GPR18 undergoes a high degree of constitutive trafficking but is unresponsive to N-Arachidonoyl Glycine

PeerJ

Volumn 2016, Issue 3, 2016, Pages

  Finlay, David B ^a   Joseph, Wayne R ^b   Grimsey, Natasha L ^a   Glass, Michelle ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Cannabinoid; GPCR; GPR18; N arachidonoyl glycine; NAGly; Orphan receptor

Indexed keywords

ALANINE; ANANDAMIDE; ASPARAGINE; CANNABINOID 1 RECEPTOR; CYCLIC AMP; ENDOCANNABINOID; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR 18; LIGAND; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; N ARACHIDONOYL GLYCINE; UNCLASSIFIED DRUG;

APOPTOSIS; AREA UNDER THE CURVE; ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL CULTURE; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; GENE ACTIVATION; GENE EXPRESSION; GLIOBLASTOMA; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; MOLECULAR CLONING; POINT MUTATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TRANSIENT TRANSFECTION;

EID: 84963966363     PISSN: None     EISSN: 21678359     Source Type: Journal    
DOI: 10.7717/peerj.1835     Document Type: Article

References (39)

1. Atwood BK, Lopez J, Wager-Miller J, Mackie K, Straiker A. 2011. Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis. BMC Genomics 12:14 DOI 10.1186/1471-2164-12-14.
2. Belin D, Bost S, Vassalli JD, Strub K. 1996. A two-step recognition of signal sequences determines the translocation efficiency of proteins. EMBO Journal 15:468-478.
3. Bradshaw HB, Lee SH, McHugh D. 2009. Orphan endogenous lipids and orphan GPCRs: a good match. Prostaglandins & Other Lipid Mediators 89:131-134 DOI 10.1016/j.prostaglandins.2009.04.006.
4. Caldwell MD, Hu SS, Viswanathan S, Bradshaw H, Kelly ME, Straiker A. 2013. A GPR18-based signalling system regulates IOP in murine eye. British Journal of Pharmacology 169:834-843 DOI 10.1111/bph.12136.
5. Cawston EE, Redmond WJ, Breen CM, Grimsey NL, Connor M, Glass M. 2013. Realtime characterization of cannabinoid receptor 1 (CB1) allosteric modulators reveals novel mechanism of action. British Journal of Pharmacology 170:893-907 DOI 10.1111/bph.12329.
6. Drake MT, Shenoy SK, Lefkowitz RJ. 2006. Trafficking of G protein-coupled receptors. Circulation Research 99:570-582 DOI 10.1161/01.RES.0000242563.47507.ce.
7. Gantz I, Muraoka A, Yang YK, Samuelson LC, Zimmerman EM, Cook H, Yamada T. 1997. Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis. Genomics 42:462-466 DOI 10.1006/geno.1997.4752.
8. Gibbons H, Sato TA, Dragunow M. 2003. Hypothermia suppresses inducible nitric oxide synthase and stimulates cyclooxygenase-2 in lipopolysaccharide stimulated BV-2 cells. Brain Research. Molecular Brain Research 110:63-75 DOI 10.1016/S0169-328X(02)00585-5.
9. Grimsey NL, Graham ES, Dragunow M, Glass M. 2010. Cannabinoid Receptor 1 trafficking and the role of the intracellular pool: implications for therapeutics. Biochemical Pharmacology 80:1050-1062 DOI 10.1016/j.bcp.2010.06.007.
10. Grimsey NL, Narayan PJ, Dragunow M, Glass M. 2008. A novel high-throughput assay for the quantitative assessment of receptor trafficking. Clinical and Experimental Pharmacology and Physiology 35:1377-1382 DOI 10.1111/j.1440-1681.2008.04991.x.
11. Gudermann T. 2001. Multiple pathways of ERK activation by G protein-coupled receptors. Novartis Foundation Symposium 239:68-79 discussion 80-64, 150-159 DOI 10.1002/0470846674.ch7.
