Overcoming low yields of plant-made antibodies by a protein engineering approach

Biotechnology Journal

Volumn 11, Issue 1, 2016, Pages 107-116

  Zischewski, Julia ^a   Sack, Markus ^a   Fischer, Rainer ^a,b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

Agroinfiltration; Chimeric VH domain; Plant based expression system; Transient expression; Yield limitations

Indexed keywords

BIOCHEMICAL ENGINEERING; GENE EXPRESSION; GENES; GENETIC ENGINEERING; PLANTS (BOTANY); POLYMERASE CHAIN REACTION; PRODUCTION PLATFORMS; PROTEINS; SURFACE PLASMON RESONANCE;

AGROINFILTRATION; CHIMERIC VH DOMAIN; PLANT-BASED EXPRESSION; TRANSIENT EXPRESSION; YIELD LIMITATIONS;

ANTIBODIES;

MESSENGER RNA; PROTEIN; ANTIBODY; NANOBODY;

ANTIBODY PRODUCTION; ARTICLE; COMPLEMENTARITY DETERMINING REGION; GENE EXPRESSION SYSTEM; GENE SILENCING; GENETIC TRANSCRIPTION; HEAVY CHAIN; HUMAN; NICOTIANA BENTHAMIANA; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN PROCESSING; REAL TIME POLYMERASE CHAIN REACTION; SURFACE PLASMON RESONANCE; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PLANT LEAF; PROCEDURES; TOBACCO; TRANSGENIC PLANT;

ANTIBODIES; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PROTEIN ENGINEERING; SINGLE-DOMAIN ANTIBODIES; SURFACE PLASMON RESONANCE; TOBACCO;

EID: 84955216838     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500255     Document Type: Article

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