Point Mutation Ile137-Met Near Surface Conferred Psychrophilic Behaviour and Improved Catalytic Efficiency to Bacillus Lipase of 1.4 Subfamily

Applied Biochemistry and Biotechnology

Volumn 178, Issue 4, 2016, Pages 753-765

  Goomber, Shelly ^a   Kumar, Arbind ^a   Singh, Ranvir ^a   Kaur, Jagdeep ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Bacillus subtilis lipase; Homolog modelling; Laboratory evolution; Overlap site directed mutagenesis; Psychrophilic

Indexed keywords

ALKALINITY; BACTERIOLOGY; EFFICIENCY; ENZYMES; ORGANIC POLYMERS; TEMPERATURE;

BACILLUS SUBTILIS; BIOCHEMICAL STUDIES; CATALYTIC EFFICIENCIES; INCREASED FLEXIBILITY; PSYCHROPHILIC; RELATIVE ACTIVITIES; SITE DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY;

ENZYME ACTIVITY;

AMINO ACID; BACTERIAL ENZYME; ILE137 PROTEIN; POLYHISTIDINE TAG; SCATTER FACTOR RECEPTOR; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; ISOLEUCINE; METHIONINE;

ARTICLE; BACILLUS SUBTILIS; CATALYSIS; CATALYTIC EFFICIENCY; CIRCULAR DICHROISM; COLD ACCLIMATIZATION; CRYSTAL STRUCTURE; DIMERIZATION; DNA BASE COMPOSITION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; GENETIC VARIABILITY; HALF LIFE TIME; LOW TEMPERATURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; PSYCHROPHILIC BACTERIUM; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET SPECTROSCOPY; WILD TYPE; BIOCATALYSIS; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR MODEL;

BACILLUS SUBTILIS; BIOCATALYSIS; CLONING, MOLECULAR; ISOLEUCINE; KINETICS; LIPASE; METHIONINE; MODELS, MOLECULAR; POINT MUTATION;

EID: 84959462355     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1907-5     Document Type: Article

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