12. Huang SM, Bisogno T, Petros TJ, Chang SY, Zavitsanos PA, Zipkin RE, Sivakumar R, Coop A, Maeda DY, De Petrocellis L, Burstein S, Di Marzo V, Walker JM. 2001. Identification of a new class of molecules, the arachidonyl amino acids, and characterization of one member that inhibits pain. Journal of Biological Chemistry 276:42639-42644 DOI 10.1074/jbc. M107351200.
13. Ivic L, Zhang C, Zhang X, Yoon SO, Firestein S. 2002. Intracellular trafficking of a tagged and functional mammalian olfactory receptor. Journal of Neurobiology 50:56-68 DOI 10.1002/neu.10016.
14. Kohno M, Hasegawa H, Inoue A, Muraoka M, Miyazaki T, Oka K, Yasukawa M. 2006. Identification of N-arachidonylglycine as the endogenous ligand for orphan Gprotein-coupled receptor GPR18. Biochemical and Biophysical Research Communi-cations 347:827-832 DOI 10.1016/j.bbrc.2006.06.175.
15. Komiya Y, Habas R. 2008. Wnt signal transduction pathways. Organogenesis 4:68-75 DOI 10.4161/org.4.2.5851.
16. Kurzchalia TV, Wiedmann M, Girshovich AS, Bochkareva ES, Bielka H, Rapoport TA. 1986. The signal sequence of nascent preprolactin interacts with the 54K polypeptide of the signal recognition particle. Nature 320:634-636 DOI 10.1038/320634a0.
17. Leterrier C, Bonnard D, Carrel D, Rossier J, Lenkei Z. 2004. Constitutive endocytic cycle of the CB1 cannabinoid receptor. Journal of Biological Chemistry 279:36013-36021 DOI 10.1074/jbc. M403990200.
18. Lew M. 2007. Good statistical practice in pharmacology. Problem 2. British Journal of Pharmacology 152:299-303 DOI 10.1038/sj.bjp.0707372.
19. Lu VB, Puhl 3rd HL, Ikeda SR. 2013. N-Arachidonyl glycine does not activate G proteincoupled receptor 18 signaling via canonical pathways. Molecular Pharmacology 83:267-282 DOI 10.1124/mol.112.081182.
20. Mantovani A, Bonecchi R, Locati M. 2006. Tuning inflammation and immunity by chemokine sequestration: decoys and more. Nature Reviews Immunology 6:907-918 DOI 10.1038/nri1964.
21. Mantovani A, Locati M, Vecchi A, Sozzani S, Allavena P. 2001. Decoy receptors: a strategy to regulate inflammatory cytokines and chemokines. Trends in Immunology 22:328-336 DOI 10.1016/S1471-4906(01)01941-X.
22. Mason JN, Kozell LB, Neve KA. 2002. Regulation of dopamine D(1) receptor trafficking by protein kinase A-dependent phosphorylation. Molecular Pharmacology 61:806-816 DOI 10.1124/mol.61.4.806.
23. McDonald NA, Henstridge CM, Connolly CN, Irving AJ. 2007. An essential role for constitutive endocytosis, but not activity, in the axonal targeting of the CB1 cannabinoid receptor. Molecular Pharmacology 71:976-984 DOI 10.1124/mol.106.029348.
24. McHugh D, Hu SS, Rimmerman N, Juknat A, Vogel Z, Walker JM, Bradshaw HB. 2010. N-arachidonoyl glycine, an abundant endogenous lipid, potently drives directed cellular migration through GPR18, the putative abnormal cannabidiol receptor. BMC Neuroscience 11:44 DOI 10.1186/1471-2202-11-44.
25. McHugh D, Page J, Dunn E, Bradshaw HB. 2012a. Delta(9)-Tetrahydrocannabinol and N-arachidonyl glycine are full agonists at GPR18 receptors and induce migration in human endometrial HEC-1B cells. British Journal of Pharmacology 165:2414-2424 DOI 10.1111/j.1476-5381.2011.01497.x.
26. McHugh D, Roskowski D, Xie S, Bradshaw HB. 2014. Delta(9)-THC and N-arachidonoyl glycine regulate BV-2 microglial morphology and cytokine release plasticity: implications for signaling at GPR18. Frontiers in Pharmacology 4:162 DOI 10.3389/fphar.2013.00162.
