Generation of knock-in pigs carrying Oct4-tdTomato reporter through CRISPR/Cas9-mediated genome engineering

PLoS ONE

Volumn 11, Issue 1, 2016, Pages

  Lai, Sisi ^a   Wei, Shu ^a   Zhao, Bentian ^a   Ouyang, Zhen ^a   Zhang, Quanjun ^a   Fan, Nana ^a   Liu, Zhaoming ^a   Zhao, Yu ^a   Yan, Quanmei ^a   Zhou, Xiaoqing ^a   Li, Li ^a   Xin, Jige ^c   Zeng, Yangzhi ^c   Lai, Liangxue ^a,b   Zou, Qingjian ^a  

^a GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^b JILIN UNIVERSITY   (China)

^c YUNNAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 9; CRISPR ASSOCIATED PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; RED FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BLASTOCYST; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; FETUS; FIBROBLAST; GENE; GENETIC ENGINEERING; NONHUMAN; NUCLEAR REPROGRAMMING; OCT4 GENE; PIG; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; STOP CODON; ANIMAL; CYTOLOGY; GENE TARGETING; GENE VECTOR; GENETICS; GENOME; GENOTYPE; METABOLISM; NEWBORN; PROMOTER REGION; REPORTER GENE; REPRODUCIBILITY;

ANIMALS; ANIMALS, NEWBORN; BLASTOCYST; CELLULAR REPROGRAMMING; CRISPR-CAS SYSTEMS; FETUS; FIBROBLASTS; GENE KNOCK-IN TECHNIQUES; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GENOME; GENOTYPE; OCTAMER TRANSCRIPTION FACTOR-3; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS; SUS SCROFA;

EID: 84954564294     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0146562     Document Type: Article

References (22)

1. Shi G, Jin Y. Role of Oct4 in maintaining and regaining stem cell pluripotency. Stem Cell Res Ther. 2010; 1(5):39. doi: 10.1186/scrt39 PMID: 21156086; PubMed Central PMCID: PMC3025441.
2. Froschauer A, Khatun MM, Sprott D, Franz A, Rieger C, Pfennig F, et al. oct4-EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka. Mol Reprod Dev. 2013; 80(1):48-58. doi: 10.1002/mrd.22135 PMID: 23139203.
3. Nowak-Imialek M, Kues WA, Petersen B, Lucas-Hahn A, Herrmann D, Haridoss S, et al. Oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies. Stem Cells Dev. 2011; 20(9):1563-75. doi: 10.1089/scd.2010.0399 PMID: 21126163.
4. Huang L, Fan N, Cai J, Yang D, Zhao B, Ouyang Z, et al. Establishment of a porcine Oct-4 promoterdriven EGFP reporter system for monitoring pluripotency of porcine stem cells. Cellular reprogramming. 2011; 13(2):93-8. doi: 10.1089/cell.2010.0069 PMID: 21254851.
5. Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, et al. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science. 2002; 295(5557):1089-92. doi: 10.1126/science.1068228 PMID: 11778012.
6. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNAguided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337(6096):816-21. doi: 10.1126/science.1225829 PMID: 22745249.
7. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013; 339(6121):819-23. doi: 10.1126/science.1231143 PMID: 23287718; PubMed Central PMCID: PMC3795411.
8. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, et al. RNA-guided human genome engineering via Cas9. Science. 2013; 339(6121):823-6. doi: 10.1126/science.1232033 PMID: 23287722; PubMed Central PMCID: PMC3712628.
9. Bikard D, Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, et al. Exploiting CRISPRCas nucleases to produce sequence-specific antimicrobials. Nature biotechnology. 2014; 32(11): 1146-50. doi: 10.1038/nbt.3043 PMID: 25282355.
10. Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, et al. Generation of CRISPR/Cas9-mediated genetargeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci. 2014. doi: 10.1007/s00018-014-1744-7 PMID: 25274063.
11. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, et al. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell. 2014; 156(4):836-43. doi: 10.1016/j.cell.2014.01.027 PMID: 24486104.
12. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell. 2013; 154(6): 1370-9. doi: 10.1016/j.cell.2013.08.022 PMID: 23992847; PubMed Central PMCID: PMC3961003.
13. Krentz NA, Nian C, Lynn FC. TALEN/CRISPR-Mediated eGFP Knock-In Add-On at the OCT4 Locus Does Not Impact Differentiation of Human Embryonic Stem Cells towards Endoderm. PLoS One. 2014; 9(12):e114275. doi: 10.1371/journal.pone.0114275 PMID: 25474420; PubMed Central PMCID: PMC4256397.
14. Zeng R, Zeng YZ. Molecular cloning and characterization of SLA-DR genes in the 133-family of the Banna mini-pig inbred line. Anim Genet. 2005; 36(3):267-9. doi: 10.1111/j.1365-2052.2005.01277.x PMID: 15932417.
15. Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q, et al. Piglets cloned from induced pluripotent stem cells. Cell research. 2013; 23(1):162-6. doi: 10.1038/cr.2012.176 PMID: 23247628; PubMed Central PMCID: PMC3541650.
16. Quan L, Chen Y, Song J, Yan Q, Zhang Q, Lai S, et al. Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System. PloS one. 2014; 9(10):e109728. doi: 10.1371/journal.pone.0109728 PMID: 25360692.
17. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, et al. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature biotechnology. 2004; 22(5):589-94. doi: 10.1038/nbt957 PMID: 15064769.
18. Rogers CS, Stoltz DA, Meyerholz DK, Ostedgaard LS, Rokhlina T, Taft PJ, et al. Disruption of the CFTR gene produces a model of cystic fibrosis in newborn pigs. Science. 2008; 321(5897):1837-41. doi: 10.1126/science.1163600 PMID: 18818360; PubMed Central PMCID: PMC2570747.
19. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, et al. CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014; 159(2):440-55. doi: 10.1016/j.cell.2014.09.014 PMID: 25263330.
20. Ezashi T, Telugu BP, Alexenko AP, Sachdev S, Sinha S, Roberts RM. Derivation of induced pluripotent stem cells from pig somatic cells. Proc Natl Acad Sci U S A. 2009; 106(27):10993-8. doi: 10.1073/pnas.0905284106 PMID: 19541600; PubMed Central PMCID: PMC2698893.
21. Wu Z, Chen J, Ren J, Bao L, Liao J, Cui C, et al. Generation of pig induced pluripotent stem cells with a drug-inducible system. J Mol Cell Biol. 2009; 1(1):46-54. doi: 10.1093/jmcb/mjp003 PMID: 19502222.
22. Sui D, Sun Z, Xu C, Wu Y, Capecchi MR, Wu S, et al. Fine-tuning of iPSC derivation by an inducible reprogramming system at the protein level. Stem cell reports. 2014; 2(5):721-33. doi: 10.1016/j.stemcr. 2014.03.013 PMID: 24936457; PubMed Central PMCID: PMC4050490.