Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-terminal amino acids

Journal of Biotechnology

Volumn 212, Issue , 2015, Pages 92-98

  Wang, Xiong ^a   Han, Shaoqiang ^a   Yang, Zujun ^a   Tang, Lixia ^a  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

C terminal; Halohydrin dehalogenase; Multi site combinatorial mutagenesis; Single site saturation mutagenesis; Truncated mutagenesis

Indexed keywords

AMINO ACIDS; MUTAGENESIS; STABILITY;

AGROBACTERIUM RADIOBACTER AD1; C-TERMINAL REGIONS; HALOHYDRIN DEHALOGENASE; MULTI-SITE; SATURATION MUTAGENESIS; SINGLE POINT; SINGLE SITES; TERMINAL RESIDUES;

ENZYME ACTIVITY;

AMINO ACID; BACTERIAL ENZYME; HALOHYDRIN DEHALOGENASE; UNCLASSIFIED DRUG; HYDROLASE;

AGROBACTERIUM TUMEFACIENS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME STABILITY; GENE DELETION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SUBSTITUTION REACTION; THERMOSTABILITY; AMINO ACID SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HEAT; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; AMINO ACIDS; ENZYME STABILITY; ESCHERICHIA COLI; HOT TEMPERATURE; HYDROLASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SEQUENCE ALIGNMENT;

EID: 84951932280     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.08.013     Document Type: Article

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