Should we evaluate and treat sperm DNA fragmentation?

Current Opinion in Obstetrics and Gynecology

Volumn 28, Issue 3, 2016, Pages 164-171

  Agarwal, Ashok ^a   Cho, Chak Lam ^b   Esteves, Sandro C ^c  

^a LERNER RESEARCH INSTITUTE   (United States)

^b KWONG WAH HOSPITAL   (Hong Kong)

^c ANDROFERT   (Brazil)

Author keywords

assisted reproductive technology; DNA integrity; infertility; sperm DNA fragmentation

Indexed keywords

ANTIOXIDANT; DNA;

CONGENITAL MALFORMATION; DNA DAMAGE; DNA FRAGMENTATION; FERTILIZATION IN VITRO; HUMAN; INTRACYTOPLASMIC SPERM INJECTION; INTRAUTERINE INSEMINATION; MALE; MALE INFERTILITY; PREGNANCY; PREGNANCY OUTCOME; PRIORITY JOURNAL; PROGENY; REVIEW; SPERM DNA FRAGMENTATION; SPERM QUALITY; SPONTANEOUS ABORTION; VARICOCELECTOMY; FEMALE; FERTILIZATION; GENETICS; IN VITRO FERTILIZATION; INSEMINATION; PATHOLOGY; PREGNANCY RATE; SPERMATOZOON;

DNA FRAGMENTATION; FEMALE; FERTILIZATION; FERTILIZATION IN VITRO; HUMANS; INFERTILITY, MALE; INSEMINATION; MALE; PREGNANCY; PREGNANCY OUTCOME; PREGNANCY RATE; SPERM INJECTIONS, INTRACYTOPLASMIC; SPERMATOZOA;

EID: 84964033432     PISSN: 1040872X     EISSN: 1473656X     Source Type: Journal    
DOI: 10.1097/GCO.0000000000000271     Document Type: Review

References (76)