27. McHugh D, Wager-Miller J, Page J, Bradshaw HB. 2012b. siRNA knockdown of GPR18 receptors in BV-2 microglia attenuates N-arachidonoyl glycine-induced cell migration. Journal of Molecular Signaling 7:10 DOI 10.1186/1750-2187-7-10.
28. Miserey-Lenkei S, Parnot C, Bardin S, Corvol P, Clauser E. 2002. Constitutive internalization of constitutively active agiotensin II AT(1A) receptor mutants is blocked by inverse agonists. Journal of Biological Chemistry 277:5891-5901 DOI 10.1074/jbc. M108398200.
29. Pacher P, Kunos G. 2013. Modulating the endocannabinoid system in human health and disease-successes and failures. FEBS Journal 280:1918-1943 DOI 10.1111/febs.12260.
30. Qin Y, Verdegaal EM, Siderius M, Bebelman JP, Smit MJ, Leurs R, Willemze R, Tensen CP, Osanto S. 2011. Quantitative expression profiling of G-protein-coupled receptors (GPCRs) in metastatic melanoma: the constitutively active orphan GPCR GPR18 as novel drug target. Pigment Cell and Melanoma Research 24:207-218 DOI 10.1111/j.1755-148X.2010.00781.x.
31. Ryberg E, Larsson N, Sjogren S, Hjorth S, Hermansson NO, Leonova J, Elebring T, Nilsson K, Drmota T, Greasley PJ. 2007. The orphan receptor GPR55 is a novel cannabinoid receptor. British Journal of Pharmacology 152:1092-1101.
32. Samuelson LC, Swanberg LJ, Gantz I. 1996. Mapping of the novel G protein-coupled receptor Gpr18 to distal mouse chromosome 14. Mammalian Genome 7:920-921 DOI 10.1007/s003359900272.
33. Shenoy SK, Drake MT, Nelson CD, Houtz DA, Xiao K, Madabushi S, Reiter E, Premont RT, Lichtarge O, Lefkowitz RJ. 2006. beta-arrestin-dependent, G proteinindependent ERK1/2 activation by the beta2 adrenergic receptor. Journal of Biological Chemistry 281:1261-1273 DOI 10.1074/jbc. M506576200.
34. Sheskin T, Hanus L, Slager J, Vogel Z, Mechoulam R. 1997. Structural requirements for binding of anandamide-type compounds to the brain cannabinoid receptor. Journal of Medicinal Chemistry 40:659-667 DOI 10.1021/jm960752x.
35. Takenouchi R, Inoue K, Kambe Y, Miyata A. 2012. N-arachidonoyl glycine induces macrophage apoptosis via GPR18. Biochemical and Biophysical Research Communi-cations 418:366-371 DOI 10.1016/j.bbrc.2012.01.027.
36. Uwada J, Yoshiki H, Masuoka T, Nishio M, Muramatsu I. 2014. Intracellular localization of the M1 muscarinic acetylcholine receptor through clathrin-dependent constitutive internalization is mediated by a C-terminal tryptophan-based motif. Journal of Cell Science 127:3131-3140 DOI 10.1242/jcs.148478.
37. Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris GA. 2003. The G protein-coupled receptor repertoires of human and mouse. Proceedings of the National Academy of Sciences of the United States of America 100:4903-4908 DOI 10.1073/pnas.0230374100.
38. Watts AO, Verkaar F, Van der Lee MM, Timmerman CA, Kuijer M, Van Offenbeek J, Van Lith LH, Smit MJ, Leurs R, Zaman GJ, Vischer HF. 2013. beta-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR. Journal of Biological Chemistry 288:7169-7181 DOI 10.1074/jbc. M112.406108.
39. Yin H, Chu A, Li W, Wang B, Shelton F, Otero F, Nguyen DG, Caldwell JS, Chen YA. 2009. Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay. Journal of Biological Chemistry 284:12328-12338 DOI 10.1074/jbc. M806516200.