Recombinant expression and reconstitution of multiprotein complexes by the USER cloning method in the insect cell-baculovirus expression system

Methods

Volumn 95, Issue , 2016, Pages 13-25

  Zhang, Ziguo ^a   Yang, Jing ^a   Barford, David ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Baculovirus insect cell expression; Multi protein complexes; MultiBac; USER ligation free cloning

Indexed keywords

MULTIPROTEIN COMPLEX; URACIL; RECOMBINANT PROTEIN;

ANIMAL CELL; AUTOGRAPHA CALIFORNICA; BACULOVIRUS EXPRESSION SYSTEM; CODON; CONTROLLED STUDY; GENE DOSAGE; INSECT CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVIEW; SPODOPTERA FRUGIPERDA; TRICHOPLUSIA NI; URACIL SPECIFIC EXCISION REAGENT CLONING METHOD; ANIMAL; BACULOVIRIDAE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROCEDURES; PROTEIN ENGINEERING; SF9 CELL LINE; SPODOPTERA; TRANSGENE;

ANIMALS; BACULOVIRIDAE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SF9 CELLS; SPODOPTERA; TRANSGENES; URACIL;

EID: 84957428884     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.10.003     Document Type: Review

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