DNA integrity in human spermatozoa: Relationships with semen quality

Journal of Andrology

Volumn 21, Issue 1, 2000, Pages 33-44

  Irvine, D Stewart ^a   Twigg, Jeremy P ^a   Gordon, Emma L ^a   Fulton, Norma ^a   Milne, Philip A ^a   Aitken, R John ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

CASA; Comet assay; ICSI; In situ nick translation; Infertility; Spermatogenesis

Indexed keywords

REACTIVE OXYGEN METABOLITE;

ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA NICK TRANSLATION; HUMAN; HUMAN CELL; MALE INFERTILITY; PRIORITY JOURNAL; SEMEN ABNORMALITY; SEMEN ANALYSIS; SPERMATOZOON;

DNA; DNA DAMAGE; FORECASTING; GENETIC TECHNIQUES; HUMANS; MALE; REACTIVE OXYGEN SPECIES; SEMEN; SPERM COUNT; SPERMATOZOA;

EID: 0033978572     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (58)

1. Agarwal A, Ikemoto I, Loughlin KR. Effect of sperm washing on levels of reactive oxygen species in semen. Arch Androl 1994;33:157-162.
2. Aitken RJ, Buckingham DW, Brindle J, Gomez E, Baker HWG, Irvine DS. Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma. Hum Reprod 1995;10:2061-2071.
3. Aitken RJ, Buckingham DW, West KM. Reactive oxygen species and human spermatozoa - analysis of the cellular mechanisms involved in luminol-dependent and lucigenin-dependent chemiluminescence. J Cell Physiol 1992;151:466-477.
4. Aitken RJ, Buckingham DW, West K, Brindle J. On the use of paramagnetic heads and ferrofluids to assess and eliminate the leukocytic contribution to oxygen radical generation by human sperm suspensions. Am J Reprod Immunol 1996;35:541-551.
5. Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipid-peroxidation, and human-sperm function. Biol Reprod 1989a;41: 183-197.
6. Aitken RJ, Clarkson JS, Hargreave TB, Irvine DS, Wu FCW. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. J Androl 1989b;10:214-220.
7. Aitken RJ, Irvine DS, Wu FC. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am J Obstet Gynecol 1991;164:542-551.
8. Aitken RJ, West K, Buckingham D. Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function. J Androl 1994;15:343-352.
9. Altman SA, Zastawny TH, RandersEichhorn L, Cacciuttolo MA, Akman SA, Dizdaroglu M, Rao G. Formation of DNA-protein cross-links in cultured mammalian cells upon treatment with iron ions. Free Rad Biol Med 1995;19:897-902.
10. Baccetti B, Collodel G, Piomboni P. Apoptosis in human ejaculated sperm cells. J Submicrosc Cytol Pathol 1996;28:587-596.
11. Bar-Hava I, Ashkenazi J, Shelef M, Schwartz A, Brengauz M, Feldberg D, Orvieto R, Ben-Rafael Z. Morphology and clinical outcomes of embryos after in vitro fertilization are superior to those after intracytoplasmic sperm injection. Fertil Steril 1997;68:653-657.
12. Bianchi PG, Manicardi GC, Bizzaro D, Bianchi U, Sakkas D. Effect of deoxyribonucleic acid protamination on fluorochrome staining and in situ nick-translation of murine and human mature spermatozoa. Biol Reprod 1993;49:1083-1088.
13. Biggers J, Whitten W, Whittingham D. The Culture of Mouse Embryos in Vitro. San Francisco: Freeman; 1971.
14. Bonduelle M, Legein J, Buysse A, Van Assche E, Wisanto A, Devroey P, Van Steirteghem AC, Liebaers I. Prospective follow-up study of 423 children born after intracytoplasmic sperm injection. Hum Reprod 1996a;11:1558-1564.
15. Bonduelle M, Wilikens A, Buysse A, Van Assche E, Wisanto A, Devroey P, Van Steirteghem AC, Liebaers I. Prospective follow-up study of 877 children born after intracytoplasmic sperm injection (ICSI), with ejaculated epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI. Hum Reprod (suppl 4) 1996b;11:131-155.
