Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps)

Nature Protocols

Volumn 11, Issue 2, 2016, Pages 291-301

  Mark, Kevin G ^a   Loveless, Theresa B ^a   Toczyski, David P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DSK2 PROTEIN; F BOX PROTEIN; POLYUBIQUITIN; RAD23 PROTEIN; UBIQUITIN; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG; PROTEIN; PROTEIN BINDING; UBIQUITIN PROTEIN LIGASE;

AMINO TERMINAL SEQUENCE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME SUBSTRATE; IMMUNOPRECIPITATION; IN VIVO STUDY; LIGASE TRAPPING; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PURIFICATION; REVIEW; UBIQUITINATION; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CHROMATOGRAPHY, AFFINITY; IMMUNOPRECIPITATION; POLYUBIQUITIN; PROTEIN BINDING; PROTEINS; UBIQUITIN-PROTEIN LIGASES; UBIQUITINATION;

EID: 84961335574     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.008     Document Type: Review

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