Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value

Methods

Volumn 50, Issue 4, 2010, Pages 313-322

  Tuomi, Jari Michael ^a   Voorbraak, Frans ^b   Jones, Douglas L ^a   Ruijter, Jan M ^b  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Cumulative fluorescence; Hydrolysis probes; Measurement bias; PCR efficiency; Quantitative real time PCR

Indexed keywords

ARTICLE; DNA BINDING; FLUORESCENCE; GENE EXPRESSION; HYBRIDIZATION; HYDROLYSIS; MATHEMATICAL MODEL; PRIORITY JOURNAL; REFERENCE VALUE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; CHEMISTRY; EPIDEMIOLOGY; HEART ATRIUM; METHODOLOGY; MOLECULAR PROBE; MOUSE; POLYMERASE CHAIN REACTION;

DIAGNOSTIC AGENT; FLUORESCENT DYE; ORGANIC COMPOUND; RNA; SYBR GREEN I;

ANIMALS; BIAS (EPIDEMIOLOGY); FLUORESCENCE; FLUORESCENT DYES; GENE EXPRESSION; HEART ATRIA; HYDROLYSIS; MICE; MOLECULAR PROBES; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; RNA;

EID: 77649237032     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.02.003     Document Type: Article

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