16. Cummins JM, Jequier AM. Concerns and recommendations for intracytoplasmic sperm injection (ICSI) treatment. Hum Reprod 1995;10: 138-143.
17. De Jong ASH, Van Kessel-Van Vark M, Raap AK. Sensitivity of various visualisation methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. Histochem J 1985;17:1119-1130.
18. Dizdaroglu M. Oxidative damage to DNA in mammalian chromatin. Mutat Res 1992;275:331-342.
19. Foresta C, Rossato M, Garolla A, Ferlin A. Male infertility and ICSI: are there limits? Hum Reprod 1996;11:2347-2348.
20. Fraga CG, Motchnik PA, Wyrobek AJ, Rempel DM, Ames BN. Smoking and low antioxidant levels increase oxidative damage to sperm DNA. Mutat Res 1996;351:199-203.
21. Glazener CM, Kelly NJ, Weir MJ, David JS, Cornes JS, Hull MG. The diagnosis of male infertility - prospective time-specific study of conception rates related to seminal analysis and post-coital sperm-mucus penetration and survival in otherwise unexplained infertility. Hum Reprod 1987;2:665-671.
22. Gomez E, Buckingham DW, Brindle J, Lanzafame F, Irvine DS, Aitken RJ. Development Of an image-analysis system to monitor the retention of residual cytoplasm by human spermatozoa - correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function. J Androl 1996;17:276-287.
23. Hughes CM, Lewis SEM, McKelvey-Martin VJ, Thompson W. A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol Reprod Dev 1996;2:613-619.
24. Hull MGR. The causes of infertility and relative effectiveness of treatment. In: Templeton AA, Drife JO, eds. Infertility. Berlin: Springer-Verlag; 1992:33-57.
25. Huszar G, Patrizio P, Vigue L, Willets M, Wilker C, Adhoot D, Johnson L. Cytoplasmic extrusion and the switch from creatine kinase B to M isoform are completed by the commencement of epididymal transport in human and stallion spermatozoa. J Androl 1998;19:11-20.
26. Huszar G, Vigue L. Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 1994;15:71-77.
27. Irvine DS, Templeton AA. Donor insemination. In: Hargreave, TB, ed. Male Infertility. Edinburgh, United Kingdom: Churchill Livingstone; 1994:427-451.
28. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992;57:409-416.
29. Ji BT, Shu XO, Linet MS, Zheng W, Wacholder S, Gao YT, Ying DM, Jin F. Paternal cigarette smoking and the risk of childhood cancer among offspring of nonsmoking mothers. J Natl Cancer Inst 1997; 89:238-244.
30. Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997;68:519-524.
31. Kurinczuk J, Bower C. Birth defects in infants conceived by intracytoplasmic sperm injection: an alternative interpretation. Br Med J 1997; 315:1260-1263.
32. Lalwani S, Sayme N, Vigue L, Corrales M, Huszar G. Biochemical markers of early and late spermatogenesis: relationship between the lactate dehydrogenase-X and creatine kinase-M isoform concentrations in human spermatozoa. Mol Reprod Dev 1996;43:495-502.
33. Leroy T, Van Hummelen P, Anard D, Castelain P, KirschVolders M, Lauwerys R, Lison D. Evaluation of three methods for the detection of DNA single-strand breaks in human lymphocytes: alkaline elution, nick translation, and single-cell gel electrophoresis. J Toxicol Env Health 1996;47:409-422.
34. Lloyd DR, Phillips DH, Carmichael PL. Generation of putative intrastrand cross-links and strand breaks in DNA by transition metal ion-mediated oxygen radical attack. Chem Res Toxicol 1997;10:393-400.
35. Manicardi G, Bianchi P, Pantano S, Azzoni P, Bizzaro D, Bianchi U, Sakkas D. Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromonycin A3 accessibility. Biol Reprod 1995;52:864-867.
36. Martins EAL, Meneghini R. Cellular DNA damage by hydrogen peroxide is attenuated by hypotonicity. Biochem J 1994;299:137-140.