1. Guzick DS, Overstreet JW, Factor-Litvak P, et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001; 345: 1388-1393
2. Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science 1980; 210: 1131-1133
3. Irvine DS, Twigg JP, Gordon EL, et al. DNA integrity in human spermatozoa: relationships with semen quality. J Androl 2000; 21: 33-44
4. Sakkas D, Urner F, Bizzaro D, et al. Sperm DNA damage and altered chromatin structure: effect on fertilization and embryo development. Hum Reprod 1998; 4 (Suppl 13): 11-19
5. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677
6. Huang CC, Lin DP, Tsao HM, et al. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 2005; 84: 130-140
7. Saleh RA, Agarwal A, Nelson DR, et al. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril 2002; 78: 313-318
8. Evgeni E, Lymberopoulos G, Gazouli M, Asimakopoulos B. Conventional semen parameters and DNA fragmentation in relation to fertility status in a Greek population. Eur J Obstet Gynecol Reprod Biol 2015; 188: 17-23
9. Twigg JP, Irvine DS, Aitken RJ. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Hum Reprod 1998; 13: 1864-1871
10. Gil-Guzman E, Ollero M, Lopez MC, et al. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001; 16: 1922-1930
11. Ollero M, Gil-Guzman E, Lopez MC, et al. Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod 2001; 16: 1912-1921
12. Aktan G, Dogru-Abbasoglu S, Kucukgergin C, et al. Mystery of idiopathic male infertility: is oxidative stress an actual risk?. Fertil Steril 2013; 99: 1211-1215
13. Wright C, Milne S, Leeson H. Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility. Reprod Biomed Online 2014; 28: 684-703
14. Dalzell LH, McVicar CM, McClure N, et al. Effects of short and long incubations on DNA fragmentation of testicular sperm. Fertil Steril 2004; 82: 1443-1445
15. Esteves SC, Agarwal A. Novel concepts in male infertility. Int Braz J Urol 2011; 37: 5-15
16. Zini A. Are sperm chromatin and DNA defects relevant in the clinic?. Syst Biol Reprod Med 2011; 57: 78-85
17. Spano M, Bonde JP, Hjollund HI, et al. The Danish First Pregnancy Planner Study Team. Sperm chromatin damage impairs human fertility. Fertil Steril 2000; 73: 43-50
18. Bungum M, Humaidan P, Axmon A, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod 2007; 22: 174-179
19. Duran EH, Morshedi M, Taylor S, Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 2002; 17: 3122-3128
20. Muriel L, Meseguer M, Fernandez JL, et al. Value of the sperm chromatin dispersion test in predicting pregnancy outcome in intrauterine insemination: a blind prospective study. Hum Reprod 2006; 21: 738-744
21. Zhang Z, Zhu L, Jiang H, et al. Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-Analysis. J Assist Reprod Genet 2015; 32: 17-26
22. Collins JA, Barnhart KT, Schlegel PN. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization?. Fertil Steril 2008; 89: 823-831
23. Anifandis G, Bounartzi T, Messini CI, et al. Sperm DNA fragmentation measured by Halosperm does not impact on embryo quality and ongoing pregnancy rates in IVF/ICSI treatments. Andrologia 2015; 47: 295-302
24. Wdowiak A, Bakalczuk S, Bakalczuk G. The effect of sperm DNA fragmentation on the dynamics of the embryonic development in intracytoplasmatic sperm injection. Reprod Biol 2015; 15: 94-100
25. Osman A, Alsomait H, Seshadri S, et al. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-Analysis. Reprod Biomed Online 2015; 30: 120-127
26. Zini A, Boman JM, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-Analysis. Hum Reprod 2008; 23: 2663-2668
27. Zhao J, Zhang Q, Wang Y, Li Y. Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization/intracytoplasmic sperm injection: a systematic review and metaanalysis. Fertil Steril 2014; 102: 998-1005
28. Robinson L, Gallos ID, Conner SJ, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and metaanalysis. Hum Reprod 2012; 27: 2908-2917
29. Khadem N, Poorhoseyni A, Jalali M, et al. Sperm DNA fragmentation in couples with unexplained recurrent spontaneous abortions. Andrologia 2014; 46: 126-130
30. The Practice Committee of the American Society for Reproductive Medicine. The clinical utility of sperm DNA integrity testing: a guideline. Fertil Steril 2013; 99: 673-677
31. Practice Committee of the American Society for Reproductive Medicine. Diagnostic evaluation of the infertile male: a committee opinion. Fertil Steril 2015; 103: e18-e25
32. Chohan KR, Griffin JT, Lafromboise M, et al. Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J Androl 2006; 27: 53-59
33. Esteves SC, Sharma RK, Gosalvez J, Agarwal A. A translational medicine appraisal of specialized andrology testing in unexplained male infertility. Int Urol Nephrol 2014; 46: 1037-1052
34. Drobnis EZ, Johnson MH. Are we ready to incorporate sperm DNA fragmentation testing into our male infertility work-up?. A plea for more robust studies. Reprod Biomed Online 2015; 30: 111-112
35. Jin J, Pan C, Fei Q, et al. Effect of sperm DNA fragmentation on the clinical outcomes for in vitro fertilization and intracytoplasmic sperm injection in women with different ovarian reserves. Fertil Steril 2015; 103: 910-916
36. Menezo Y Jr, Russo G, Tosti E, et al. Expression profile of genes coding for DNA repair in human oocytes using pangenomic microarrays, with a special focus on ROS linked decays. J Assist Reprod Genet 2007; 24: 513-520
37. Stahl PJ, Cogan C, Mehta A, et al. Concordance among sperm deoxyribonucleic acid integrity assays and semen parameters. Fertil Steril 2015; 104: 56-61
38. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345
