Characterization of subsets of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility

Human Reproduction

Volumn 16, Issue 9, 2001, Pages 1912-1921

  Ollero, Mario ^a   Gil Guzman, Enrique ^b   Lopez, Mari C ^a   Sharma, Rakesh K ^b   Agarwal, Ashok ^b   Larson, Kjersten ^c   Evenson, Donald ^c   Thomas Jr, Anthony J ^b   Alvarez, Juan G ^a,d  

^a HARVARD MEDICAL SCHOOL   (United States)

^b LERNER RESEARCH INSTITUTE   (United States)

^c SOUTH DAKOTA STATE UNIVERSITY   (United States)

^d BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

Chromatin; Docosahexaenoic acid; ROS; Sperm preparation; Spermatozoa

Indexed keywords

DNA; DOCOSAHEXAENOIC ACID; LIPID; PHOSPHOLIPID; REACTIVE OXYGEN METABOLITE;

ARTICLE; ASSAY; CHROMATIN; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION FUNCTION; CYTOPLASM; DENSITY; DNA STRUCTURE; EPIDIDYMIS; HUMAN; LIPID COMPOSITION; MALE; MALE INFERTILITY; MEMBRANE DAMAGE; MORPHOLOGY; PATHOGENESIS; SEMINIFEROUS TUBULE; SPERM; SPERMATOGENESIS; SPERMATOZOON; SPERMATOZOON MATURATION;

EID: 0034814823     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/16.9.1912     Document Type: Article

References (70)

1. A free radical theory of male infertility
2. Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk
3. Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation and the presence of leukocytes and precursor germ cells in human sperm suspensions
4. On the use of paramagnetic beads and ferrofluids to assess and eliminate the leukocytic contribution to oxygen radical generation by human sperm suspensions
5. Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitor diphenylene iodonium and quinacrine
6. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa
7. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
8. Separation of acidic and neutral lipids by aminopropyl-bonded silica gel column chromatography
9. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa
10. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity
11. The epididymis and sperm maturation: A perspective
12. Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single-cell electrophoresis
13. Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa
14. Gonadal cell apoptosis: Hormone-regulated cell demise
15. Repair of the ultraviolet-irradiated male genome in fertilized mouse eggs
16. Partial obstruction, not antisperm antibodies, causing infertility after vasovasostomy
17. Comparative evaluation of two density gradient preparations for sperm separation for medically assisted conception
18. Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry
19. Reactive oxygen species (ROS) and reproduction
20. Spermatozoon maturation in vertebrates with internal fertilization
21. Cryopreservation of human spermatozoa with pentoxifylline improves the post-thaw agonist-induced acrosome reaction
22. Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects
23. Rapid analysis of normal and abnormal cell types in human semen and testis biopsies by flow cytometry
24. Sperm chromatin structure assay: DNA denaturability
25. Relation of mammalian sperm heterogeneity to fertility
26. Flow cytometric analysis of mouse spermatogenic function following exposure to ethylnitrosourea
27. Long term effects of triethylmelamine exposure on mouse testis cells and sperm chromatin structure assayed by flow cytometry
28. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay
29. Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility
30. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
31. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm
32. Smoking and low antioxidant levels increase oxidative damage to sperm DNA
33. Repair of human sperm chromosome aberrations in the hamster egg
34. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation
35. Presence of DNA strand breaks and increased sensitivity of DNA in situ denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells
36. Epididymal sperm maturation proteins
37. Varicocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity
38. Population study of causes, treatment and outcome of infertility
39. Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa
40. Cytoplasmic extrusion and the switch from creatine kinase B to M isoform are completed by the commencement of epididymal transport in human and stallion spermatozoa
41. Membrane remodelling during sperm maturation in the epididymis
42. Sperm morphologic features as a prognosis factor of in vitro fertilization
43. Density gradient centrifugation and glass wool filtration of semen remove sperm with damaged chromatin
44. Relationship between assisted reproductive techniques (ART) outcome and status of chromatin integrity as measured by the Sperm Chromatin Structure Assay (SCSA)
45. Reactive oxygen species: Potential cause for DNA fragmentation in human spermatozoa
46. Proportion of cystic fibrosis gene mutations not detected by routine testing in men with obstructive azoospermia
47. Chromosomal analysis in mouse eggs fertilized in vitro with sperm exposed to ultraviolet light and methyl and ethyl methansulfonate
48. Localization of DNase I-hypersensitive regions during rat spermatogenesis: Stage-dependent patterns and unique sensitivity of elongating spermatids
49. Nicking of rat spermatid and spermatozoa DNA: Possible involvement of DNA tropoisomerase II
50. Chromatin structure-function alterations during mammalian spermatogenesis: DNA nicking and repair in elongating spermatids
51. Comparison of a discontinuous Percoll gradient method versus a swim-up method: Effects on sperm morphology and other semen parameters
52. Methods of sperm preparation for assisted reproduction
53. Sperm quality may adversely affect the chromosome constitution of embryos that result from intracytoplasmic sperm injection
54. Variation in docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: Implications for sperm lipoperoxidative damage
55. Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury
56. Effects of cancer on sperm quality after cryopreservation: A 12-year experience
57. Influence of sperm parameters on embryo quality
58. Fas regulates germ cell apoptosis in the human testis in vitro
59. The phospholipid-bound fatty acids and aldehydes of mammalian spermatozoa
60. Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the Terminal Deoxynucleotidyl Transferase Assay
61. Bull sperm head morphometry related to abnormal chromatin structure and fertility
62. Origin of DNA damage in ejaculated human spermatozoa
63. Role of reactive oxygen species in male infertility
64. Positive myeloperoxidase staining (Endtz test) as an indicator of excessive reactive oxygen species formation in semen
65. How is male infertility defined?
66. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: Lipid peroxidation, DNA fragmentation and effectiveness of antioxidants
67. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection
68. Generation of reactive oxygen species by leukocytes and sperm following exposure to urogenital tract infection
69. The fatty acid composition of phospholipids of spermatozoa from infertile patients