37. McKelvey-Martin VJ, Green MHL, Schmezer P, Pool-Zobel BL, De Meo MP, Collins A. The single cell gel electrophoresis assay (comet assay): a European review. Mutat Res 1993;288:47-63.
38. Palomba L, Brambilla L, Brandi G, Sestili P, Cattabeni F, Cantoni O. Low levels of hydrogen peroxide and L-histidine induce DNA double-strand breakage and apoptosis. Eur J Pharmacol 1996;318:167-173.
39. Sakkas D, Manicardi G, Bianci P, Bizzaro D, Bianchi U. Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilising mouse spermatozoa. Biol Reprod 1995;52:1149-1155.
40. Sakkas D, Urner F, Bianchi PG, Bizzaro D, Wagner I, Jaquenoud N, Manicardi G, Campana A. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 1996;11:837-843.
41. Schmidt L, Münster K, Helm P. Infertility and the seeking of infertility treatment in a representative population. Brit J Obstet Gynaecol 1995; 102:978-984.
42. Shen HM, Chia SE, Ni ZY, New AL, Lee BL, Ong CN. Detection of oxidative DNA damage in human sperm and the association with cigarette smoking. Reprod Toxicol 1997;11:675-680.
43. Singh NP. Microgel electrophoresis of DNA. In: G. P. Pfeifer, ed. Technologies for Detection of DNA Damage and Mutations. New York: Plenum Press; 1996:3-24.
44. Singh NP, Danner DB, Tice RR, McCoy MC, Collins GD, Schneider EL. Abundant alkali labile sites in DNA of human and mouse sperm. Exp Cell Res 1989;184:461-470.
45. Smith R, Vantman D, Ponce J, Escobar J, Lissi E. Total antioxidant capacity of human seminal plasma. Hum Reprod 1996;11:1655-1660.
46. Sorahan T, Lancashire RJ, Hulten MA, Peck I, Stewart AM. Childhood cancer and parental use of tobacco: deaths from 1953 to 1955. Br J Cancer 1997;75:134-138.
47. Sukcharoen N, Keith J, Irvine DS, Aitken RJ. Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function-tests - the effect of the delay between testing and IVF. Hum Reprod 1996;11:1030-1034.
48. Sun JG, Jurisicova A, Casper RF. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod 1997;56:602-607.
49. Templeton AA. The epidemiology of infertility. In: Templeton AA, Drife JO, eds. Infertility. Berlin: Springer-Verlag; 1992:23-34.
50. Thonneau P, Marchand S, Tallec A, Ferial M-L, Ducot B, Lansac J, Lopes P, Tabaste J-M, Spira A. Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988-1989). Hum Reprod 1991;6:811-816.
51. Twigg J, Irvine D, Aitken R. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at ICSI. Hum Reprod 1997;13:1864-1871.
52. Twigg J, Irvine DS, Houston P, Fulton N, Michael L, Aitken RJ. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma. Mol Hum Reprod 1998;4:439-445.
53. Van Steirteghem A, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde M, Van Assche E, Devroey P. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993a;8:1055-1060.
54. Van Steirteghem A, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, Wisanto A, Devroey P. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993b;8:1061-1066.
55. Wisanto A, Bonduelle M, Camus M, Tournaye H, Magnus M, Liebaers I, Van Steirteghem A, Devroey P. Obstetric outcome of 904 pregnancies after intracytoplasmic sperm injection. Hum Reprod 1996;11: 121-129.
56. Wood G, Warnke R. Suppresion of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981;29:1196-1204.
57. World Health Organization (1992). WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. 3rd ed. Cambridge, United Kingdom: Cambridge University Press; 1987.
58. Zastawny TH, Altman SA, Randers-Eichhorn L, Madurawe R. Lumpkin JA, Didzaroglu M, Rao G. DNA base modifications and membrane damage in cultured mammalian cells treated with iron ions. Free Rad Biol Med 1995;18:1013-1022.