Molecular cloning and functional characterization of murine toll-like receptor 8

Molecular Medicine Reports

Volumn 13, Issue 2, 2016, Pages 1119-1126

  Li, Tingting ^a   He, Xiaobing ^a   Jia, Huaijie ^a   Chen, Guohua ^a   Zeng, Shuang ^a   Fang, Yongxiang ^a   Jin, Qiwang ^a   Jing, Zhizhong ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Cloning; Expression analysis; Innate immune response; Mouse; Toll like receptor 8

Indexed keywords

ALPHA2 INTERFERON; BETA ACTIN; COMPLEMENTARY DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERFERON REGULATORY FACTOR 3; INTERFERON SENSITIVE RESPONSE ELEMENT; MEMBRANE PROTEIN; RNA; TOLL LIKE RECEPTOR 8; TOLL LIKE RECEPTOR ADAPTOR MOLECULE 1; TRANSCRIPTION FACTOR AP 1; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; MESSENGER RNA; TLR8 PROTEIN, MOUSE; TUMOR NECROSIS FACTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; GENETIC TRANSFECTION; HEART; IMMUNOREACTIVITY; IMMUNOREGULATION; INNATE IMMUNITY; LUNG; MOLECULAR CLONING; MOUSE; NONHUMAN; OPEN READING FRAME; PERIPHERAL BLOOD MONONUCLEAR CELL; PHYLOGENY; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SEQUENCE ALIGNMENT; SPLEEN; TISSUE DISTRIBUTION; ANIMAL; C57BL MOUSE; CHEMISTRY; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE; SEQUENCE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION PROFILING; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; NF-KAPPA B; PHYLOGENY; PROTEIN STRUCTURE, SECONDARY; RNA, MESSENGER; SEQUENCE ANALYSIS, PROTEIN; TOLL-LIKE RECEPTOR 8; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84956881969     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2015.4668     Document Type: Article

References (22)

1. Wu JR and Chen ZJ: Innate immune sensing and signaling of cytosolic nucleic acids. Annu Rev Immunol 32: 461-488, 2014.
2. He XB, Jia HJ, Jing ZZ and Liu DX: Recognition of pathogen-associated nucleic acids by endosomal nucleic acid-sensing toll-like receptors. Acta Biochim Biophys Sin (Shanghai) 45: 241-258, 2013.
3. Unterholzner L: The interferon response to intracellular DNA: Why so many receptors? Immunobiology 218: 1312-1321, 2013.
4. Botos I, Segal DM and Davies DR: The structural biology of toll-like receptors. Structure 19: 447-459, 2011.
5. Lim KH and Staudt LM: Toll-like receptor signaling. Cold Spring Harb Perspect Biol 5: a011247, 2013.
6. Cervantes JL, Weinerman B, Basole C and Salazar JC: TLR8: The forgotten relative revindicated. Cell Mol Immunol 9: 434-438, 2012.
7. Gorden KK, Qiu XX, Binsfeld CC, Vasilakos JP and Alkan SS: Cutting edge: Activation of murine TLR8 by a combination of imidazoquinoline immune response modifiers and polyT oligodeoxynucleotides. J Immunol 177: 6584-6587, 2006.
8. Martinez J, Huang X and Yang Y: Toll-like receptor 8-mediated activation of murine plasmacytoid dendritic cells by vaccinia viral DNA. Proc Natl Acad Sci USA 107: 6442-6447, 2010.
9. Bauer S, Bathke B, Lauterbach H, Pätzold J, Kassub R, Luber CA, Schlatter B, Hamm S, Chaplin P, Suter M and Hochrein H: A major role for TLR8 in the recognition of vaccinia viral DNA by murine pDC? Proc Natl Acad Sci USA 107: E139, 2010.
10. Demaria O, Pagni PP, Traub S, de Gassart A, Branzk N, Murphy AJ, Valenzuela DM, Yancopoulos GD, Flavell RA and Alexopoulou L: TLR8 deficiency leads to autoimmunity in mice. J Clin Invest 120: 3651-3662, 2010.
11. Ma Y, Haynes RL, Sidman RL and Vartanian T: TLR8: An innate immune receptor in brain, neurons and axons. Cell Cycle 6: 2859-2868, 2007.
12. Chuang TH and Ulevitch RJ: Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw 11: 372-378, 2000.
13. Heil F, Hemmi H, Hochrein H, Ampenberger F, Kirschning C, Akira S, Lipford G, Wagner H and Bauer S: Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science 303: 1526-1529, 2004.
14. Jurk M, Heil F, Vollmer J, Schetter C, Krieg AM, Wagner H, Lipford G and Bauer S: Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848. Nat Immunol 3: 499, 2002.
15. Hemmi H, Kaisho T, Takeuchi O, Sato S, Sanjo H, Hoshino K, Horiuchi T, Tomizawa H, Takeda K and Akira S: Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat Immunol 3: 196-200, 2002.
16. Ohto U, Tanji H and Shimizu T: Structure and function of toll-like receptor 8. Microbes Infect 16: 273-282, 2014.
17. Liu J, Xu C, Hsu LC, Luo YP, Xiang R and Chuang TH: A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition. Mol Immunol 47: 1083-1090, 2010.
18. Zhu J, Lai K, Brownile R, Babiuk LA and Mutwiri GK: Porcine TLR8 and TLR7 are both activated by a selective TLR7 ligand, imiquimod. Mol Immunol 45: 3238-3243, 2008.
19. Ziegler-Heitbrock L: The CD14+ CD16+ blood monocytes: Their role in infection and inflammation. J Leukocyte Biol 81: 584-592, 2007.
20. Alexopoulou L, Desnues B and Demaria O: Toll-like receptor 8: The awkward TLR. Med Sci (Paris) 28: 96-102, 2012 (In French).
21. Edwards AD, Diebold SS, Slack EM, Tomizawa H, Hemmi H, Kaisho T, Akira S and Reis e Sousa C: Toll-like receptor expression in murine DC subsets: Lack of TLR7 expression by CD8 alpha+ DC correlates with unresponsiveness to imidazoquinolines. Eur J Immunol 33: 827-833, 2003.
22. Zhu JZ, Brownlie R, Liu Q, Babiuk LA, Potter A and Mutwiri GK: Characterization of bovine Toll-like receptor 8: Ligand specificity, signaling essential sites and dimerization. Mol Immunol 46: 978-990, 2009.