Segmental isotope labelling of an individual bromodomain of a tandem domain BRD4 using Sortase A

PLoS ONE

Volumn 11, Issue 4, 2016, Pages

  Williams, Felix P ^a   Milbradt, Alexander G ^a   Embrey, Kevin J ^a   Bobby, Romel ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BROMODOMAIN CONTAINING PROTEIN 4; ISOLEUCINE; LEUCINE; MEMBRANE PROTEIN; METHYL GROUP; SORTASE; SORTASE A; UNCLASSIFIED DRUG; VALINE; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; BRD4 PROTEIN, HUMAN; CYSTEINE PROTEINASE; NUCLEAR PROTEIN; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; BIOPHYSICS; BROMODOMAIN; CARBON NUCLEAR MAGNETIC RESONANCE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; COVALENT BOND; DEUTERON NUCLEAR MAGNETIC RESONANCE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; HYDROLYSIS; ISOTOPE LABELING; LIGAND BROMODOMAIN INTERACTION; MOLECULAR INTERACTION; MOLECULAR MECHANICS; NITROGEN NUCLEAR MAGNETIC RESONANCE; PH; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN TERTIARY STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; REACTION TIME; SEGMENTAL ISOTOPE LABELLING; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; CHEMISTRY; GENETICS; HUMAN; MOLECULAR MODEL; NUCLEAR MAGNETIC RESONANCE; PROCEDURES;

AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; BIOPHYSICAL PHENOMENA; CYSTEINE ENDOPEPTIDASES; HUMANS; ISOTOPE LABELING; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; NUCLEAR PROTEINS; PROTEIN DOMAINS; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

EID: 84966356411     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0154607     Document Type: Article

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