39. Esteves SC, Gosalvez J, Lopez-Fernandez C, et al. Diagnostic accuracy of sperm DNA degradation index (DDSi) as a potential noninvasive biomarker to identify men with varicocele-Associated infertility. Int Urol Nephrol 2015; 47: 1471-1477
40. Feijo CM, Esteves SC. Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility. Fertil Steril 2014; 101: 58-63
41. Tesarik J, Greco E, Mendoza C. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation. Hum Reprod 2004; 19: 611-615
42. Seli E, Gardner DK, Schoolcraft WB, et al. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril 2004; 82: 378-383
43. Meseguer M, Martinez-Conejero JA, O'Connor JE, et al. The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility prognostic factor. Fertil Steril 2008; 89: 1191-1199
44. Simon L, Murphy K, Shamsi MB, et al. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod 2014; 29: 2402-2412
45. Simon L, Liu L, Murphy K, et al. Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment. Hum Reprod 2014; 29: 904-917
46. Bonduelle M, Van Assche E, Joris H, et al. Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters. Hum Reprod 2002; 17: 2600-2614
47. Enciso M, Alfarawati S, Wells D. Increased numbers of DNA-damaged spermatozoa in samples presenting an elevated rate of numerical chromosome abnormalities. Hum Reprod 2013; 28: 1707-1715
48. Ramasamy R, Scovell JM, Kovac JR, et al. Fluorescence in situ hybridization detects increased sperm aneuploidy in men with recurrent pregnancy loss. Fertil Steril 2015; 103: 906-909
49. Fernandez-Gonzalez R, Moreira PN, Perez-Crespo M, et al. Long-Term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring. Biol Reprod 2008; 78: 761-772
50. Fraga CG, Motchnik PA, Wyrobek AJ, et al. Smoking and low antioxidant levels increase oxidative damage to DNA. Mutat Res 1996; 351: 199-203
51. Ji BT, Shu XO, Linet MS, et al. Paternal cigarette smoking and the risk of childhood cancer among offspring of nonsmoking mothers. J Natl Cancer Inst 1997; 89: 238-244
52. Lee KM, Ward MH, Han S, et al. Paternal smoking, genetic polymorphisms in CYP1A1 and childhood leukemia risk. Leukemia Res 2009; 33: 250-258
53. Schmid TE, Eskenazi B, Baumgartner A, et al. The effects of male age on sperm DNA damage in healthy nonsmokers. Hum Reprod 2007; 22: 180-187
54. Crow JF. The origins, patterns and implications of human spontaneous mutation. Nat Rev Genet 2000; 1: 40-47
55. Sipos A, Rasmussen F, Harrison G, et al. Paternal age and schizophrenia: a population based cohort study. BMJ 2004; 329: 1070-1073
56. McIntosh CG, Olshan AF, Baird PA. Paternal age and the risk of birth defects in offspring. Epidemiology 1995; 6: 282-288
57. Lewis SE, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 2005; 322: 33-41
58. Chavarro JE, Toth TL, Sadio SM, Hauser R. Soy food and isoflavone intake in relation to semen quality parameters among men from an infertility clinic. Hum Reprod 2008; 23: 2584-2590
59. Greco E, Romano S, Iacobelli M, et al. ICSI in cases of sperm DNA damage: beneficial effect of oral antioxidant treatment. Hum Reprod 2005; 20: 2590-2594
60. Tremellen K, Miari G, Froiland D, Thompson J. A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment. Aust N Z J Obstet Gynaecol 2007; 47: 216-221
61. Showell MG, Mackenzie-Proctor R, Brown J, et al. Antioxidants for male subfertility. Cochrane Database Syst Rev 2014; 12: CD007411
62. Cho CL, Esteves SC, Agarwal A. Novel insights into the pathophysiology of varicocele and its association with reactive oxygen species and sperm DNA fragmentation. Asian J Androl 2016; 18: 186-193
63. Zini A, Dohle G. Are varicoceles associated with increased deoxyribonucleic acid fragmentation?. Fertil Steril 2011; 96: 1283-1287
64. Wang YJ, Zhang RQ, Lin YJ, et al. Relationship between varicocele and sperm DNA damage and the effect of varicocele repair: a meta-Analysis. Reprod Biomed Online 2012; 25: 307-314
65. Sakkas D, Manicardi GC, Tomlinson M, et al. The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod 2000; 15: 1112-1116
66. Sa R, Cunha M, Rocha E, et al. Sperm DNA fragmentation is related to sperm morphological staining patterns. Reprod Biomed Online 2015; 31: 506-515
67. Fleming SD, Ilad RS, Griffin AM, et al. Prospective controlled trial on an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation. Hum Reprod 2008; 23: 2646-2651
68. Jakab A, Sakkas D, Delpiano E, et al. Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil Steril 2005; 84: 1665-1673
69. Degheidy T, Abdelfattah H, Seif A, et al. Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques. Andrologia 2015; 47: 892-896
70. Bucar S, Goncalves A, Rocha E, et al. DNA fragmentation in human sperm after magnetic-Activated cell sorting. J Assist Reprod Genet 2015; 32: 147-154
71. Berkovitz A, Eltes F, Yaari S, et al. The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod 2005; 20: 185-190
72. Gandini L, Lombardo F, Paoli D, et al. Full-Term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Hum Reprod 2004; 19: 1409-1417
73. Celik-Ozenci C, Jakab A, Kovacs T, et al. Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. Hum Reprod 2004; 19: 2052-2059
74. Greco E, Scarselli F, Iacobelli M, et al. Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod 2005; 20: 226-230
75. Esteves SC, Sanchez-Martin F, Sanchez-Martin P, et al. Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. Fertil Steril 2015; 104: 1398-1405
76. Gosalvez J, Lopez-Fernandez C, Fernandez JL, et al. Unpacking the mysteries of sperm DNA fragmentation: ten frequently asked questions. J Reprod Biotechnol Fertil 2015; 4: 1-16