Immobilization of organophosphohydrolase OpdA from Agrobacterium radiobacter by overproduction at the surface of polyester inclusions inside engineered Escherichia coli

Biotechnology and Bioengineering

Volumn 109, Issue 5, 2012, Pages 1101-1108

  Blatchford, Paul A ^a   Scott, Colin ^b   French, Nigel ^b   Rehm, Bernd H A ^a  

^a MASSEY UNIVERSITY   (New Zealand)

^b Ecosystem Sciences   (Australia)

Author keywords

Bioremediation; Immobilization; Insecticides; Organophosphohydrolase; Pesticides

Indexed keywords

AGROBACTERIUM RADIOBACTER; APPLICATION PERFORMANCE; C-TERMINUS; COEXPRESSED; CONTAMINATED ENVIRONMENT; FUSION PROTEINS; INCREASED TEMPERATURE; LINKER REGION; ORGANOPHOSPHOHYDROLASE; ORGANOPHOSPHORUS PESTICIDE; RALSTONIA EUTROPHA; STEADY-STATE KINETICS;

BACTERIA; BIODEGRADATION; BIOREMEDIATION; BIOTECHNOLOGY; ENCODING (SYMBOLS); ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; HYDROLYSIS; INSECTICIDES; PESTICIDES; POLLUTION; RADIOACTIVE WASTE VITRIFICATION;

ESTERS;

HYBRID PROTEIN; HYDROLASE; ORGANOPHOSPHOHYDROLASE OPDA; PHAA PROTEIN; PHAB PROTEIN; PHAC PROTEIN; POLYESTER; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOREMEDIATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC CODE; NONHUMAN; PROMOTER REGION; PROTEIN ENGINEERING; PROTEIN HYDROLYSIS; RHIZOBIUM RADIOBACTER; STEADY STATE; SURFACE PROPERTY; THERMOSTABILITY;

AGROBACTERIUM TUMEFACIENS; BACTERIOPHAGE T7; CUPRIAVIDUS NECATOR; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; GENE EXPRESSION; METABOLIC ENGINEERING; PESTICIDES; PHOSPHORIC ACID ESTERS; PHOSPHORIC MONOESTER HYDROLASES; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

EID: 84858448799     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24402     Document Type: Article

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