. Am J Physiol, 238, H849, 1980.
251. Allen, C.B. and White, C.W., Glucose modulates cell death due to normobaric hyperoxia by maintaining cellular ATP. Am J Physiol, 274, L159, 1998.
252. Holian, A. et al., Asbestos- and silica-induced changes in human alveolar macrophage phenotype. Environ Health Perspect, 105(Suppl. 5), 1139, 1997.
253. Sarady, J.K. et al., Carbon monoxide modulates endotoxin-induced production of granulocyte macrophage colony-stimulating factor in macrophages. Am J Respir Cell Mol Biol, 27, 739, 2002.
254. Richter, A. et al., Autocrine ligands for the epidermal growth factor receptor mediate interleukin-8 release from bronchial epithelial cells in response to cigarette smoke. Am J Respir Cell Mol Biol, 27, 85, 2002.
255. Bayram, H. et al., The effect of diesel exhaust particles on cell function and release of inflammatory mediators from human bronchial epithelial cells in vitro. Am J Respir Cell Mol Biol, 18, 441, 1998.
256. Jaspers, I. et al., Ozone-induced IL-8 expression and transcription factor binding in respiratory epithelial cells. Am J Physiol, 272, L504, 1997.
257. Cromwell, O. et al., Expression and generation of interleukin-8, IL-6 and granulocyte-macrophage colony-stimulating factor by bronchial epithelial cells and enhancement by IL-1 beta and tumour necrosis factor-alpha. Immunology, 77, 330, 1992.
258. Beck, N.B. et al., Ozone can increase the expression of intercellular adhesion molecule-1 by human nasal epithelial cells. Inhal Toxicol, 6, 345, 1994.
259. Spech, R.W. et al., Surfactant protein A prevents silica-mediated toxicity to rat alveolar macrophages. Am J Physiol Lung Cell Mol Physiol, 278, L713, 2000.
260. Persinger, R.L. et al., Nitrogen dioxide induces death in lung epithelial cells in a density-dependent manner. Am J Respir Cell Mol Biol, 24, 583, 2001.
261. Oortgiesen, M. et al., Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons. Am J Physiol Lung Cell Mol Physiol, 278, L683, 2000.