Single cell multiplexed assay for proteolytic activity using droplet microfluidics

Biosensors and Bioelectronics

Volumn 81, Issue , 2016, Pages 408-414

  Ng, Ee Xien ^a   Miller, Miles A ^b   Jing, Tengyang ^a,c   Chen, Chia Hung ^a,d  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c SINGAPORE MIT ALLIANCE FOR RESEARCH AND TECHNOLOGY   (Singapore)

^d Singapore Institute for Neurotechnology (SINAPSE)   (Singapore)

Author keywords

Droplet microfluidics; FRET sensors; Proteolytic activity matrix analysis; Single cell assay

Indexed keywords

CELL CULTURE; CYTOLOGY; DISEASES; DROPS; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYMES; MICROFLUIDICS; STEM CELLS;

COMPUTATIONAL-ANALYTICAL METHODS; DROPLET MICROFLUIDICS; MATRIX ANALYSIS; MULTIPLE ENZYME ACTIVITIES; PROTEOLYTIC ACTIVITIES; RESONANCE ENERGY TRANSFER; SINGLE CELL ASSAYS; SINGLE CELL RESOLUTION;

CELLS;

ADAM PROTEIN; ADAM10 ENDOPEPTIDASE; GELATINASE A; GELATINASE B; PROTEIN ADAM 17; PROTEIN ADAM 8; STROMELYSIN; UNCLASSIFIED DRUG; PEPTIDE HYDROLASE;

ARTICLE; BREAST CANCER CELL LINE; CELL HETEROGENEITY; CONTROLLED STUDY; CYTOLYSIS ASSAY; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME SUBSTRATE; FLUORESCENCE RESONANCE ENERGY TRANSFER; LEUKEMIA CELL LINE; LUNG CANCER CELL LINE; METASTASIS; MICROFLUIDICS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SINGLE CELL ANALYSIS; DEVICES; ENZYME ASSAY; GENETIC PROCEDURES; HUMAN; METABOLISM; MICROFLUIDIC ANALYSIS; TUMOR CELL LINE;

BIOSENSING TECHNIQUES; CELL LINE, TUMOR; ENZYME ASSAYS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; PEPTIDE HYDROLASES; PROTEOLYSIS; SINGLE-CELL ANALYSIS;

EID: 84961178001     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2016.03.002     Document Type: Article

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