An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination

Biotechnology and Bioengineering

Volumn 113, Issue 4, 2016, Pages 724-734

  Del Giudice, Immacolata ^a   Coppolecchia, Rossella ^a   Merone, Luigia ^a   Porzio, Elena ^a   Carusone, Teresa Maria ^a   Mandrich, Luigi ^a   Worek, Franz ^b   Manco, Giuseppe ^a  

^a CNR   (Italy)

^b Bundeswehr Institute for Pharmacology and Toxicology   (Germany)

Author keywords

Detoxification; Lactonase; Pesticide; Phosphotriesterase; Semi rational engineering approach

Indexed keywords

DECONTAMINATION; DETOXIFICATION; HYDROLASES; ORGANIC SOLVENTS; SCAFFOLDS; SOAPS (DETERGENTS);

BIOTECHNOLOGICAL APPLICATIONS; CATALYTIC EFFICIENCIES; LACTONASE; ORGANOPHOSPHATE HYDROLASE; PHOSPHOTRIESTERASES; SEMI-RATIONAL ENGINEERING APPROACH; STRUCTURAL DETERMINANTS; SULFOLOBUS SOLFATARICUS;

PESTICIDES;

ARYLDIALKYLPHOSPHATASE; DETERGENT; GLUCONOLACTONASE; HYDROLASE; ORGANOPHOSPHATE HYDROLASE; ORGANOPHOSPHATE PESTICIDE; PARAOXON; PHOSPHOTRIESTERASE; SOLVENT; UNCLASSIFIED DRUG; MUTANT PROTEIN; PESTICIDE; PHOSPHATASE; PHOSPHORYLPHOSPHATASE;

ARTICLE; CHIMERA; CONTROLLED STUDY; DETOXIFICATION; ENZYME ACTIVITY; ENZYME STABILITY; HYDROLYSIS; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR DOCKING; MOLECULAR EVOLUTION; MUTAGENESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SULFOLOBUS SOLFATARICUS; THERMOSTABILITY; WASTE MANAGEMENT; AMINO ACID SEQUENCE; BIOTRANSFORMATION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR MODEL; PROCEDURES; PROTEIN CONFORMATION;

AMINO ACID SEQUENCE; BIOTRANSFORMATION; DECONTAMINATION; DIRECTED MOLECULAR EVOLUTION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PESTICIDES; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN CONFORMATION; SULFOLOBUS SOLFATARICUS;

EID: 84959540214     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25843     Document Type: Article

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