Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells

Journal of Structural Biology

Volumn 175, Issue 2, 2011, Pages 198-208

  Vijayachandran, Lakshmi Sumitra ^a   Viola, Cristina ^a   Garzoni, Frederic ^a   Trowitzsch, Simon ^a   Bieniossek, Christoph ^a   Chaillet, Maxime ^a   Schaffitzel, Christiane ^a   Busso, Didier ^b,c,d,e   Romier, Christophe ^b,c,d,e   Poterszman, Arnaud ^b,c,d,e   Richmond, Timothy J ^f   Berger, Imre ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

^b INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^c INSERM   (France)

^d CNRS   (France)

^e UNIVERSITÉ DE STRASBOURG   (France)

^f ETH ZURICH   (Switzerland)

Author keywords

ACEMBL; Automation; Baculovirus; BEVS; High throughput; Insect cells; Mammalian host; MultiBac; PET MCN; Polyprotein; Recombineering; Robotics; Structural biology

Indexed keywords

MULTIPROTEIN COMPLEX; POLYPROTEIN;

ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ESCHERICHIA COLI; EUKARYOTIC CELL; GENE CONSTRUCT; GENE OVEREXPRESSION; INSECT; MAMMAL CELL; NONHUMAN; PIPELINE; PRIORITY JOURNAL; PROKARYOTIC CELL; PROTEIN EXPRESSION; ROBOTICS; STOICHIOMETRY;

ACADEMIES AND INSTITUTES; ANIMALS; AUTOMATION, LABORATORY; BACULOVIRIDAE; CELLS, CULTURED; CLONING, MOLECULAR; ESCHERICHIA COLI; EUROPE; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MULTIPROTEIN COMPLEXES; POLYPROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SPODOPTERA;

EUKARYOTA; HEXAPODA; MAMMALIA; PROKARYOTA;

EID: 79960027830     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2011.03.007     Document Type: Article

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