Mannose metabolism in recombinant CHO cells and its effect on IgG glycosylation

Biotechnology and Bioengineering

Volumn 113, Issue 7, 2016, Pages 1468-1480

  Slade, Peter G ^a   Caspary, R Guy ^a   Nargund, Shilpa ^b   Huang, Chung Jr ^b  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

CHO; Flux analysis; Glycosylation; High mannose; IgG; Mannose; Metabolism

Indexed keywords

CELL GROWTH; CELLS; CYTOLOGY; GROWTH KINETICS; METABOLISM; METABOLITES; PHYSIOLOGY;

ANTIBODY PRODUCTION; FLUX ANALYSIS; HIGH-MANNOSE; INTRACELLULAR CONCENTRATION; MANNOSE; NUTRIENT LIMITATIONS; SPECIFIC PRODUCTIVITY; SYNTHETIC PATHWAYS;

GLYCOSYLATION;

CARBON 13; GLYCAN; HEXOSE; IMMUNOGLOBULIN G; MANNOSE; MANNOSIDASE; GLYCOSYLATED IGG; RECOMBINANT PROTEIN;

AMINO ACID ANALYSIS; AMINO ACID COMPOSITION; AMINO ACID METABOLISM; ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOMASS; CARBOHYDRATE METABOLISM; CARBON BALANCE; CARBON METABOLISM; CARBON SOURCE; CELL GROWTH; CELL METABOLISM; CHO CELL LINE; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; FEMALE; GLYCOLYSIS; GLYCOSYLATION; GOLGI COMPLEX; METABOLOMICS; NONHUMAN; NUTRIENT LIMITATION; NUTRIENT UPTAKE; OSMOLALITY; PENTOSE PHOSPHATE CYCLE; PH; PROTEIN GLYCOSYLATION; ANIMAL; CHEMISTRY; CRICETULUS; HAMSTER; METABOLIC FLUX ANALYSIS; METABOLISM;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GLYCOSYLATION; IMMUNOGLOBULIN G; MANNOSE; METABOLIC FLUX ANALYSIS; RECOMBINANT PROTEINS;

EID: 84958280421     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25924     Document Type: Article

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