Comprehensive Characterization of AMP-Activated Protein Kinase Catalytic Domain by Top-Down Mass Spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 27, Issue 2, 2016, Pages 220-232

  Yu, Deyang ^a,b   Peng, Ying ^a   Ayaz Guner, Serife ^a   Gregorich, Zachery R ^a,b   Ge, Ying ^a,b  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Activity; AMPK; Electron capture dissociation; Mass spectrometry; Phosphorylation

Indexed keywords

AMINO ACIDS; ASSAYS; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; MASS SPECTROMETRY; METABOLISM; PROTEINS; SPECTROMETRY; SUBSTRATES; THERMODYNAMIC PROPERTIES;

AMP-ACTIVATED PROTEIN KINASE; AMPK; C-TERMINAL TRUNCATION; ELECTRON CAPTURE DISSOCIATION; PHOSPHORYLATION REACTIONS; PHOSPHORYLATION SITES; SEQUENTIAL ORDERING; TOP-DOWN MASS SPECTROMETRIES;

PHOSPHORYLATION;

HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; POLYHISTIDINE TAG; THREONINE; PROTEIN SERINE THREONINE KINASE; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; STK11 PROTEIN, HUMAN;

ARTICLE; AUTOPHOSPHORYLATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ESCHERICHIA COLI; HUMAN; IN VITRO STUDY; MASS SPECTROMETRY; NONHUMAN; PROTEIN DEPHOSPHORYLATION; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR CLONING; PHOSPHORYLATION; PROTEIN SUBUNIT;

AMP-ACTIVATED PROTEIN KINASES; CATALYTIC DOMAIN; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; MASS SPECTROMETRY; PHOSPHORYLATION; PROTEIN SUBUNITS; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; THREONINE;

EID: 84955473066     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-015-1286-8     Document Type: Article

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