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Volumn 276, Issue 5317, 1997, Pages 1425-1428

Geometric control of cell life and death

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; COLLAGEN TYPE 1; DNA; FIBRONECTIN; GROWTH FACTOR; INTEGRIN; PROTEIN; SCLEROPROTEIN; VITRONECTIN;

EID: 0030953763     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5317.1425     Document Type: Article
Times cited : (4323)

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    • Human pulmonary microvascular endothelial cells (Clonetics) were cultured in EGM medium (Clonetics) supplemented with 2% fetal calf serum, epidermal growth factor (10 ng/ml), and fibroblast growth factor (FGF) (5 ng/ml). Bovine adrenal capillary endothelial cells were cultured in serum-free, chemically defined medium supplemented with FGF (5 ng/ml) (4).
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    • 1 (1 βg/ml; BD15; Biosource), or a combination of the two (0.5 μ/ml each). Cells cultured on substrates with no mouse antibody or antibodies to intracellular proteins did not adhere under these conditions.
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    • This work was supported by grants from NIH (HL57669, CA55833, GM30367), the Defense Advanced Research Projects Agency, and the Office of Naval Research; postdoctoral fellowships from the American Cancer Society (M.M.) and the Swiss National Science Foundation (S.H.); and partial salary support (C.S.C.) from the Harvard-MIT Health Sciences Technology Program.


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