Improved activity and thermostability of Bacillus pumilus lipase by directed evolution

Journal of Biotechnology

Volumn 164, Issue 1, 2013, Pages 123-129

  Akbulut, Nagihan ^a   Tuzlakoĝlu Öztürk, Merve ^a   Pijning, Tjaard ^b   Işsever Öztürk, Saliha ^a   Gümüşel, Füsun ^a  

^a GEBZE TECHNICAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

3D homology model; Bacillus pumilus lipase; Biocatalyst; Directed evolution; DNA shuffling; Thermostability

Indexed keywords

BACILLUS PUMILUS; DIRECTED EVOLUTION; DNA SHUFFLING; HOMOLOGY MODELS; THERMOSTABILITY;

BACTERIOLOGY; BIOCATALYSTS; DNA; ORGANIC SOLVENTS; SOAPS (DETERGENTS);

LIPASES;

BACILLUS PUMILUS LIPASE; PHENYLALANINE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS PUMILUS; BACTERIAL GENE; DNA PURIFICATION; DNA SEQUENCE; DNA SHUFFLING; ENZYME STABILITY; EVOLUTION; HALF LIFE TIME; HYDROGEN BOND; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMOSTABILITY;

BACILLUS; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ENZYME STABILITY; ESCHERICHIA COLI; HOT TEMPERATURE; LIPASE; MODELS, MOLECULAR; MUTATION; RECOMBINANT PROTEINS; STRUCTURAL HOMOLOGY, PROTEIN;

BACILLUS PUMILUS;

EID: 84875117804     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.12.016     Document Type: Article

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